Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Peptide Transport"”.
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Ontogenesis of Peptide Transport and Morphological Changes in the Ovine Gastrointestinal Tract
… between age and diet on the potential for peptide transport via PepT1 in the gastrointestinal tract of lambs and to relate changes of peptide transport capability to morphological changes. A 2x4 factorial arrangement of treatments was used with 32 crossbred lambs. Four blocks were created …
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Poly(A)+ RNA From Sheep Omasal Epithelium Induces Expression of a peptide Transport Protein(S) in Xenopus laevis Oocytes
… omasal epithelium contains mRNA encoding for a peptide transporter (s) and to determine di- to octapeptide transport capability, poly(A)+ RNA isolated from sheep omasal epithelium was injected into Xenopus laevis oocytes. Poly(A)+ RNA was functionally expressed in Xenopus oocytes 4 to 7 d …
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Cloning, Sequencing and Expression of a Porcine Intestinal Peptide Transporter in a Mammalian Cell Line
… the uptake of free amino acids or as small peptides. A peptide transport protein, PepT1, is responsible for the absorption of intact peptides arising from digestion of dietary proteins. PepT1 is driven by a H+-coupled transport system that allows for the absorption of small peptides through …
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Tissue Distribution of a Peptide Transporter mRNA in Sheep, Dairy Cows, Pigs, and Chickens
… found in sheep omasal epithelium encoding for a peptide transport protein(s), a 446-bp cDNA fragment was cloned from sheep omasal epithelium RNA. The predicted amino acid sequence of this fragment was 85.8, 90.5, and 90.5 percent identical to rabbit, human, and rat PepT1, respectively. The …
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Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles
In order to characterize peptide transport in the ovine small intestine, [14C]-glycyl-sarcosine uptake by tissue collected from five sheep was studied through the use of brush border membrane vesicles (BBMV). Preliminary experiments determined that incubation in hyaluronidase is not necessary in …
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The cystic fibrosis transmembrane conductance regulator and acid-base transporters of the murine duodenum
… isozyme of the small intestine, during H⁺/peptide transport to minimize intracellular acidification and sustain nutrient absorption.
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Regulatory Balance Between the Peptide Trasporter, Pept1, and Amino Acid Transporter Gene Expression in the Enterocyte
… acids are assimilated by membrane-associated transporters into and out of enterocytes either in their free form or in the form of peptides. The peptide transporter, PepT1, is thought to be the major facilitator of peptide transport in the enterocyte. It is unknown if the peptide transporters …
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Cloning, Expression, and Developmental and Dietary Regulations of a Chicken Intestinal Peptide Transporter and Characterization and Regulation of an Ovine Gastrointestinal Peptide Transporter Expressed in a Mammalian Cell Line
To study peptide absorption in chickens, an intestinal peptide transporter cDNA (cPepT1) was isolated from a chicken cDNA library. The cDNA was 2,914-bp and encoded a protein of 714 amino acid residues. Twenty-three di-, tri-, and tetra-peptides were used for functional analysis of cPepT1 in …
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The proton-coupled oligopeptide transporters PEPT2, PHT1 and PHT2 mediate the uptake of carnosine in glioblastoma cells
… we investigated which proton-coupled oligopeptide transporters (POTs) are present in glioblastoma cells and how they contribute to the uptake of carnosine. Therefore, mRNA expression of the four known POTs (PEPT1, PEPT2, PHT1, and PHT2) was examined in three glioblastoma cell lines, ten …
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Understanding antigen processing in chickens using genome editing technology
… surface glycoproteins that present antigenic peptides to T lymphocytes of the immune system. Several other molecules are involved in loading and optimising the peptides for the two major groups of classical MHC molecules, including the transporter associated with antigen processing (TAP) for …
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Structure-function studies of SLC15 peptide transporters
The proton coupled oligopeptide transporter (POT) family of proteins includes mammalian PepT1 (SLC15A1; high capacity, low affinity) and PepT2 (SLC15A2; low capacity, high affinity) transporters which are involved in the co-transport of protons and di- and tri- peptides and drugs similar in …
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Development of in vitro infant intestinal models to assess responses to infant formulae
… NaB uptake occurred via monocarboxylate transporters (MCT-1, SMCT-1). However, NaB caused elevated apoptosis (Caspase-3 activity), stress responses (p21, HSP-70), and cytokine secretion (IL6, IL8, TNF). Therefore, NaB disturbed barrier integrity, and this disruption was not reversible …