Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 34 for “"Peptide Fragments"”.
-
Nanoparticle-chaperoned urinary 'synthetic biomarkers' for profiling proteases in cancer
… thesis, work is presented on long circulating peptide-nanoparticle probes that can survey, sense, and remotely report on dysregulated protease activities in cancer. In this strategy, iron oxide nanoparticles are utilized as chaperons to deliver protease-specific peptide libraries to tumors …
-
Computationally Designed Peptide Binder and Molecular Beacon for SARS-CoV-2
… validation to develop an ACE2-derived peptide that targets the viral spike protein receptor-binding domain (RBD). The peptide was derived utilizing the existing crystal structure of spike protein’s RBD and ACE2 to determine the linear peptide fragments that contributed the most to the …
-
Molecular identification of silk proteins in the gumfooted lines and attachment discs of the black widow spider, latrodectus hesperus
… fibroins of the gumfooted lines.</p> <p>Novel peptide fragments from solubilized gumfooted lines were acquired from manual de novo MSIMS sequencing after in-gel tryptic digestion. These peptide fragments showed post-translational modifications consistent with glycosylation, which aligns with …
-
Bacterial Luciferase: Studies of Proteolytic Inactivation and Ligand Binding
… of the (alpha) subunit into a discrete series of peptides, denoted the (gamma) and (delta) families. The region of the (alpha) subunit which was susceptible to proteolytic clipping was termed the Proteinase Labile Region and was observed to be a conserved structural feature in luciferases from …
-
Analysis of Peptide Quantification and Digestion through Assays, HPLC, and Modification of Workflow
… a believable and accurate method to quantify peptides, by using a variety of commercial protein assays and HPLC. In the field of proteomics, mass spectrometry is the premier method for attaining the mass to charge ratio of a protein. Proteins must be applied to the mass spectrometer as …
-
Protein Three -Dimensional Structure: Theoretical and Computational Aspects of Structure Prediction Based on the Amino Acid Sequence
… Towards this end, we created libraries of peptide fragments of sizes 7 to 13 respectively and clustered them based on structural similarity. Analysis of these clustered fragments show that these adequately describe the protein structure space. In order to use this paradigm for ab initio …
-
Taraxacum officinale as an expression system for recombinant proteins : molecular cloning and functional analysis of the genes encoding the major latex proteins
… officinale Web., contains mainly four abundant peptides with an apparent molecular mass of 66, 60, 37, 18 kDa. These four peptides were termed major latex protein (MLPs). The identification of the MLPs and cloning of their corresponding genes were undertaken with the objective to isolate useful …
-
A Biochemical Study of Gossypol and Lactate Dehydrogenase X Binary Interactions
… (HPLC) separation of the LD-X tryptic peptide fragments to observed alterations in separation patterns as indicators of intramolecular disturbance in the enzyme molecule. Definite alterations in peptide fragment peaks were observed in the presence of gossypol and suggest conformational …
-
Structure-guided Cancer Vaccines based on Glycopeptides Supported on Diverse Nanoparticles
… cancer vaccines, which can use tumor-associated peptide fragments that can induce an immune response. In this thesis, we focus on the peptide sequence of MUC1, a glycoprotein presents on the surface of epithelial cells, which leaves simple carbohydrates, and the backbone of the peptide exposed in …
-
The development of a method to deliver neuroprotective peptides specifically into stroke-affected neurons
… into the neuroprotective Tat-GluR2/3Y peptide. Although in vivo detection of modified Tat-GluR2/3Y peptides was unsuccessful due to technical difficulties, the accumulation of the therapeutic 3Y peptide fragments in neurons under excitotoxic conditions in vitro was found to increase …
-
Combined experimental and modeling studies reveal new mechanisms in T cell antigen recognition
… of the immune system recognize small antigen peptide fragments loaded onto Major Histocompatibility Complex (pMHC) molecules through their T Cell Receptor (TCR). The recognition of antigenic pMHC by the TCR is an extremely sensitive and specific process, discriminating as few as a single …
-
Total Synthesis of Himastatin
… on expedient preparation of a macrocyclic depsipeptide monomer via hybrid solution/solid phase peptide synthesis, followed by a newly developed oxidative dimerization reaction to secure the C5–C5' biaryl linkage at the center of himastatin’s homodimeric structure. Application of the oxidative …
-
Effects of Extracellular Polymeric Substance Composition on Bacteria Disinfection by Monochloramine
… The analyses results suggested the reactions of peptide fragments of proteins that are associated with previously recognized cellular processes (Glycogen Synthase, D-ribose pyranase, Glycerol-3-phosphate acyltransferase, and Glucose-6-phosphate 1 dehydogenase). In addition, the impact of the …
-
A T cell receptor transgenic approach to the study of thymocyte development and autoimmune disease
CDS+ T lymphocytes recognize short peptide fragments when bound to self-class I major histocompatibility proteins. On a mature lymphocyte, such recognition results in activation of the T cell. On an immature thymocyte, such recognition may result in either positive selection, allowing the cell to …
-
Investigating the Immunological Effect of Schizophrenia-Related Antigens and Anti-psychotic Medication.
… to investigate the immune response to linear peptide fragments derived from 15 distinct proteins encoded by schizophrenia-related genes. An antibody test was developed in-house to detect plasma IgG antibodies against selected target molecules in patients with schizophrenia and control …
-
The amino acid sequence of the major alpha globin of the rat (Wistar, Rattus norvegicus)
… substantially determined. The soluble tryptic peptides prepared from the native ∝ globin and from the aminoethylated ∝ globin were separated by peptide mapping. Sequencing of the tryptic peptides was carried out by the dansyl-Edman method and by the overlapping of smaller peptide fragments …
-
Approaches to Study Small Molecule Inhibitors and Their Targets
… signature for identifying inhibitor-modified peptide fragments in complex mass spectrometry data. I applied this approach to an ATP-competitive inhibitor of kinesin-5, a widely conserved motor protein required for cell division. This analysis, along with mutagenesis studies, suggests that the …
-
Tool Development for Studying and Manipulating Peptide-MHC Interactions in a Globally-Representative Manner
… role in the adaptive immune system, presenting peptide fragments on the surface of cells for surveillance by T cells. In this way, T cells are able to sense cellular dysfunction associated with disease, such as the presence of pathogen-derived peptides. The ability to assess and predict …
-
Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity
… T cell receptor that are specific for SIINFEKL peptide (OVA 257-264) in association with class I MHC molecules. The K14-SOVA/OT-1 (#5 and #17) DTg mice develop normally, except that they undergo a destructive process that selectively targets the external pinnae in the first 6 days of life. The …
-
Development of Non-Atp Competitive, Plk1-Selective Inhibitors
… REPLACE uses structure activity relationships of peptide inhibitors to generate a pharmaceutically acceptable lead molecule by replacing individual amino acid residues with non-peptide fragments. REPLACE was used to identify fragment mimetics for determinants from the endogenous phosphopeptide …
Page 1 of 2