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Showing 1 to 20 of 31 for “"Phosphopeptides"”.
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Caged phosphopeptides and phosphoproteins : agents in unraveling complex biological pathways
… system. The syntheses and applications of caged phosphopeptides and phosphoproteins have been developed and detailed within this thesis. Two methods to synthesize 1-(2-nitrophenyl)ethyl caged phosphopeptides were developed. These peptides demonstrated good quantum yields of uncaging as compared …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
Protein phosphorylation is a central regulatory mechanism in signal transduction pathways and cellular migration. Current genetic strategies for the study of phosphorylation, including gene knockout and point mutation, are limited in providing temporal information. As a complement to these …
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Goat and cow casein derived ingredients and their interactions with iron : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Food Technology, Massey University, Palmerston North, New Zealand
… of iron to that of an intact casein micelle. Phosphopeptides were isolated by adding calcium and ethanol to caseinate digests. There was an increase in serine, glutamic acid and isoleucine residues compared to caseinate. There was an increase in phosphorus from 7.8 ± 0.3 mg P/ g solids to 45.4 …
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The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
… myeloid leukaemia is a classic example. As phosphopeptides are known to be presented by both the MHC class-I and class- II pathways, against which specific CD4+ and CD8+ T cell responses may be generated, study of MHC-presented phosphopeptides may reveal unique cancer antigens with direct …
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Development Of Kinase Catalyzed Biotinylation To Study Phosphoproteomics
… where ATP-biotin is utilized by kinases to label phosphopeptides or phosphoproteins with a biotin tag. To explore kinase-catalyzed biotinylation, kinetic measurements were obtained with various kinases and the data indicated that kinase-catalyzed biotinylation occurs with catalytic efficiency …
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The Development of Functionalized Metal Complexes as Selective Phosphopeptide Molecular Recognition Agents
… against a family of high-profile pY-containing phosphopeptides. The reported family of mimetics displayed a wide variance in cytotoxicity against common cancer cell lines, supporting our structure-activity hypothesis. Selectivity observed in our fluorescence intensity assay did not hold in a …
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Development of Methods for the Study of Phosphoproteins
… of this novel column to isolate model phosphopeptides was shown. More general use of this column in MudPIT (strong cation exchange column followed by reverse-phased MS/MS) is probably somewhat limited, due to the hydrophobicity of the AMPS monomer. A better monolith could be obtained if …
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Development of Amine Containing Polymer Modified MALDI Targets for Complex Peptide and Protein Mixture Fractionation Prior to MALDI Mass Spectrometry Analysis
… doped with copper ions to selectively capture phosphopeptides. Negatively charged phosphopeptides were shown to be selectively captured by the copper ions complexes within the polymer brush substrate, and subsequently released upon addition of acid.
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… Mass Spectrometry), and antibodies. To identify phosphopeptides and protein phosphorylation sites proteins were digested with an endoproteinase and subjected to LC/MSn analysis. The determination of protein phosphorylation sites was achieved by making use of the distinct neutral loss of …
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Novel methods in computational analysis and design of protein-protein interactions : applications to phosphoregulated interactions
… application to the study of the interactions of phosphopeptides with phosphopeptide-binding domain interactions. A novel protein-protein interaction type, the action-at-a-distance interaction, is described in the complex of the TEM1 P-lactamase with the 3-lactamase inhibitor protein (BLIP). New …
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Unbiased global proteomic profiling of patient-derived meningiomas of all grades to identify molecular signatures of differentially expressed proteins and phosphoproteins.
… analysing global proteins, phosphoproteins and phosphopeptides. We performed differential expression analyses and functional annotation studies to identify commonly upregulated proteins and phosphoproteins in all grades of meningioma compared to meningeal tissue as well as grade-specific …
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Deoxyribozymes for peptide substrates: exploring the landscape of nucleophiles and electrophiles
… identify deoxyribozymes that can covalently tag phosphopeptides with RNA. These studies are described in Chapter 5. Such deoxyribozymes can be employed as reagents to isolate phosphopeptides from a mixture of peptides. We identified DNA enzymes that catalyze the nucleophilic attack of the …
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Selection of a Non-Phosphorylated Peptide Inhibitor of BRCA1’s (BRCT)2 Domain
… been shown to induce BRCA1 deficient phenotypes. Phosphopeptides have also been studied as potential BRCA1 inhibitors, yet despite some having affinities in the mid-nanomolar range the presence of a phosphate is not without its pharmacologic challenges. We generated an mRNA display library with …
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White spot lesion regression using casein phosphopeptide amorphous calcium phosphate complexes alone or combined with microabrasion.
… (WSLs) can be improved by treatment with casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP). The aim of this study was to test the null hypothesis that the treatment of WSLs with CPP-ACP complexes produces no difference in the outcome of WSL regression when compared to controls …
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Identification of the ERK5 Kinase as a Key Regulator of Embryonic Stem Cell Pluripotency
… a cohort of high confidence ERK5responsive phosphopeptides, I identify a dual phosphosite on the core naïve pluripotency transcription factor KLF2. ERK5 directly phosphorylates KLF2 at tandem Thr-Pro/Ser-Pro motifs, which form consensus binding sites for the Peptidyl-Prolyl Isomerase PIN1. …
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Protein Tyrosine Phosphorylation in Haematopoietic Cancers and the Functional Significance of Phospho- Lyn SH2 Domain
… has reduced ability to interact in vitro with phosphopeptides and phosphoproteins. Sequence analysis of the binding motifs revealed that phosphorylation at Y194 decreased the selectivity of Lyn SH2 for the third residue C-terminal to the pY of the ligand. Together, these data show that tyrosyl …
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Mass Spectrometric Characterization of the MCF7 Cancer Cell Line: Proteome Profile and Cancer Biomarkers
… this device was also capable to analyze phosphopeptides.
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Development of Tyrosine Kinase Peptide Biosensors and Methods for Detection
… transfer (TR-LRET) between the terbium bound phosphopeptides and the orthogonal fluorophore labels.</p>
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Identification of new functions for BRCT domains
… been unable to demonstrate that Esc4p binds to phosphopeptides. However, recent work from the Brown lab has shown that Esc4p fails to be recruited to chromatin after DNA damage in the absence of a histone acetyl-transferase Rttl09. We have demonstrated, using an in vitro binding assay, that the …
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Application of Different Bioanalytical Workflows for Proteomics of Prostate Cancer
… with trypsin; enrichment of the digests for phosphopeptides with immobilized metal ion affinity chromatography (IMAC); analysis of the enriched digests by LC-MS/MS; and identification of the phosphorylated peptides/proteins through searches of the Swiss-Prot protein sequence database. With …
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