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Showing 1 to 20 of 22 for “"Peptide transporter"”.
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Cloning, Expression, and Developmental and Dietary Regulations of a Chicken Intestinal Peptide Transporter and Characterization and Regulation of an Ovine Gastrointestinal Peptide Transporter Expressed in a Mammalian Cell Line
To study peptide absorption in chickens, an intestinal peptide transporter cDNA (cPepT1) was isolated from a chicken cDNA library. The cDNA was 2,914-bp and encoded a protein of 714 amino acid residues. Twenty-three di-, tri-, and tetra-peptides were used for functional analysis of cPepT1 in …
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Tissue Distribution of a Peptide Transporter mRNA in Sheep, Dairy Cows, Pigs, and Chickens
… found in sheep omasal epithelium encoding for a peptide transport protein(s), a 446-bp cDNA fragment was cloned from sheep omasal epithelium RNA. The predicted amino acid sequence of this fragment was 85.8, 90.5, and 90.5 percent identical to rabbit, human, and rat PepT1, respectively. The …
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Cloning, Sequencing and Expression of a Porcine Intestinal Peptide Transporter in a Mammalian Cell Line
… the uptake of free amino acids or as small peptides. A peptide transport protein, PepT1, is responsible for the absorption of intact peptides arising from digestion of dietary proteins. PepT1 is driven by a H+-coupled transport system that allows for the absorption of small peptides through …
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Molecular Cloning, In Vitro Expression, and Functional Characterization of an Ovine Gastrointestinal Peptide Transporter (oPepT1)
… and in vitro functional characterization of a peptide transporter, oPepT1, from ovine intestine. The ovine intestinal oPepT1 cDNA was 2,829 bp long encoding a protein of 707 amino acid residues with an estimated molecular size of 79 kDa, and a pI of 6.57. The cDNA contained a 79-bp 5' …
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Gene Expression of the Intestinal Peptide Transporter 1 and Peroxisome Proliferator-Activated Receptor alpha Following Fasting/Refeeding and Ligand Administration in Chickens
… uptake of amino acids is mediated by amino acid transporters and the peptide transporter, PepT1. The nuclear receptor PPARα may play a role in the activation of PepT1. The objectives of this study were to evaluate the effect of a fasting/refeeding regimen and gavaging a PPARα ligand on …
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Molecular Cloning and Functional Characterization of a Turkey Intestinal Peptide Transporter (tPepT1), and Developmental Regulation of PepT1 Expression in Turkey and Broiler Embryos
A cDNA clone encoding a turkey intestinal peptide transporter, tPepT1, was isolated from a turkey small intestinal cDNA library by screening with our chicken PepT1 (cPepT1) cDNA probe. The tPepT1 cDNA is 2,921-bp long and encodes a 79.4 kDa protein of 714 amino acids (AA) with 12 predicted …
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Poly(A)+ RNA From Sheep Omasal Epithelium Induces Expression of a peptide Transport Protein(S) in Xenopus laevis Oocytes
… omasal epithelium contains mRNA encoding for a peptide transporter (s) and to determine di- to octapeptide transport capability, poly(A)+ RNA isolated from sheep omasal epithelium was injected into Xenopus laevis oocytes. Poly(A)+ RNA was functionally expressed in Xenopus oocytes 4 to 7 d …
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Gene Expression of Nutrient Transporters and Digestive Enzymes in the Yolk Sac Membrane and Small Intestine of the Developing Embryonic Chick
… mediated by digestive enzymes and membrane bound transporter proteins. The objective of this study was to determine expression profiles of nutrient transporters and digestive enzymes during incubation in the yolk sac membrane (YSM) and small intestine of Leghorn and Cobb chickens derived from …
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Delayed access to feed affects broiler small intestinal morphology and intestinal cell ontogeny
… Olfactomedin 4 (Olfm4), Marker of Ki-67 (Ki-67), Peptide Transporter 1 (PepT1), and Mucin 2 (Muc2), in response to DAF. The Olfm4 mRNA can clearly identify stem cells in the intestinal crypt, which allows VH and CD to be measured, while Ki-67 marks the proliferating cells. The peptide transporter …
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Developmental Regulation of the Expression of Nutrient Transporter and BrushBorder Membrane Hydrolase Genes in the Small Intestine of Piglets
