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Showing 1 to 15 of 15 for “"Perlecan."”.
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Έκφραση και ρόλος της aggrecan και perlecan στο πρώιμο έμβρυο
… ύλη περιέχονται πρωτεογλυκάνες, εκ των οποίων οι perlecan και aggrecan είναι από τις πρώτες που εκφράζονται στο πρώιμο έμβρυο. Μελετήσαμε τη χωρο-χρονική κατανομή των perlecan και aggrecan με RT-PCR, ανοσοφθορισμό και ανοσοκατακρήμνιση από το στάδιο Χ (μορίδιο) έως το στάδιο ΗΗ17 (29-32 σωμίτες) …
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The Heparan Sulfate Proteoglycan Perlecan Regulates Axonal and Synaptic Stability
… and have both structural and signaling roles. Perlecan is a secreted ECM-localized HSPG that contributes to tissue integrity and cell-cell communication. In this thesis, I identify a role for Drosophila Perlecan in the maintenance of larval motoneuron axonal and synaptic stability. In Chapter …
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Integrin Distribution in Collagen Iv Mutants and Use of Rna Interference to Identify a Collagen Iv Receptor in C. Elegans
Perlecan, a basement membrane protein, was found to be significantly decreased in collagen IV mutants. To better understand the role of collagen IV in perlecan-integrin dependent sarcomere organization, an extrachromosomal array containing a functional ßpat-3::GFP fusion gene was mated using …
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Tissue-Specific Matrix Control of Cell Cohesion and Migration Signaling Complexes
… complexes (type I hemidesmosomes and the perlecan-semaphorin 3A-plexin A1-neuropilin-1 (PSPN) complex) and translates this knowledge into viable solutions for both tissue engineering and cancer metastasis applications. Database (Matrisome/FANTOM5) and two-dimensional and three-dimensional …
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Characterisation of novel matrix-binding interactions for latent transforming growth factor-β-binding protein-2 (LTBP-2), with emphasis on heparin and heparan sulphate proteoglycans.
… and glypicans and in basement membranes as perlecan. Full length human recombinant LTBP-2 (rLTBP-2) was expressed in 293 EBNA cells using a modified pCEP-4 vector and purified by nickel affinity chromatography. Upon validation of the purified protein using western blots, solid phase binding …
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Nutritional regulation of neural stem cell reactivation in Drosophila melanogaster
… reactivation. Collagen IV recruits glial-derived Perlecan, an- other ECM protein and a known requirement for NSC reactivation, to the vicinity of NSCs. Both ECM proteins are indispensable for the induction of glial insulin/IGF signalling. NSCs are separated from the hemolymph by a blood brain …
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Regulatory roles of endothelial cells in cancer
… cell state - silencing of the gene encoding perlecan - causes loss of the invasion-inhibitory capabilities of endothelial cells by transcriptional upregulation of IL-6. The use of matrixembedded endothelial implants enabled the effects in cell culture to be expanded and validated in animal …
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The extracellular matrix of the nematode Caenorhabditis elegans : a model to study the role of cell-matrix interaction in animal development.
… role in tissue formation. I focused on UNC-52/perlecan, a proteoglycan ECM molecule, because it contains two RGD (Arg-Gly-Asp) motifs in their domains. The mutations in two RGD motifs showed that the removal of a motif in RGD2023 position resulted in embryonic lethality, while a point mutation, …
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Mechanisms of Type IV Collagen Targeting to Developing Basement Membranes
… of the matricellular proteins nidogen, agrin, perlecan, and SPARC in the targeting of collagen to pharyngeal and gonadal BMs. In Chapter 4, I identify potentially novel regulators of type IV collagen incorporation into BMs through a genome-scale RNAi screen. Finally, in Chapter 5, I discuss the …
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Defining the role of heparan sulfate proteoglycans in APL-1 function in Caenorhabditis elegans
… (HSPGs), such as LON-2 glypican and UNC-52 perlecan, are likely candidates for the ECM proteins that interact and guide sAPL-1. The disruption of APL-1 causes phenotypical changes, including in learning, reproduction, and memory; these defects are enhanced when HSPG activities are decreased. …
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Genetic and molecular analysis of mutations in the unc-52 gene of Caenorhabditis elegans, and isolation of a suppressor, sup-38
… to the vertebrate basement membrane component, perlecan. In an effort to examine the role of this basement membrane protein in muscle function, I have isolated intergenic suppressors of the class 1 unc-52 mutants. The suppressors are all alleles of a novel gene, sup-38. Animals homozygous for …
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Tissue repair in lung disorders
… matrix molecules such as collagen, versican, perlecan, biglycan, and decorin. Fibrocytes are recruited from the bone marrow to the site of injury. It is possible that the stromal cell-derived factor-1/CXCL12 builds up in a gradient to recruit CXCR4 expressing fibrocytes, at least in the case …
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Crosstalk between natural killer cells and cancer associated fibroblasts in the tumour microenvironment
… matrix proteins osteopontin, laminin and perlecan, but also the lipid metabolite prostaglandin E2 (PGE2). Blocking CAF-derived PGE2 *in vitro* restored NK cell functions such as tumour killing capacity and granzyme B secretion. *In vivo*, targeting this crosstalk by blocking PGE2 receptors …
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
… PG-II respectively. Western blotting with anti-perlecan primary antibody recognized the core proteins of BAE PG. Size exclusion chromatography (Sepharose CL-6B column) following β-elimination showed that BAE PG had GAG chains with an estimated size less than 2 x 10⁵ Da. A protocol to investigate …
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New Insights into the Regulatory Network Controlling Neuroblast Migration in C. elegans
… migration. Interestingly we identified unc-52/Perlecan to be required for AQR and PQR migration, which is a new phenotype. These data will help gain insight on how ETR-1 acts in Q neuroblast migration, as well as new factors.In chapter IV we detail a new role of the Caenorhabditis elegans …