Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Permeabilized"”.
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Role of ROK and PKC in Permeabilized Rabbit Femoral Artery
… this hypothesis, rabbit femoral arteries were permeabilized with 20µg/ml α-toxin and 1% Triton X-100 and subjected to different calcium concentrations in the presence or absence of various ROK inhibitors. For a comparison we also used various PKC and MLCK inhibitors and repeated these …
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The regulation of luteinizing hormone exocytosis in α-toxin permeabilized sheep anterior pituitary cells
… combines prolonged pre-equilibration of the permeabilized cells at 0°C before stimulation with strong Ca²⁺ buffering. Under these conditions an increase in the [Ca²⁺]free stimulated a 15-20 fold increase in LH exocytosis (EC₅₀ pCa 5.5). After 12-15 minutes the rate of exocytosis declined and …
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Effect of Acute Exercise or Fasting on Mitochondrial Function and High Fat Diet-Induced Insulin Resistance
… Red gastrocnemius muscles were harvested and permeabilized fibers prepared for determination of mitochondrial respiratory function and H[subscript]2O[subscript]2 emission. A single 16 h fast significantly (P<0.05) improved insulin sensitivity in rats maintained on a high-fat diet (P<0.05). …
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Nucleocytoplasmic Localization of MAPKs
… and exportins), ERK2 enters the nucleus of permeabilized cells even if these carrier proteins are missing. This is explained by direct binding to proteins in the nuclear pore complex (NPC). Similar to ERK2 targets, NPC proteins also contain Phe-Xxx-Phe (FXF) motifs. My first aim in this …
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Quantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cells
… to pass through intact cell membranes yet enter permeabilized cells. This dissertation investigates the hypothesis that the flow of such molecules may be used to quantify the effects of the electrical stimulus and environmental conditions leading to membrane electroporation. Specifically, a …
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Postnatal Maturation Modulates Cerebrovascular Myogenic Tone
… reliant on myofilament calcium sensitivity, in a permeabilized preparation, to maintain pressure-evoked myogenic constriction as compared to adults. We measured pressure-evoked myogenic tone in endothelial denuded segments of MCA from P14 and adult rats that where permeabilized using (3-escin. The …
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The Binding Properties and Functional Consequences of Ryr2-Cam Interaction
… still unknown. Here we, Using FRET detection and permeabilized myocytes, identified RyR2-bound CaM from other potential targets and revealed that CaM binds to RyR2 with high affinity (Kd =10-20 nM) in myocytes. Therefore physiologically CaM is bound to > 70% of RyR2 monomers and inhibits SR Ca …
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Investigating the possible cytoprotective effects of melatonin isomer against simulated ischemic injury
… blue staining. Mitochondrial respiration in permeabilized H9C2 cells was measured using the Oroboros Instrument, at two different time points: at the end of a 30min pre-treatment with either 75ng/L melatonin or 75mg/L melatonin isomer, or the afore mentioned pre-treatments prior to a 15min …
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Mechanism and Regulation of ERK2 Subcellular Localization
… transmit signals. In vitro studies using a permeabilized cell system indicate that nuclear import of ERK2 is not regulated by soluble transport factors, but requires access to nucleoporins. While this process is not influenced by classical import machinery, it can be modulated by anchoring …
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Identification and functional characterization of the mitochondrial adenine nucleotide carriers of Trypanosoma brucei
… and absence of the inducible protein, using permeabilized cells with digitonin that confirmed the ADP/ATP transport activity of the carrier. For invitro activity assays, the carriers were cloned and expressed in Escherichia coli and Spodoptera frugiperda, solubilised and reconstituted into …
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Functional Analysis of Wild-Type LGN and T450 Mutants
… Western blot and immunocytochemistry of fixed, permeabilized cells using anti-GPSM2 antibody. Furthermore, the T450A and T450D mutants, which mimic the unphosphorylated and phosphorylated forms of LGN respectively, are being generated by PCR and will be sent for sequencing analysis to confirm …
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Evidence for Absence of Latchbridge Formation in Phasic Saphenous Artery
… SA expressed more MLC phosphatase than FA, and permeabilized (β-escin) SA relaxed more rapidly than FA in the presence of MLC kinase blockade, suggesting that MLC phosphatase activity in SA was greater than that in FA. The reduction in MLC phosphorylation in SA was insufficient to account for …
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Targeting and Localization of Alpha-Synuclein in Primary Neurons
… analysis of alpha-syn retention in digitonin-permeabilized cells indicate that a subpopulation of alpha-syn associates with plasma membrane, but contrary to published reports, this plasma membrane component does not have characteristics consistent with lipid rafts.
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Functional responses of cardiac and skeletal muscle mitochondria to short-term obesity: are all obesities the same?
… was used to examine mitochondrial function in permeabilized solei muscle fibers and isolated cardiac and skeletal muscle mitochondria obtained from all study groups. Body mass of obese animals was 30 and 50% greater than respective lean controls in squirrels and mice, respectively. Glucose …
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The effects of different processing parameters (cold soak and percent alcohol (v/v) at dejuicing) on the concentrations of grape glycosides and glycoside fractions and glycosidase activities in selected yeast and lactic acid bacteria
… the whole cell fraction (66%), followed by the permeabilized fraction (35%), and extracellular production (2%). Aureobasidium pullulans was also capable of hydrolyzing grape glycosides.
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Supramolecular Structure-Enabled Delivery of Porphyrin to Glioblastomas Beyond the Blood-Brain Barrier
… for localized tissue delivery across permeabilized vasculature. Pharmacokinetic optimization of pMB composition was conducted by developing and applying a new microbubble radiolabeling strategy. Multimodal (ultrasound, PET, γ-counting and fluorescence) microbubble dissolution, shell …
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Substrate Interaction and Sub-Cellular Localization in Map Kinase Pathways
… not been investigated. We have used an in vitro permeabilized-cell reconstitution assay in HeLa and BJ fibroblast cells to explore the mechanism of GFP-ERK2 export and His6-tagged thiophosphorylated ERK2 import. Our results identify more levels of regulation within this model. The ERK5 pathway is …
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Delivery of molecular-specific optical contrast agents for cancer biomarker detection in live cells and tissues
… specific to hTERT were delivered into live permeabilized cells. The sensitivity of this approach was validated using cell lines that differentially express hTERT and paired clinically normal and abnormal human biopsies. The feasibility of enhancing tissue permeation with Triton-X100 was …
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Effects of Long-Term, High-Altitude Hypoxemia on Fetal Ovine Coronary Arteries
… maximum Ca2+-activated force in B-escin permeabilized left circumflex and left anterior descending preparations. Furthermore, enhancement of Ca2+-activated force by U46619 was attenuated in the high-altitude left circumflex coronary rings. Ca2+ sensitivity of the contractile proteins; …
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