Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Peptidyl-tRNA"”.
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The Impact of Depleting the Essential Bacterial Enzyme Peptidyl-tRNA Hydrolase on Translation
Peptidyl-tRNA hydrolase (Pth) is an essential bacterial enzyme that recycles peptidyl-tRNAs (pep-tRNAs) by hydrolyzing the ester bond between the tRNA and the peptide. The best-characterized function of Pth is salvaging free pep-tRNAs that dissociate from the ribosome during translation to prevent …
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Investigating the Molecular Mechanism of Ribosome Recycling
… recycling. Amikacin interferes with tRNA translocation, release factor-mediated peptidyl-tRNA hydrolysis, and ribosome recycling, traits attributed to the additional interactions amikacin makes with the decoding center. The binding site in the large ribosomal subunit proximal to the …
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Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
… one during translocation from the aminoacyl-tRNA binding (A) site to the peptidyl-tRNA binding (P) site and the second during occupancy in the P site. Using acoustic radiation force, the force resolution was improved to 0.5 pN, sufficient to resolve a single hydrogen bond difference. Taking …
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Studies of Entry, Reverse Transcription, and Regulation of Splicing in Retroviruses
… transcription. Retroviruses use host cell tRNAs which must first be deaminoacylated before the RT enzyme can begin reverse transcription. This work examines the crystal structure of human immunodeficiency virus type one (HIV-1) RT for a possible hydrolase site that is capable of …
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Studies on Ribosome-Membrane Interaction
… ribosomes as unfolded subunits, while the peptidyl tRNA molecules remained associated with the microsomal vesicles. c) An exchange of small subunits between free and membrane-bound ribosomes was found to occur in vitro upon release of polypeptide chains under conditions thought to …
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Genetic decoding dynamics: from its exploitation to enhance or rescue gene expression to a search for relevance for early evolution
… provided strong evidence that the efficiency of peptidyl-tRNA dissociation from the take-off codon at the start of the coding gap is 100%. Therefore, the ability of ribosomes to recognise the glycine landing codon and resume translation is key for efficient bypassing. The identity of the matching …
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Ανάπτυξη και χαρακτηρισμός νέων αντιβιοτικών - αναστολέων της πρωτεϊνικής σύνθεσης
… τα οποία προσομοιάζουν πεπτιδυλο-tRNAs προσδεδεμένα στην Α-θέση του ριβοσώματος, με την ολιγοπεπτιδική αλληλουχία να εκτείνεται βαθύτερα στο τούνελ, υιοθετώντας μία ανοικτή διαμόρφωση. Όπως διαπιστώσαμε με συναγωνιστικές μελέτες με ραδιενεργό ερυθρομυκίνη, τα συγκεκριμένα ανάλογα …