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Showing 1 to 11 of 11 for “"PfHsp70-1"”.
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Investigation of the role of the GGMP motif of Plasmodium falciparum Hsp70-1 on the chaperone function of the protein and its interaction with a co-chaperone, PfHop
… agent, Plasmodium falciparum expresses an Hsp70 (PfHsp70-1) which plays a significant role in parasite survival. PfHsp70-1 is distinct in that it possesses glycine-glycine-methionine-proline (GGMP) tetrapeptide repeats in its C-terminal domain. To date, the GGMP motif of PfHsp70-1 has not been …
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The development of a molecular chaperone-based system to improve the heterologous production of Plasmodium falciparum AdoMetDC protein in E. coli.
… protein 70 (Hsp70) from Plasmodium falciparum (PfHsp70-1), has been previously shown to exhibit chaperone function when heterologously expressed in E. coli cells whose endogenous Hsp70 function was compromised. PfHsp70-1 presumably protected the E. coli cells against thermal stress by preventing …
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Characterization of Plasmodium falciparum PFF1010c and screening of pyrimidine-quinoline hybrids as inhibitors of HsP70-HsP40 functional partnerships of the Malaria parasite
… drug targets. P. falciparum Hsp70-1 (PfHsp70-1; PF3D7_0818900) is an essential heat shock protein involved in the folding of newly synthesized and misfolded polypeptides and is also implicated in protein trafficking. PfHsp40 (PF3D7_1437900.1) is a type I Hsp40 co-chaperone of PfHsp70-1 …
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Identification of small molecule inhibitors targeting Plasmodium falciparum Hsp70-Hop pathway
… Hop (PfHop; PF3D7_1434300) brings together PfHsp70-1 and PfHsp90 into a functional complex. PfHop interacts with PfHsp70-1 (PF3D7_0818900) and PfHsp90 (PF3D7_0708400) through its tetracopeptide repeats (TPR) domains. PfHsp70-1 and PfHsp90 possess C-terminal EEVD and MEEVD motifs, …
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Elucidation of the role of the linker motifs of Plasmodium falciparum Hsp70-1 and Hsp70-z
… the parasite Hsp70 family members, amongst them, PfHsp70-1 and PfHsp70-z, are essential for parasite survival. PfHsp70-1 is a canonical Hsp70, which closely resembles Escherichia coli Hsp70, DnaK. On the other hand, PfHsp70-z is a non-canonical Hsp70 which belongs to the Hsp110 subfamily. Hsp70s …
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Characterisation of the plasmodium falciparum HSP70-HSP90 organising protein
… linked to its life cycle. P. falciparum Hsp70-1 (PfHsp70-1; PF3D7_0818900) and P. falciparum Hsp90 (PfHsp90; PF3D7_0708400) are some of the major Hsps involved in the cytoprotection of the parasite and therefore pathogenesis of malaria. Hsp70 and Hsp90 are brought into close proximity by …
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Evaluation of the immunomodulatory activity of Plasmodium falciparum hsp70-1
… in the host environment. P. falciparum Hsp70-1 (PfHsp70-1) is a ubiquitous, cytosol-localised Hsp70 that is essential for parasite survival. Apart from their role as molecular chaperones, it is believed that some Hsps of parasitic origin are capable of modulating host immunity through signal …
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Comparative analysis of a chimeric Hsp70 of E. coli and Plasmodium falciparum origin relative to its wild type forms
… of E. coli DnaK NBD and P. falciparum Hsp70-1 (PfHsp70-1) SBD was previously designed. KPf was shown to confer cytoprotection to E. coli DnaK deficient cells that were subjected to heat stress. In this study it was proposed that KPf has an advantage over E. coli DnaK and PfHsp70-1 in its …
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In cellulo and biophysical exploration of the role of GGMP residues of Hsp70
… For example, Plasmodium falciparum Hsp70-1 (PfHsp70-1) which occurs in the cytosol and nucleus harbors seven GGMP repeats on the C terminus upstream of the EEVD motif. The function of GGMP residues of Hsp70 is largely unclear but they were recently reported to be involved in substrate and …
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Characterization of PFF1010c, a type IV Plasmodium fasciparum heat shock protein 40
… direct interaction between PFF1010c and PfHsp70-1 was conducted and no interaction was observed. Malarial parasites were harvested at different stages and total protein was isolated. The expression of PFF1010c was confirmed to occur at the gametocyte stage of the parasite’s development …
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Development of high-throughput screening assays to identify small molecule inhibitors of Plasmodium falciparum Hsp70-1
… of misfolded proteins. The molecular chaperone PfHsp70-1 is essential for cell survival during stress and it is expressed throughout all the blood stages of the parasite. Thus, Hsp70(s) are essential for functionality in the cell and their function has been demonstrated using the complementation …