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Showing 1 to 20 of 36 for “"Phorbol 12-myristate 13-acetate"”.

  1. Molecular Mechanisms of Promyelocyte Maturation: Implications for Immune Dysfunction in Type II Diabetes

    Deszo, Eric LouisUniversity of Illinois at Urbana-Champaign2015

    … of CD86 and the production of cytokines in phorbol-12-myristate-13-acetate (PMA)-stimulated promyelocytes is regulated by calcium calmodulin kinase kinase alpha. These data together indicate that promyelocyte differentiation is a process that is dependent on the activity of PKCdelta. …

    uiuc Repository record

  2. Measuring the Changes in Tumor Necrosis Factor-Alpha (TNF-α) from Secretory Populations of U937 Monocytic Cells during Differentiation.

    Tran, An Xuong2002

    … differential effect of retinoic acid (RA) and phorbol 12-myristate 13-acetate (PMA) on the induction of TNF-α secretion from U937 monocytic cell populations by using the reverse hemolytic plaque assay (RHPA). The RHPA will allow us to investigate both changes in TNF-α secreting populations as …

    etsu Repository record

  3. Methylation Change of Histone H3 and CpG Sites at the TIM-3 Gene Promoter in Response to Stimulation

    전, 가정2011

    … TIM-3 유전자 DNA CpG 메틸화 정도를 측정하였을 때 TIM-3 유전자 -1240 ~ + 31 (-242, -259 제외)의 CpG 메틸화가 상대적으로 낮게 관찰되었다. 또한 TIM-3 promoter -242와 -259의 CpG 메틸화 정도가 TIM-3의 발현에 차이가 있는 세포마다 다르게 관찰되었다. 그러나 phorbol 12-myristate 13-acetate (PMA)와 ionomycin (A23187) 자극에 의한 T 세포 활성화에 따른 DNA 메틸화 변화는 일어나지 않았다. 다음으로 TIM-3 …

    ajou Repository record

  4. The Involvement of Interleukin-1 Receptor-Associated Kinase-1 (IRAK-1) as a Critical Modulator of Macrophage Migration

    Gan, LuVirginia Tech2010

    … migration induced by a potent PKC activator, phorbol 12-myristate 13-acetate (PMA), or lipopolysaccharide (LPS) was significantly decreased in IRAK-1-/- murine macrophages compared with wild type cells. Mechanistically, we first demonstrated that IRAK-1 works downstream of PKCε and directly …

    vt Repository record

  5. MECHANISM OF TRANSCRIPTIONAL REPRESSION OF MMP-9 BY INTERFERON-β

    Mittelstadt, Megan Laurel

    … (IFN-beta) downregulates both the basal and phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 expression at the transcriptional level, the molecular mechanism of this repression is poorly understood. We describe here a novel mechanism for repression of MMP-9 transcription by IFN-beta in …

    south-carolina Repository record

  6. Analysis of human cytomegalovirus in the healthy human carrier

    Taylor-Wiedeman, JeanThe Open University1992

    … MDM treated with hydrocortisone (HC) and phorbol 12-myristate 13-acetate, expressed only lEI, but not IE2, glycoprotein B (gB) or phosphoprotein 28 (pp28) transcripts. Whereas, MDM treated with granulocyte-macrophage colony stimulating factor and HC expressed lEI, IE2 and gB, but not pp28 …

    the-open-u Repository record

  7. Functional Investigation of Dual αvβ3 and αllbβ3 Integrin Inhibition in Haematological and Solid Tumour Models

    Elsharif, Amal A.M.University of Bradford2018

    … detachment and migration were determined. Phorbol 12-myristate 13-acetate (PMA)-treated K562 cells showed increased adhesion on fibrinogen compared to untreated cells. Adhesion of cancer cells (K562 ± PMA and MCF-7) to fibrinogen was inhibited and detachment was induced by the known β3 …

    bradford Repository record

  8. An unbiased proteomic approach to identifying cell surface markers in neutrophils

    Subburayalu, JulienUniversity of Cambridge2022

    … THP-1, which had been previously primed with phorbol-12-myristate-13-acetate (PMA) to acquire the functional properties of human macrophages. Efferocytosis was assessed by confocal microscopy and flow cytometry. Results: The late-stage apoptotic neutrophil plasma membrane profile identified …

    cambridge Repository record

  9. Identification of a Minimal Cis-element and Cognate Trans-factors Required for the Regulation of Rac2 Gene Expression during K562 Cell Differentiation

