Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Phospholipid Vesicles"”.
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Use of Fluorescence to Study the Membrane Binding Domain of Cytochrome B(5) and the Induction of Membrane Domain Formation in Phospholipid Vesicles by Myelin Basic Protein
Embargo set by: Seth Robbins for item 86158 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs
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Multi-Nuclear NMR Studies of Phospholipid Membranes and their Interaction with Membrane Active Substances
… to investigate the interaction of unilamellar phospholipid vesicles with membrane active substances.<br/><br/>The regulation of ion channels by lipids such as the phosphatidic acids was investigated using 1H-NMR and small phospholipid vesicles. Transport across lipid vesicles in the presence of …
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Circular dichroism studies of Escherichia coli pyruvate oxidase and secondary structure and lipid binding properties of the alpha peptide from pyruvate oxidase
… spectra also change with the addition of phospholipid vesicles which bring about an increase in catalytic activity. The circular dichroism spectra for detergent activated and proteolytically activated pyruvate oxidase are virtually identicle indicating there is a further change to the …
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NMR Studies on Model Membranes. Interaction with Metal Ions, Ionophores and General Anaesthetics
… ions was used to investigate the interaction of phospholipid vesicles with membrane active compounds such as ionophores, anaesthetics, surfactants and metal ions.<br/><br/>In chapter 1 an introduction to the NMR technique and current progress in the field of model and biological membranes was …
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High-pressure NMR studies of model membranes
The effects of pressure, up to 5 kbar, on phospholipid vesicles were measured using high pressure NMR techniques. Specifically, two sets of experiments were performed. First, sonicated vesicles of 1-palmitoyl-2-oleyl-3-sn-phosphatidyl-choline at 28$\sp\circ$C were studied using two-dimensional …
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Insulin based inhibitors of human islet amyloid polypeptide (hIAPP) and their effect on aggregation of hIAPP in the treatment of type II diabetes
… against an hIAPP analog in the presence of phospholipid vesicles. While these sequences might inhibit amyloid formation, preliminary results indicate that they actually enhance membrane damage, as measured by the increased leakage of carboxyfluoroscein dye from membrane model vesicles.</p>
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Genetically encoded division machinery for cell free synthetic biology
… water-in-oil emulsion compartments and synthetic phospholipid vesicles. The system was built with DNA and purified transcription and translation machinery housed in a compartment. A particular emphasis was placed on FtsZ, a protein that oligomerizes into a ring at the midcell and splits the cell …
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Application of nanotechnologies for the therapeutic use of natural compounds
… In this context, nanotechnologies, particularly phospholipid vesicles, represent promising strategies to overcome these challenges, enhancing the release, efficacy, and safety of natural products. In addition to extracts derived from traditional medicinal plants, agro-food by-products represent …
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The behaviour of fatty acid vesicles in prebiotically plausible environments
… of model protocells assembled from fatty acid vesicles is lacking in comparison to the abundant literature on phospholipid vesicles. There is evidence that the existence of fatty acids predated life on Earth. Moreover, the highly dynamic nature of fatty acid vesicles is not well documented in …
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Parallel algorithms for direct blood flow simulations
… of red blood cells is similar to that of phospholipid vesicles. Vesicles and red blood cells are both area preserving closed membranes that resist bending. Beyond red blood cells, vesicles can be used to investigate the behavior of cell membranes, intracellular organelles, and viral …
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Activated RhoA Positively Regulates Exchange Activity of PDZ-RhoGEF
… use reconstitution of the signaling pathway with phospholipid vesicles and recombinant proteins, to show that this interaction serves as a mechanism for spatially regulating PRG exchange activity, a feed-forward mechanism. We hypothesize that this feed-forward mechanism is also applicable in vivo …
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Identification of Substrates and Pathways Regulated by WNK1
… the amount of Ca2+ required for Syt2 binding to phospholipid vesicles; mutation of Thr202, a WNK1 phosphorylation site identified from mass spectrometric analysis, partially prevented this change. These findings provide a biochemical scenario that could lead to the retention or insertion of …
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Miniaturized Fluorescence Biosensor for Studying Neuronal Events
… Zn2+ analysis. In the first approach, liposomes (phospholipid vesicles) were used as miniaturized containers for fluorescent sensing reagents. Liposome-based fluorescence sensing technology offers several advantages over commonly used fluorescence sensing techniques including high spatial …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… Both C2 domains interact with negatively charged phospholipids in a Ca2+ dependent manner. The interaction of synaptotagmin 1 with the SNARE complex is also reported. We investigated whether the interaction of synaptotagmin 1 with membranes or with the SNARE complex is critical for membrane …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… Both C2 domains interact with negatively charged phospholipids in a Ca2+ dependent manner. The interaction of synaptotagmin 1 with the SNARE complex is also reported. We investigated whether the interaction of synaptotagmin 1 with membranes or with the SNARE complex is critical for membrane …
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Exploring Novel Techniques for the Single Molecule Toolkit: Vesicle Encapsulation and Immobilization
… DNA inside liposomes (i.e. artificially formed phospholipid vesicles) which were then tethered on the surface. Our data revealed similar behavior for encapsulated and the conventionally attached nucleic acid molecules. Although vesicle encapsulation offers a biologically relevant environment for …
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Role Of Calcium In Membrane Fusion
… 40 years ago, experiments suggested pure phospholipid vesicles may undergo fusion in the presence of Ca<sup>2+</sup>.</p> <p>These experiments have shown that Ca<sup>2+</sup> interacts strongly with apposed bilayers, inducing fusion and</p> <p>forming 1:2 complexes of Ca<sup>2+</sup>/PS. …
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FRET-Based Conformational Sensor for the m1 Muscarinic Cholinergic Receptor
… receptor. Although the sensor reconstituted in phospholipid vesicles retained wild-type agonist binding and signaling function, fluorescence response to agonist was not detected in the vesicles. I demonstrated that solubilization of the sensor denatured a substantial fraction of the sensor, …
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Adsorption Studies of Polysaccharides and Phospholipids Onto Cellulose
… quantitative analysis of polymer supported phospholipid structures as a stable platform for studying membrane-related processes. Smooth cellulose I films were prepared by spincoating cellulose nanocrystal suspensions onto positively charged self-assembled monolayers on gold. The adsorption …
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Studies on intracellular calcium pools in a pancreatic cell line
… acid-sensitive pool. Studies carried out using phospholipid vesicles showed that arachidonic acid and thapsigargin did not alter membrane permeability, indicating that the arachidonic acid- and the thapsigargin-inducedCa²⁺ release was not due to either a Ca²⁺ ionophore or a membrane detergent …
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