Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Perturb-seq"”.
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In Situ Perturb-Seq of Transcriptomes and RNA Neural Recordings
… work, we explore the intersection of in situ sequencing, neural recording, and CRISPR screens. An intracellular technology is outlined for encoding neural activity in the form of RNA, theoretically enabling single-cell resolution recording of whole-brain activity. This neural recording system …
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A PERTURB-SEQ SATURATION MUTAGENESIS APPROACH TO DISSECT THE MOLECULAR BASES OF GENETIC DISEASES
The improvement of novel sequencing technologies is quickly transforming the scientific investigation and the therapeutic treatments of rare genetic diseases, which research is hampered by the limited cohort of diagnosed patients and by the difficulty of evaluating the effect of Variants of Unknown …
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Computational Approaches to Delineate Transcriptional and Functional Heterogeneity In Pancreatic Cancer
… fully leverage advancements in next generation sequencing and functional genomics, we have sought to establish computational methodologies to aid in refined target discovery, and to develop a novel platform to comprehensively characterize the transcriptional heterogeneity of PDAC. Specifically, …
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Causal Representation Learning for Predicting Genetic Perturbation Effects on Single Cells
Advances in sequencing technologies have significantly deepened our understanding of gene regulation in cells. Among these, Perturb-seq has emerged as a powerful technique, enabling high-resolution profiling of transcriptomic responses to genetic perturbations at the single-cell level. Such …
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The Malleability of Gene Regulation in Healthy Individuals: Analyzing CRISPR-based Screens with Single-Cell RNA-Sequencing Readout across Genetic Backgrounds
… technological advances in genome engineering, sequencing technology and cell line differentiation can be used to mitigate these challenges. However, these techniques have been limited in scale and do not account for variation in genetic background. In this thesis, I explore four CRISPR-based …