… regulation of the expression of nutrient transporter and brushborder hydrolase genes in the small intestine of piglets. Seventy piglets from seven sows were killed at birth (d 0), during suckling (d 1, 3, 7, 14, 21) and postweaning (d 22, 24, 28, 35), and intestinal segments (duodenum, …
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Ocular pharmacokinetics and efficacy of various amino acid and dipeptide prodrugs of ganciclovir
… ocular bio-availability of GCV. A series of dipeptide monoester prodrugs and amino acid monoester and diester prodrugs were designed to target the nutrient transporters peptide transporter (hPEPT1) and sodium dependent neutral and cationic transporter (B [super 0,+]), respectively. The overall …
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Short-Chain Fatty Acids and Functional Adaptations in the Neonatal Intestine
… SCFA and Bu would enhance glucose transport and transporter gene expression in a neonatal pig model of massive small bowel resection. Two-day-old neonatal pigs underwent 80% jejunoileal massive small bowel resection, and were fed via total parenteral nutrition (TPN). Piglets were randomized to …
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Computational methods to design biophysical experiments for the study of protein dynamics
… are performed to study a bacterial di/tri-peptide transporter a typical representative of the Major Facilitator Superfamily of membrane proteins. This was followed by ideal double electron-electron resonance experimental choice predictions based on the simulation data. The predicted choices …
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Regulatory Balance Between the Peptide Trasporter, Pept1, and Amino Acid Transporter Gene Expression in the Enterocyte
… acids are assimilated by membrane-associated transporters into and out of enterocytes either in their free form or in the form of peptides. The peptide transporter, PepT1, is thought to be the major facilitator of peptide transport in the enterocyte. It is unknown if the peptide transporters …
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Effects of high incubation temperature on the developing small intestine and yolk sac of broiler chicks with insight into goblet cell development in the small intestine early posthatch
… higher temperature showed greater expression of peptide transporter 1 and avian beta-defensin 10 mRNA at embryonic day 13. Even though small intestinal morphology was impacted early posthatch and expression of genes in the yolk sac were elevated at embryonic day 13, there does not seem to be a …
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Sustained release nanoparticles containing acyclovir prodrugs for ocular herpes simplex keratitis and characterization of folate transport proteins in a corneal epithelial cell line
… polymeric nanoparticles of stereoisomeric di-peptide prodrugs of acyclovir (L-valine- L-valine-ACV, L-valine-D-valine-ACV, D-valine-L-valine-ACV, and D-valine-D-valine-ACV) were developed and characterized for the treatment of ocular herpes keratitis. L-valine-Lvaline- ACV and …
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Distribution and Relative Abundance of Nutrient Transporter mRNA in the Gastrointestinal Tract of Black Bears
… are absorbed by the body through the action of transporter proteins expressed on the brushborder membrane of small intestinal epithelial cells. The goal of this study was to increase the understanding of the physiological processes associated with nutrient assimilation by black bears. …
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Functional characterization of nitrate transporters in maize.
… by nitrate permeable transport proteins. Nitrate transporters have been extensively studied in the model plant, Arabidopsis thaliana. Nitrate transporters belong to three protein families: NPF (Nitrate Transporter 1/Peptide Transporter), NRT2 (Nitrate Transporter 2) and CLC (Chloride Channel) …
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Outlining a balance-point model of homeostasis in the small intestine of broiler chickens
… gene expression of multiple host defense peptide genes were decreased compared to uninfected chickens. Further, Eimeria infected chickens increased cell proliferation within the crypt and post-peak infection showed signs of intestinal recovery. Additionally, chapter 3 developed a novel …
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The Influence of Necrotic Enteritis, Environmental Factors, and Genetics on Intestinal Development Pathways and Disease Occurrence in Broiler Chickens
… differences between lines were an increase in peptide transporter PepT1 on d5 and d7 in chicks selected for low eight-week body weight (LWS) compared to high weight selected (HWS) chicks and modern broiler Cobb500 chicks. In HWS chicks, the opposite mechanism was observed with an increase in …
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