Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Phosducin"”.
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Effect of Melatonin and Dopamine in Site Specific Phosphorylation of Phosducin in Intact Retina
Phosducin (Pdc) is a 28 kDa binding partner for the G protein beta gamma subunit dimer (G-beta-gamma) found abundantly in the photoreceptor cells of the retina and pineal gland. In the retina, light-dependent changes in cAMP and Ca2+ control the phosphorylation of Pdc at serine 73 and 54, …
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The Role of Phosducin-like Protein and the Cytosolic Chaperonin CCT in G beta gamma dimer Assembly
<p>Phosducin-like protein (PhLP), a G protein beta gamma subunit dimer binder and G protein signaling regulator, was suggested to regulate the activity of cytosolic chaperonin CCT by their high affinity interaction. In the present study, the three-dimensional structure of PhLP:CCT complex has been …
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The Role of Phosducin-like Protein as a Co-chaperone with the Cytosolic Chaperonin Complex in Assembly of the G Protein βγ Subunit Dimer
<p>Phosducin-like protein (PhLP) has been shown to interact with the cytosolic chaperonin containing TCP-1 (CCT), and the βγ subunit dimer of heterotrimeric G proteins (Gβγ). Here we provide details obtained from cryo-electron microscopic and biochemical studies on the structure of the complex …
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The Mechanism of Assembly of the G-Protein Beta Gamma Subunit Dimer by CK2 Phosphorylated Phosducin-Like Protein and the Chaperonin Containing TCP-1
Phosducin-like protein (PhLP) binds G-protein beta gamma subunits and is thought to assist in assembly of the G-protein beta gamma dimer. Phosphorylation of PhLP at serine residues 18-20 by the casein kinase 2 (CK2) appears to play an essential role in this process. PhLP has also been shown to …
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Towards Early State Disease Detection in Microdevices: Fabrication and Testing of Micro Total Analysis Systems for Bioanalytical Applications
… TPE microchips had good reproducibility. Phosducin-like protein and phosphorylated phosducin-like protein were also separated to measure the phosphorylation efficiency. My results show that PEG-grafted TPE microchips have broad potential application in biomolecular analysis. Cancer marker …
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Role of Chaperonin CCT in G-protein Biosynthesis
… (G protein) signaling. The CCT co-factor, phosducin-like protein (PhLP) is required for Gbeta biosynthesis, and therefore its mechanism is critical for our understanding of this process. In the first chapter of my thesis, I take a structure-based approach to investigate the interaction …
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Heterotrimeric G protein beta:gamma bound to a biologically active peptide : structural definition of a preferred protein interaction surface
… include Gα, phosphotidylinositol 3 kinase, phosducin, phospholipase C β, adenylyl cyclase, G protein receptor kinase, G protein regulated inwardly rectifying channels, and N and P/Q type calcium channels. The 2.7Å crystal structure of Gβ1γ2 bound to a peptide (SIGKAFKILGYPDYD) obtained from …
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Development of Methods for the Study of Phosphoproteins
… tryptic peptide from CK2-phosphorylated phosducin-like protein (PhLP) was well characterized using enrichment with a MonoTip® TiO Pipette Tip. Analysis of syntaxin 1a phosphorylation by AMPK (AMP-activated protein kinase) was done by peptide level mapping for potential phosphopeptides …