Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Phage lambda"”.
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Cellular decision making: from phage lambda to stem cells
… making and cell-state maintenance, bacteriophage lambda and mouse embryonic stem cells. Bacteriophage lambda (phage lambda) is a bacterial virus that, upon infection of its host bacterium, Escherichia coli, decides between two alternative pathways: The phage can replicate and kill the host …
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Determination of cell fate selection during phage lambda Infection
Bacteriophage lambda infection of Escherichia coli can result in distinct cell fate outcomes: for example, some cells lyse while others survive as lysogens. A quantitative molecular model of lambda infection supports the hypothesis that spontaneous differences in the timing of individual molecular …
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Quantifying transcriptional regulation in Escherichia coli and phage lambda at single-cell, single-molecule resolution
… by the bacterium Escherichia coli and its virus, phage lambda.
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Bacteroides Conjugative Transposons: Ecology and Mechanisms
… though the integrase of CTnDOT belongs to the lambda integrase family of recombinases, the mechanism of integration of CTnDOT is different from that of phage lambda, the model for site-specific recombination systems. Although IntDOT is sufficient for integration in vivo, recent studies have …
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Molecular analysis of the salmon pathogen Piscirickettsia salmonis
… a recombinant library of P. salmonis DNA in a phage lambda vector. P. salmonis LF-89 was grown in CHSE-214 and RTG-2 tissue culture cells and morphological changes monitored by light microscopy and staining. Cytoplasmic vacuoles were visible in infected cells by 5 days post-infection and these …
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The Noncontractile Phage Tail-like Bacterial Killing Nanomachines – Characterizing the Specificity Determinants of the F-Pyocins of Pseudomonas aeruginosa
… this work, I characterized the non-contractile phage tail-like entities called F-pyocins. F-pyocins are high molecular weight bacteriocins resembling non-contractile bacteriophage tails such as E coli phage lambda of the Siphoviridae family. They are produced by many Pseudomonas aeruginosa …
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Studies of Lipoic Acid Metabolism in Escherichia Coli
… was subsequently introduced by transduction with phage lambda particles containing a lipA cosmid. Analysis of the isolated E2 domains by mass spectroscopy and lipoic acid bioassay revealed that the d15-labeled octanoyl-E2 domain had been converted to d13-labeled lipoyl-E2 domain species. No lipoyl …
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Characterization of IntDOT, the CTnDOT Integrase
… tyrosine recombinases such as that of phage lambda. To assess the importance of the conserved residues, I changed residues in IntDOT that align with conserved residues in the tyrosine recombinases. Some substitutions resulted in a complete loss or significant decrease of integration …
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… share similar membrane topologies to that of the phage lambda holin. However, TpeE in C. perfringens shares a similar membrane topology to TatA, a key protein in the Twin Arginine Transport (Tat) secretion system. This thesis tests two models, a pore-forming model and a membrane destabilization …
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Structural insights into RNA binding by NusA and interaction studies of Nun with E. coli Nus factors.
In phage lambda, antitermination is initiated by the lambda-encoded N protein which recruits a number of host proteins called Nus factors. Several of these host proteins which are essential for effective transcription termination and antitermination have been identified, these includes NusA, NusB, …
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The Deoxythymidine Kinase-Deoxythymidylate Kinase Enzymes in SALMONELLA POTSDAM and ESCHERICHIA COLI K12 Infected with Bacteriophage P3
… last thirty years. The virulent T-even bacteriophages have been studied the most and have been shown to be responsible for the induction of a number of new, virus-specific enzymes that are necessary for successful phage growth. In addition, the activity of other host enzymes present prior to …