Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Phosphatase inhibitor"”.
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Protein Phosphatase Inhibitor-1 and Cdk5: Of Molecules and Memory
Protein phosphatase inhibitor-1 and cyclin-dependent kinase 5 (Cdk5) have been independently implicated in synaptic plasticity, learning, and memory. We began our studies with the identification, confirmation, and characterization of a novel Cdk5-dependent phosphorylation site (Ser6) on …
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Development Of Kinase Catalyzed Biotinylation To Study Phosphoproteomics
… Next, the susceptibility of the biotin tag to phosphatases was characterized and found that the phosphorylbiotin group was relatively insensitive to protein phosphatases. Importantly, robust kinase-catalyzed biotinylation occurs without the need for phosphatase inhibitor treatment. The results …
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Small Therapeutic Peptides: In vitro pharmacokinetics of alpha-carboxyl terminus 11 peptide in rat plasma
… in rat plasma in the presence of protease and phosphatase inhibitor cocktails to provide a method to delay its degradation and to understand the degradation pattern of the peptide in vitro. The effect of time, temperature, presence of inhibitors and sex are investigated. Results have shown that …
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TNFalpha expression in monocytes/macrophages
… inhibition. Okadaic acid, a protein phosphatase inhibitor, seems to overcome the posttranscriptional inhibition exerted by dexamethasone when TNF expression is induced. The posttranscriptional regulation of TNF was further studied in human monocytic THP-1 cells. THP-1 cells express …
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Sublethal Hepatic Pathology and Evidence of Intranuclear Effects of the Cyanobacterial Toxin Microcystin -Lr
Microcystin-LR (MCLR) is a hepatotoxin and phosphatase inhibitor produced by Microcystis aeruginosa. The pathology of sublethal exposure is not well characterized. MCLR immunohistochemistry (MCLR-IHC) studies indicate that the nucleus may be a target of MCLR. Balb/c mice were given one sublethal …
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The Differential Contribution of Striatonigral and Striatopallidal Neurons in Mediating Responses to Therapeutic Agents and Drugs of Abuse: A Dual Role for DARPP-32
… consequences. DARPP-32 is a dual function kinase/phosphatase inhibitor which has been shown to be a key mediator of signaling in both striatonigral and triatopallidal neurons. A variety of therapeutic agents and drugs of abuse can affect the phosphorylation of DARPP-32. Psychostimulants such as …
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The analysis of B cell antigen receptor (BCR) mutants deficient in antigen-independent signaling reveals novel BCR-associated proteins
… be analyzed by <br>stimulating B cells with the phosphatase inhibitor pervanadate (PV). Analysis of substrate <br>tyrosine phosphorylation after PV-treatment of different B cell lines expressing BCR mutants <br>revealed drastic differences in phosphorylation of two signaling proteins namely the …
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Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions
… adhesions. Okadaic acid, a serine, threonine phosphatase inhibitor, enhances the contracted phenotype, even at low concentrations (0.5mM) of ATP. The localization of focal adhesion components is different in okadaic acid treated cells. In highly contracted cells, integrin is present in tracks …
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Angiotensin-(1-7): A Peptide Hormone Inhibitor for the Treatment of Breast Cancer Targeting the Tumor Microenvironment
… the mitogen-activated protein (MAP) kinase phosphatase DUSP1 in tumor-associated fibroblasts, by 252 ± 29% compared with untreated cells, with an associated 50% decrease in activities of the MAP kinases ERK1 and ERK2 [1.13 ± 0.23 relative density units (RDU) in untreated cells compared 0.60 …
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Regulation of Protein Kinases (Syk and PKC zeta) in platelets
… not significantly affected. Furthermore, the inhibitory action of curcumin on the catalytic activity of Syk was independent of any of its effects on the thromboxane generation because all our studies were performed using aspirin-treated platelets. PKC zeta is an atypical member of the PKC …
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Transcriptional regulation of the human alpha 2(I) procollagen gene
… of nuclear extracts with calf intestinal phosphatase resulted in a loss of complex I formation on the human and CBF binding to the mouse promoters. The Ser/Thr phosphatase, PP2A, specifically inhibited complexes II and ill formation. Nuclear extracts from CT-1 and U937 cell lines treated …
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Stability Study of Phoslactomycin B and Analysis of Degradation Products
Phoslactomycin B (PLM-B), a potent and selective inhibitor of serine threonine phosphatase is of interest for its antitumor, antifungal and antiviral activity. The objective of this study was to evaluate the stability of phoslactomycin B at various pH and temperature conditions. Phoslactomycin B …
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The eIF2 phosphatase: characterization and modulation
… ISR is PPP1R15A, a regulatory subunit of Protein Phosphatase 1 (PP1). The PP1/PPP1R15A holophosphatase dephosphorylates eIF2 and terminates the ISR once the stresses are resolved. Hence, eIF2 kinases and phosphatases work together to control levels of phosphorylated eIF2. Maintaining the right …