Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Peripheral membrane protein"”.
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Sorting of Cargo Proteins Within the Regulated Secretory Pathway: the Peripheral Membrane Protein HID-1 as a Sorting and Vesicle Biogenesis Factor
… pathways in real-time and that the bulk of transmembrane LDCV cargoes do not sort directly onto LDCVs, but exit the TGN into non-regulated vesicles to be incorporated to LDCVs at a later step. Additionally, we characterize the TGN peripheral membrane protein, HID-1, as a LDCV biogenesis factor. …
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Biochemical and Cellular Characterization of NMN Adenylyltransferase 2: A Brain Specific Isoform of an Essential NAD Synthesizing Enzyme
… I have discovered that Nmnat2 is a neuronal peripheral membrane protein anchored via palmitoylation that localizes to dendrites and the cell body. Furthermore, I argue that a low level of Nmnat2 in the axon, or possibly its exclusion altogether, is required for normal (i.e. fast) axon …
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Investigating the role of TspA, a novel substrate of the <i>Staphylococcus aureus</i> Type VII protein secretion system
… was used to identify candidate T7 substrate proteins. A previously uncharacterised protein, SAOUHSC_00584, was found in the culture supernatant in a T7-dependent manner. Cell fractionation and protease accessibility experiments demonstrated that SAOUHSC_00584 is a peripheral membrane protein …
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Biochemical characterization of the first step in ether lipid biosynthesis: acylation of dihydroxyacetone phosphate
… in the body and noticeably abundant in membranes of the nervous system. Compared to the four GPATs, DHAPAT is understudied. Current knowledge associating DHAPAT deficiency with many human disorders has re-kindled the interest in the research community. Symptoms of DHAPAT deficiency have …
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Interfacial modulation of protein function explored through atomistic molecular dynamics simulation
… this dissertation, MD is applied to processes in membrane biology and biofuel research. Through the use of the Highly Mobile Membrane Mimetic (HMMM) to accelerate phospholipid motion, we show that phospholipid tails insert significantly faster than they do in a conventional bilayer, and repeated …
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Biophysical Dynamics Of Rgs-Lov Proteins As Systems For Light-Induced Membrane Recruitment
… Recently, we reported BcLOV4, a LOV protein from B. cinerea, which demonstrates a light-induced protein-lipid interaction with the plasma membrane. Previous work demonstrated preliminary characterization of BcLOV4 and its utility as an optogenetic tool. In this thesis, I expand on …
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Quantitative Fluorescence Studies in Living Cells: Extending Fluorescence Fluctuation Spectroscopy to Peripheral Membrane Proteins
The interactions of peripheral membrane proteins with both membrane lipids and proteins are vital for many cellular processes including membrane trafficking, cellular signaling, and cell growth/regulation. Building accurate biophysical models of these processes requires quantitative …
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Computational investigation of membrane proteins across species and functional classes
Membrane proteins play central roles in cellular communication, metabolism, and homeostasis, yet experimental characterization of their dynamic interactions with membranes remains highly challenging. In this dissertation, I implement advanced molecular dynamics (MD) simulation workflows to …