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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 103 for “"Peptide sequences"”.
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Characterization of the Glycosylation and Signal Peptide Sequences Within the Vaccinia Virus M2 Protein and Influence on ERK1/2 and Nf-Kappab Activation
This dissertation elaborates on M2's molecular functioning as a viral immunomodulator to inhibit both the ERK1/2 and NF-kappaB pathways, which is reliant on its glycosylation status and proper cellular trafficking within the infected cell. As M2 has no homology to any known viral or cellular …
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Characterization of the glycosylation and signal peptide sequences within the vaccinia virus m2 protein and influence on ERK1/2 and NF-kB activation
… acid sequence revealed an N-terminal signal peptide sequence, 4 putative glycosylation residues, and endoplasmic reticulum (ER) retention/retrieval sequences, indicative of an ER-localized protein. Confocal microscopy analysis confirmed localization of M2 to the ER. It was found that M2 …
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Developing Low Fouling Peptides and Zwitterionic Materials for Biomedical Applications
… prevent biofouling in biomedical applications. Peptides are considered for use as natural low fouling materials due to their biocompatibility, diverse chemical properties, well-defined sequence, and biodegradability. Low fouling peptide sequences were rationally designed from known zwitterionic …
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A study of peptide affinity for carbon nanotubes through phage display experiments
… activity. In this work we sought to identify peptide sequences with selective affinity for HiPco-produced SWNTs in order to gain some insight to the binding mechanisms and interactions. This was done using a phage display technique, in which a library of bacteriophages displaying greater than …
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From Prohormones to Neuropeptides: Mass Spectrometric and Bioinformatic Approaches
… is being sequenced. After deducing the neuropeptide genes through bioinformatic and mass spectrometric approaches, the neuropeptides are predicted with the Aplysia-based algorithms. To verify all predictions, the peptide sequences are confirmed by electrospray quadrupole mass spectrometry …
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Funktionalisierung von Polydimethylsiloxan-Oberflächen zur Steuerung molekularer Zell-Substrat Wechselwirkungen
… to amino groups, produced in the PDMS surface. Peptide sequences or aptamers were reacted with the hydrogel layer to allow specific cell adhesion via ligand-receptor interactions. First PDMS surfaces were functionalised with amino groups. Exposition in ammonia-plasma leads to higher surface …
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Conjugation of Zwitterionic and Cationic Peptides to Lipid and Polymer for Gene Delivery
… by the chemical conjugation between the peptides and the corresponding backbones. In total, we focus on 3 types of conjugates mainly. They are the zwitterionic peptide (EK)x pair lipid DSPE conjugates, THE DSPE-(EK)x, the cationic ionizable peptide RxHy lipid DSPE conjugates, the …
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Reactive Peptides for Site-Selective Cysteine and Lysine Bioconjugation
… address these challenges, we developed a matched peptide pair termed the reactive peptide interface (RPI). This interface consists of two peptides: a nucleophilic, cysteine-containing peptide and an electrophilic, perfluoroarylated peptide. The unique sequences of these peptides enhance the …
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Active Insulin Analogs That Do Not Require Refrigeration
… thus do not require refrigeration. Solid phase peptide synthesis techniques and the incorporation of backbone modified amino acids into peptide sequences will be used to design novel insulin analogs.
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Characterization of the primary structure of a size-variant antifreeze peptide and the organization and structure of antifreeze peptide genes from the Antarctic eel pout Rhigophila dearborni
… dearborni synthesizes three major antifreeze peptides (AFPs) which have been designated RD1, RD2 and RD3, and at least four minor ones. RD1 and RD2 both are 64 residues with a molecular weight (M.W.) of about 7000 Daltons. RD3 however appears to be twice as large with a M.W. of 14,000 Daltons. …
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Development of a Passive Immunization Strategy Against Atherosclerosis
… responses. These epitopes are aldehyde-modified peptide sequences of apoB-100, the main protein in LDL structure. Immunization of atherosclerosis-prone mice with some of the apoB-100 peptides reduced plaque area by up to 60% in the immunized mice compared to controls. We tested the effects of …
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The design, synthesis and evaluation of selective, non invasive imaging agents for atherosclerotic plaque.
… via MRI. In order to achieve this, a library of peptide substrates designed to be substrate specific for either legumain or MMP-2/9 were synthesized. This was achieved via a combination of both solid and solution phase peptide synthesis. The first phase of the research study focused on the …
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Design of Nuclear-Targeting Peptides for Macromolecule Delivery via Machine Learning
The effective design of functional peptide sequences remains a fundamental challenge in biomedicine. For example, cell-penetrating peptides (CPPs) are capable of delivering macromolecular cargo to intracellular targets that are otherwise inaccessible. However, design of novel CPPs with high …
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A Nascent Peptide Code for Translational Control of mRNA Stability in Human Cells
… cells. We identify several families of simple dipeptide repeats whose translation triggers mRNA destabilization. Rather than individual amino acids, specific combinations of bulky and positively charged amino acids are critical for the destabilizing effects of dipeptide repeats. Remarkably, …
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Evaluation and assignment of significance levels to peptide identifications from the database search programs using resampling approach
… testing that improves the calculation of the peptide match significance levels and detection rate in database search programs is demonstrated. Novel k-permuted decoy databases (where k denotes the type and number of permutations) were evaluated for accurate computation of match significance …
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Metallacyclopeptide
Therefore, linear peptides with two metal binding sites were obtained by solid phase synthesis. Upon coordination of these ligands to a metal centre a metalla-cyclopeptide will be formed. The choosen peptide sequences are found in naturally occuring proteins or cyclopeptides, which bioactivities …
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Optimized GeLC-MS/MS for Bottom-Up Proteomics
… by MALDI TOF mass spectrometry using 18O-labeled peptides as internal standards as well as by label-free quantification approach, which utilizes intensities of peptide ions detected by nanoLC-MS/MS. In the performed kinetic study the effect of temperature, enzyme concentration and digestion time …
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Biologically inspired materials for electro-responsive coatings and the photo-oxidation of water
… have enabled the combinatorial screening of peptides against a multitude of materials to which natural systems typically have no exposure. These techniques have yielded peptides that can bind and assemble technologically relevant materials such as gold and CdS. In this work, combinatorial …
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