    Muthukrishnan, Rajarajeswari2009

    … leukemia cell line K562 as the model system. Phorbol 12-myristate 13-acetate (PMA) stimulation of K562 cells resulted in increased Rac2 mRNA expression as analyzed by real time-polymerase chain reaction (RT-PCR). Luciferase reporter gene assays revealed that increased transcriptional activity …

    iupui Repository record

  10. Single-cell Functional Profiling of Lymphocytes for Cancer Immunotherapy

    An, XingyueUniversity of Houston2019

    … gamma (IFN-γ) secretor upon activation by phorbol 12-myristate 13-acetate (PMA) and ionomycin (< 3 h), and no evidence of cooperation between NK cells to synergistic activation or faster IFN-γ secretion. These results establish our technology as an investigational tool for cellular …

    houston Repository record

  11. Angiotensin II-mediated Regulation of the Human Angiotensin II Type 1 Receptor Gene

    Victor, Xylophone Vijai Aasee2005

    … (H295R cells). Protein kinase C (PKC) activator, phorbol-12-myristate-13-acetate (PMA), showed that the downregulation of hAT1R mRNA is mediated by the PKC pathway. Experiments performed in the presence of cycloheximide and/or Ang II demonstrated that protein translation is essential for hAT1R …

    byu Repository record

  12. New stimulators and a new mechanism of regulated secretion in pituitary gonadotropes

    Billiard, Julia, 1967-1997

    … other hand, a protein kinase C (PKC) activator--phorbol-12-myristate-13-acetate (PMA)--caused LH release from pituitary gonadotropes without evoking changes in (Ca$\sp{2+}\rbrack\sb{i}$. After 1 hour incubation in RHPA, PMA elicited an increase in LH secretion comparable to that caused by GnRH. A …

    washington Repository record

  13. RAF-1 kinase inhibitor protein-mediated cholecystokinin-2 receptor desensitization and extracellular signal-regulated kinase activation

    Jeseong ParkThe University of Texas Medical Branch2009

    … associated onto GRK2 when PKC was activated by phorbol 12-myristate 13-acetate (PMA) treatment, but either G17 stimulation or Gö6976 did not affect on the association. It suggests that PMA-sensitive PKC isozymes are responsible for RKIP phosphorylation; however, CCK2R-mediated PKC isozymes are …

    utmb Repository record

  14. An assessment of the effects of novel anti-inflammatory compounds in cell based studies

    Gafri, Fadia MohamedUniversity of Strathclyde2012

    … plasmids, tumour necrosis factor-α (TNF-α) and phorbol-12-myristate- 13 acetate (PMA) well characterized stimuli for canonical NF-κB pathway induced NF-κB and AP-1 transcriptional activity respectively. However, all tested compounds inhibited NF-κB transcriptional activity; in particular …

    strathclyde Repository record

  15. Molecular Characterization of Dopamine Transporter Phosphorylation and Regulation

    Gorentla, Balachandra Kumar

    … of Protein kinase C (PKC) activators such as (phorbol 12-myristate 13-acetate) PMA. Serine(s) and threonine(s) are known to be involved in DAT phosphorylation.</p> <p>Pretreatment with psychostimulant substrates such as AMPH and METH have been shown to regulate DAT phosphorylation and DA …

    nodak Repository record

  16. Investigation of Immune Response to Sarcocystis neurona Infection in Horses with Equine Protozoal Myeloencephalitis

    Yang, JibingVirginia Tech2005

    … incorporation, to a non-antigen specific mitogen phorbol 12-myristate 13-acetate (PMA) / ionomycin (I) than did clinically normal horses (p=0.04). The implications of these findings will be discussed. In experiment 2, 13 horses were randomly divided into two groups. Baseline neurologic …

    vt Repository record

  17. Identification of a novel method for differentiating human monocytic cell line into macrophages

    Jeddi, MarjanLondon Metropolitan University2020

    … An In vitro protocol was established using phorbol 12-myristate-13-acetate (PMA) and was compared to a conditioned medium model using THP-1 and HUH7 hepatoma cells. Method: THP-1 cells were incubated with 100nM of PMA for 72 hours to differentiate into M0 macrophage-like cells. The novel …

    london-metro Repository record

  18. Characterization of interleukin-4 induced gene-1 (IL-4I1) as a host immunoregulator to cutaneous leishmaniasis in cell based models

    Fouad, AyaDepartment of Molecular and Cell Biology2022

    … stimulation of cytokines such as IL-4 and IL-13 and release of urea that supports parasite growth. Candidate genes responsible for promoting M1 or M2 macrophages are being investigated as the macrophage is the sight of infection for leishmaniasis. One such candidate is IL-4i1, which has been …

    cape-town Repository record

  19. A neutral protease of the neutrophil surface : role in the proteolysis of C-reactive protein and fibrinogen

    Kelly, Sharon LesleyUCT/MRC Liver Research Centre1995

    … the concomitant degradation of this ligand by a phorbol 12-myristate 13 acetateactivatable membrane-associated neutral protease has been shown in previous studies. Degradation of C-reactive protein by the neutrophil protease was shown to result in peptides with an ability to modulate various …

    cape-town Repository record

  20. A study of the metabolic immune responses produced by some natural products

    Alqarni, Abdulmalik M. H.University of Strathclyde2020

    … study, THP-1 cells were differentiated using phorbol 12-myristate 13-acetate (PMA) for 48 hours and then rested for 24 hours by replacing the PMA-containing culture medium with normal medium. The cells were then treated with LPS alone, a natural product alone or a combination of the natural …

    strathclyde Repository record

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