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Showing 1 to 5 of 5 for “"Peptide separation"”.
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Characterisation of reversed phase chromatography peptide separation systems
… imperative to have a firm understanding of the peptide separation system. The chromatographic profile can be extremely complex for peptides with various impurities or degradation products, thus it is essential to be able to maximise selectivity differences to identify each species. This thesis …
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CE-microreactor-CE-MS-MS for protein analysis
… a pepsin-immobilized microreactor, separate peptides in a second dimension, and detect the peptides with MS. The development begins from the MS end by coupling capillary free zone electrophoresis (CZE) to a linear ion trap mass spectrometer with a sheath flow interface, and works its way …
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Protein LC-MS Using Slip Flow Chromatography
… Due to the lack of resolution on intact histone separations, conventional methods for histone analysis require time-consuming digestion, which often leads to the loss of PTM information. Slip flow chromatography with orderly packed nonporous silica particles has been shown to greatly increase the …
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Cucurbit[n]uril-engineered Nano-constructs for Molecular Sensing
… of the prepared nanostructures such as peptide separation and high-performance catalysis are also discussed. In the first chapter, the historical development and the remaining challenges in the field of SERS are discussed. Three types of the most commonly used SERS substrates are …
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Supercritical Fluid Chromatography of Ionic Compounds
… behavior. Lastly, we successfully eluted large peptides (up to 40 mers) containing a variety of acidic and basic residues in SFC. We used trifluoroacetic acid as additive in a CO2/methanol mobile phase to suppress deprotonation of peptide carboxylic acid groups and to protonate peptide amino …