Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Phosphoglycerate"”.
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Phosphoglycerate kinase 1 as a therapeutic target in motor neuron disease
… the resilience of neurons to disease pathology. Phosphoglycerate kinase 1 (PGK1) is a key enzyme in the first ATP-producing step in the glycolysis pathway. PGK1 may have multiple other roles in cells apart from its role in glycolysis but these are less well understood, and the consequences of …
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The role of phosphoglycerate dehydrogenase in cell proliferation and tumor progression
… metabolic program to support proliferation. Phosphoglycerate dehydrogenase (PHGDH) catalyzes the first step in the serine biosynthesis pathway, and the gene encoding this enzyme is amplified in human cancer. PHGDH-amplified cells depend on PHGDH expression to proliferate, and upon PHGDH …
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Millisecond and Submillisecond Folding Kinetics of Horse Apomyoglobin and Yeast Phosphoglycerate Kinase
Yeast phosphoglycerate kinase is a 415 residue dual domain protein which contains two tryptophans located in the C-terminus. PGK readily cold denatures yielding a state generally devoid of stable secondary structure. Kinetic measurements indicated the molecule collapses to a molten globule type …
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The Effects of 3-Phosphoglycerate and Other Metabolites on the Activation of AMP-Activated Protein Kinase by LKB1/STRAD/MO25
… (F6P), fructose 1,6-bisphosphate (F1,6-P2), 3-phosphoglycerate (3PG), glucose-1-phosphate (G1P), glucose-1,6-bisphosphate (G1,6-P2), adenosine diphosphate (ADP), carnitine (Carn), acetyl-carnitine (Acarn), inosine monophosphate (IMP), inosine, and ammonia for allosteric regulation. We found …
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Yeast cell wall proteomics: a tale of two proteins.
… result of stress. Two such proteins were found, phosphoglycerate mutase and Hsp 12. Studies on these proteins are reported in chapters 2 (phosphoglycerate mutase) and chapter 3 (Hsp 12).
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Developing New Chemotherapeutic Agents Against Bone Resorption and Parasitic Diseases Through Computer -Aided Drug Design
… FPPS, soluble vacuolar pyrophosphatase and phosphoglycerate kinase, all important for the proliferation of Trypanosoma brucei, the causative agent of African sleeping sickness. These predictive models aid in optimizing existing inhibitors and ultimately designing novel ones.
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Defining sources of nutrient limitation for tumors
… of the serine synthesis pathway enzyme phosphoglycerate dehydrogenase (PHGDH).
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Regulation of transcription of SerA in Escherichia coli K-12
The serA gene in Escherichia coli encodes 3-phosphoglycerate dehydrogenase, which is the first enzyme in the serine biosynthesis pathway. Previous studies have determined two different transcription initiation sites for the serA gene, corresponding to the promoters of serA P1 and P2, and suggested …
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Protein folding in crowded environments and living cells
… We study the folding of the metabolic enzyme phosphoglycerate kinase (PGK) in U2OS (human osteosarcoma) cells and observe variations in folding kinetics within a single cell. FReI experiments carried out on an ensemble of 30 cells reveal large variations in folding time from cell to cell, …
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Glucose metabolism in the outer segments of photoreceptor cells : its involvement in the phototransduction process
… glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, pyruvate kinase, and lactate dehydrogenase, were found to be present in purified rod outer segment preparations in quantities similar to that found in human red blood cells. Immunofluorescence microscopy of bovine and …
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Advancing fast relaxation imaging to determine protein stability and folding kinetics on poly(n-isopropyl acrylamide) films
… The protein used in experiments is phosphoglycerate kinase (ePGK1), which undergoes cooperative unfolding. Contrary to previously accepted notions, the melting temperature and kinetic parameters of ePGK1-FRET reveal that the weakly overlapping and mushroom regimes do in fact perturb …
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Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis
… placed under the control of a strong promoter (Phosphoglycerate kinase). Reverse-transcription PCR amplification revealed that the Xvcor transcript accumulated in yeast cells. However, SDS-PAGE analysis could not detect the predicted 29.6 kDa recombinant protein, suggesting that the translation …
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Application of hydrogen deuterium exchange mass spectrometry in protein-ligand and protein-protein interactions
… The GT-A glycosyltransferase glucosyl-3-phosphoglycerate synthase from Mycobacterium tuberculosis (MtGpgS) catalyzes the first step of biosynthesis of 6-O-methylglucose lipopolysaccharides (MGLPs), which are essential to growth and existence of mycobacterium. The HDX-MS data revealed that …
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Investigation of an energetic coupling between ligand binding and protein folding
… <em>Escherichia coli</em> cofactor-dependant phosphoglycerate mutase (dPGM) was identified as an ATP binding protein in a proteome-wide screen, but dPGM does not use ATP for catalysis. This dissertation elucidates the effect of ATP and other non-substrate metabolites on dPGM. Initial …
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INTERACTION OF CIBACRON BLUE 3G-A AND RELATED DYES WITH NUCLEOTIDE-REQUIRING ENZYMES
… patterns exhibited by Cibacron Blue 3G-A with phosphoglycerate kinase allowed a clear demonstration of the random mechanism of that enzyme. Two new columns were developed for use in the purification of mainly nucleotide requiring enzymes using dyes covalently bound to an agarose matrix. These …
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Protein folding and diffusion: from in vitro to live cells
… and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) in living cells as a function of temperature. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring complete thermal melts and kinetic traces …
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Functional discovery and promiscuity in the RuBisCO superfamily
… 1,5-bisphosphate to yield two molecules of 3-phosphoglycerate. Currently, there are four forms of RuBisCO, three of which catalyze the canonical carboxylation reaction. Form IV RuBisCOs (also known as RuBisCO-like proteins (RLPs)) do not catalyze the carboxylation reaction and are of interest …
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In vitro and in vivo protein folding under stress
… singly-labeled, and a doubly-labeled enzyme PGK (phosphoglycerate kinase). Two mechanisms for non-additivity were proposed. In the “quinary interaction” mechanism, two tags interact transiently with one another, relieving the host protein from unfavorable tag–protein interactions. In the …
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