Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Phosphoglycerate kinase"”.
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Phosphoglycerate kinase 1 as a therapeutic target in motor neuron disease
… the resilience of neurons to disease pathology. Phosphoglycerate kinase 1 (PGK1) is a key enzyme in the first ATP-producing step in the glycolysis pathway. PGK1 may have multiple other roles in cells apart from its role in glycolysis but these are less well understood, and the consequences of …
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Millisecond and Submillisecond Folding Kinetics of Horse Apomyoglobin and Yeast Phosphoglycerate Kinase
Yeast phosphoglycerate kinase is a 415 residue dual domain protein which contains two tryptophans located in the C-terminus. PGK readily cold denatures yielding a state generally devoid of stable secondary structure. Kinetic measurements indicated the molecule collapses to a molten globule type …
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Developing New Chemotherapeutic Agents Against Bone Resorption and Parasitic Diseases Through Computer -Aided Drug Design
… FPPS, soluble vacuolar pyrophosphatase and phosphoglycerate kinase, all important for the proliferation of Trypanosoma brucei, the causative agent of African sleeping sickness. These predictive models aid in optimizing existing inhibitors and ultimately designing novel ones.
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Protein folding in crowded environments and living cells
… We study the folding of the metabolic enzyme phosphoglycerate kinase (PGK) in U2OS (human osteosarcoma) cells and observe variations in folding kinetics within a single cell. FReI experiments carried out on an ensemble of 30 cells reveal large variations in folding time from cell to cell, …
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Glucose metabolism in the outer segments of photoreceptor cells : its involvement in the phototransduction process
… of six glycolytic enzymes including hexokinase, phosphofructokinase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, pyruvate kinase, and lactate dehydrogenase, were found to be present in purified rod outer segment preparations in quantities similar to that …
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Advancing fast relaxation imaging to determine protein stability and folding kinetics on poly(n-isopropyl acrylamide) films
… The protein used in experiments is phosphoglycerate kinase (ePGK1), which undergoes cooperative unfolding. Contrary to previously accepted notions, the melting temperature and kinetic parameters of ePGK1-FRET reveal that the weakly overlapping and mushroom regimes do in fact perturb …
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Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis
… placed under the control of a strong promoter (Phosphoglycerate kinase). Reverse-transcription PCR amplification revealed that the Xvcor transcript accumulated in yeast cells. However, SDS-PAGE analysis could not detect the predicted 29.6 kDa recombinant protein, suggesting that the translation …
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INTERACTION OF CIBACRON BLUE 3G-A AND RELATED DYES WITH NUCLEOTIDE-REQUIRING ENZYMES
… its binding to the selected dehydrogenases and kinases studied is 1-amino-4(4'-aminophenylamino)anthraquinone-2,3'-disulfonic acid. Effective interaction of a structural isomer and smaller moieties of Cibacron Blue 3G-A with these selected kinases and dehydrogenases indicate a largely …
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Protein folding and diffusion: from in vitro to live cells
… and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) in living cells as a function of temperature. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring complete thermal melts and kinetic traces …
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In vitro and in vivo protein folding under stress
… singly-labeled, and a doubly-labeled enzyme PGK (phosphoglycerate kinase). Two mechanisms for non-additivity were proposed. In the “quinary interaction” mechanism, two tags interact transiently with one another, relieving the host protein from unfavorable tag–protein interactions. In the …
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Temperature- and pressure-induced protein dynamics from microseconds to minutes
… how the pressure-temperature phase-diagram of phosphoglycerate kinase is influenced by macromolecular crowding. In Chapter 7, an outreach project is described where simple mechanical and computer models of protein folding were used to teach students at the high school and undergraduate levels …
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Stemness status in differentiated and undifferentiated glioma cells
… They included Uracil DNA glycosylase (UDG), Phosphoglycerate kinase 1 (PGK1), Heterogeneous nuclear riboprotein K (HNRNPK) and moesin that can be used as differentiated markers for glioma cells. Vimentin, Eukaryotic translation initiation factor 4e (EIF4e), Casein kinase II alpha 1 (CSNK2A1) …
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Phase Phenomena of Proteins in Living Matter
… plane. Here, by modeling the protein phosphoglycerate kinase (PGK) on the temperature ($T$), pressure ($P$), and crowding volume-fraction ($\phi$) phase diagram, we demonstrate a critical transition where phases merge, and PGK exhibits large structural fluctuations. Above the critical …
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Protein folding in living cells and under pressure
… folding and the thermodynamic stability of yeast phosphoglycerate kinase (PGK-FRET) are cell cycle-dependent, a process strictly regulated in time, suggesting that the interplay between the intracellular environment and proteins may impact their function. In chapter 6, a new probe to study protein …
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Multi-domain Protein Unfolding Pathway Studies by Single Molecule Techniques
… of two homologous multi-domain proteins – yeast phosphoglycerate kinase (PGK) and its E.coli homolog. I found that yeast PGK has much higher mechanical stability than E.coli PGK although previous literature reported that they had similar thermodynamics stability determined by bulk measurements. …
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Synthesis and kinetic processes in designed polymers
… investigated a crowding sensor protein and phosphoglycerate kinase to quantify the confinement effect of the cross-linked hydrogel and reveal the effect of noncovalent interactions of the protein with the polymer surface. Additionally, we demonstrate that a biomedically-relevant zwitterionic …