Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 31 for “"Phospholipase D"”.
-
Phospholipase D Signaling in T Cells
… of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of …
-
Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the …
-
Phospholipase D as a Factor in Lipid Oxidation of Milk
Made available in DSpace on 2014-12-10T22:38:23Z (GMT). No. of bitstreams: 1 7511720.pdf: 3611139 bytes, checksum: f51ff253d9c3228384d46d1bc09826a6 (MD5) Previous issue date: 1974
-
DEVELOPMENT AND APPLICATION OF CHEMOENZYMATIC TOOLS TO VISUALIZE MAMMALIAN PHOSPHOLIPASE D SIGNALING
… regulation of signal transduction pathways. Phospholipase D (PLD) enzymes are a critical signaling node responsible for production of the pleiotropic lipid second messenger phosphatidic acid via hydrolysis of phosphatidylcholine. It remains a mystery how this one lipid signal can cause such …
-
Attenuation of Corynebacterium pseudotuberculosis by targeted mutagenesis of the phospholipase D gene.
A toxic phospholipase D (PLD), is thought to be an essential virulence determinant of Corynebacterium pseudotuberculosis, an animal pathogen which causes chronic, suppurative infections. In this study, PLD was purified from C. pseudotuberculosis biovar equi isolate 155 and the gene encoding PLD …
-
Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings.
… (NAPE) via the action of phospholipase D (PLD). In an effort to identify a functional NAPE-PLD in cotton seeds and seedlings, we have screened a cotton seedling cDNA (cotyledon mRNA from 48 h dark grown seedlings) library with a 1.2 kb tobacco partial cDNA fragment encoding …
-
Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
The reaction product of phospholipase D (PLD), phosphatidic acid (PA), was found to stimulate phosphatidylinositol-4-phosphate-5-kinase (PIP-5-kinase) activity in vitro. In the present study, we have examined wether PLD affects the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) by …
-
<i>Dictyostelium discoideum</i> Protein Kinase C-orthologue <i>pkcA</i> Regulates the Actin Cytoskeleton through Interaction with Phospholipase D and p21-activated Kinase
<p>Proper regulation of the actin cytoskeleton is crucial to many cellular processes. Many of these processes are regulated by extracellular signaling cues, which direct changes in the actin cytoskeleton, resulting in changes to cellular morphology, and directed motility. The social amoeba, …
-
Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
… acid was observed in frozen cotton fibers. Phospholipase D activity assays revealed that this enzyme readily hydrolyzed radioactive phosphatidylcholine into phosphatidic acid. A profile of expressed sequence tags (ESTs) for genes involved in lipid metabolism in cotton fibers was also …
-
The Effect of The Modification of The Acidic and Zwitterionic Phospholipid Composition on The Enzymatic Activity of D-Beta-Hydroxybutyrate Dehydrogenase
… dehydrogenase (BDH) was examined. Phospholipase D was used to increase the acidic phospholipid composition. This enzyme hydrolyzed primarily zwitterionic phospholipids to phosphatidic acid, an acidic phospholipid. The accumulation of phosphatidic acid increases the electrostatic …
-
BIOCHEMICAL CHARACTERIZATION OF ETHANOL MODIFIED PHOSPHOLIPIDS IN SACCHAROMYCES CEREVISIAE
… UNIQUELY FORMED FROM PHOSPHATIDYLCHOLINE VIA PHOSPHOLIPASE D-CATALYZED TRANSPHOSPHATIDYLATION IN THE PRESENCE OF ETHANOL. IT HAS A DISTINCTLY DIFFERENT CHEMICAL STRUCTURE COMPARED WITH OTHER PHOSPHOLIPIDS AND MAY PERTURB THE PHYSICOCHEMICAL PROPERTIES OF CELL MEMBRANES. PETH HAS BEEN SHOWN TO …
-
Clinical and pathophysiologic studies of increased alkaline phosphatase isoenzymes in the liver and serum of dogs
… by endogenous glycosyl-phosphatidylinositol phospholipase D (GPI-PLD). Release of CALP and LALP into serum by GPI-PLD activity was supported by Western blot analyses using anti-cross reacting determinant antibodies and non-denaturing polyacrylamide gel electrophoresis.
-
D2R Dopamine Receptor Mediates Changes in Dual Specificity Phosphatase Expression in a Small Cell Lung Cancer Cell Line
… suggesting phosphatidic acid (PA) produced from Phospholipase D (PLD) is involved in this induction.</p>
-
Dopamine-induced synaptic plasticity in the amygdala in saline-and cocaine-treated animals undergoing conditioned place preference
… studies have demonstrated a link between phospholipase D (PLD) and metabotropic glutamate receptors (mGluRs), while other studies show an interaction between dopamine receptors (DRs) and PLD. Therefore, we investigated the mechanisms of the dopamine agonist-induced synaptic plasticity that …
-
Novel interactions of the 5-HT₂ₐ and related receptors with intracellular signalling proteins
… GPCRs. The receptor is known to activate phospholipase C (PLC) via the heterotrimeric G proteins Gaq/n, but has been shown to also signal through the phospholipase D (PLD) pathway in an ADP-ribosylation factor (ARF)-dependent manner, that appears to be independent of Gq/n. The M3 …
-
Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells: FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning
… studies suggest that the DUF1669 domain adopts a phospholipase D-like motif, no phospholipase D activity has yet been demonstrated for FAM83 members experimentally. Thus, the role and function of FAM83 proteins remains elusive. This study provides evidence that FAM83 proteins bind to different …
-
Novel anitbody-based assays for disease and food contaminant detection
… based on the detection of antibodies to a phospholipase D (PLD) exotoxin of Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis (CLA), were investigated. A previously cloned recombinant form of PLD (Menzies et al., 1994) was expressed in E. coli, purified and …
-
The role of phospholipase D1 in trafficking and processing of amyloid precursor protein
… is produced by a variety of enzymes, including phospholipase D (PLD). We previously demonstrated that a PLD isoform, PLD2, is required for the synaptotoxic and memory-impairing actions of amyloid beta in an AD mouse model. The role that the other PLD isoform, PLD1, plays in AD is unclear, …
-
Investigating FAM83H, a protein mutated in Amelogenesis Imperfecta
… FAM83 proteins. Although the DU1669 domain has a phospholipase-D-like catalytic motif, no phospholipase-D-like catalytic activity has been found and so FAM83 proteins can be classified as pseudoenzymes. Each FAM83 protein interacts and co-localises with different isoforms of the protein kinase CK1 …
-
Neuropathological significance of PLD1 in Alzheimer's disease and behavioral variant frontotemporal dementia
Aberrantly elevated levels of Phospholipase D (PLD) activity is well documented in neurodysfunctional disorders including Alzheimer’s disease (AD). Our lab established, for the first time, an elevated PLD1 expression in crude synaptosomes from AD post-mortem brains and further demonstrated the …
Page 1 of 2