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Showing 1 to 14 of 14 for “"Pectate Lyase"”.
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Evolution of a gene for pathogenicity: endo-pectate lyase
… from a. previously isolated extracellular endo-pectate-pectate lyase gene from Ecc; one pectolytic clone had homology to a previously isolated clone encoding endo-polygalacturonase: three clones showed no relationship to either of the previously characterized Ecc pectolytic enzyme genes. A clone …
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Identification of grapevine Pectate Lyase genes altering Berry Texture during ripening
… by polygalacturonases (PGs) and/or cleaved by pectate lyases (PLs), leading to pectin degradation and tissue softening. Previous studies have investigated the role of specific genes or gene families in regulating fruit firmness, particularly pectate lyases. For instance, a knock-out study in …
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Energetic and kinetic folding mechanisms of the parallel β-helix protein pectate lyase C
Pectate lyase C (peIC) was the first protein in which the parallel β-helix structure was recognized. The unique features of parallel β-helix-containing proteins - a simple topology and unusual interactions among side-chains - make peIC an interesting protein to study with respect to protein …
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Molecular mechanisms of pathogenesis incited by Erwinia carotovora subsp. carotovora
… DNA fragment and mediates the production of endo-pectate lyases with isoelectric points (pI) of 9.5 and 7.5 in strain HB101. The pI 9.5 enzyme is believed to be the major extracellular pectolytic enzyme in soft-rot while the pI 7.5 enzyme has no documented counterpart in EC14. Subclone and …
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Transfer of plasmids by genetically-engineered Erwinia carotovora
… chromosomal insertion of a disarmed <i>endo</i>-pectate lyase gene marked with a 1.4kb DNA fragment conferring kanamycin resistance. Plasmids RPI and pBR322 were introduced separately into engineered Ecc clones. These strains served as donors in genetic transfer experiments. No transfer of the …
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Isolation and partial characterization of a water stress protein of the desiccation-tolerant cyanobacterium Nostoc commune UTEX 584 expressed in Escherichia coli
… to have amino acid sequence homologies with a pectate lyase (E 4.2.2.2) periplasmic precursor from <i>Erwinia caratovora</i> subspecies and a dextransucrase (EC 2.4.1.5) precursor from <i>Streptoccocus mutans</i> GS-5. However, pectate lyase activity was not detected in cellular extracts over a …
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PCP and conformational motifs: applications in predicting mechanical strength, allergenicity of proteins and vaccine design
… validation on peanut allergens, the family of pectate lyase showed its ability to distinguish allergenic sequences from non-allergenic ones. In addition, I identified conserved antibody binding epitopes on the envelope E2 proteins of diverse encephalitic alphaviruses and designed hybrid …
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Evaluating effects of climate variability on postharvest quality of strawberries
… contribute to this phenomenon. Contribution of pectate lyase (PEL) action to softening was assessed. No evidence was found to indicate that PEL activity was the key factor for lowering strawberry firmness. Furthermore, plants were cultivated under controlled conditions in growth cabinets in …
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New Pleiotropic Co-Regulators of Gas Vesicle and Secondary Metabolite Production in Serratia sp. ATCC39006
… reduction was observed in carbapenem, cellulase, pectate lyase, N-BHL and siderophore production, and motility. The orf9 gene also had multiple physiological effects in S39006. Reduced GV, prodigiosin and cellulase production, but increased swimming and swarming motilities, were observed in the …
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The role of post-transcriptional regulators in pathogenesis and secondary metabolite production in Serratia sp. ATCC 39006.
… and the plant cell wall degrading exoenzymes, pectate lyase and cellulase. A complex regulatory network controls production of prodigiosin, including a quorum sensing (QS) system, and the role of post-transcriptional regulation was investigated. It was hypothesized that Hfq-dependent small …
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Novel pleiotropic regulators of flotation, secondary metabolite production, and virulence in Serratia sp. ATCC 39006
… The rpoN mutant showed a reduction in GV and pectate lyase production, swimming and swarming motility, and an increase in carbapenem production. The dksA mutant showed a decrease in GV and antibiotic production, motility, and virulence in C. elegans. A quantitative proteomic analysis was …
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Gene regulation in a pathogen-plant interaction: soft rot erwinias versus potato tubers
… high levels and were induced sequentially: exo-pectate lyase (PL), endo-PL, and then endopolygalacturonase (PG) with maximal mRNA accumulations at 6, 9, and 12 hr, respectively. Induction of these mRNAs was well correlated with tissue maceration. In the incompatible interaction, however, …
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Die Strukturbildung der beta-Helix in der Pektatlyase Pel-15
Pektatlyase (Pel-15) aus dem alkalophilen Bodenbakterium Bacillus spec. KSM-P15 ist mit 197 Aminosäuren eines der kleinsten, bekannten β-3-Solenoidproteine. Sie spaltet Polygalakturonsäurederivate in einem Ca2+-abhängigen β-Eliminierungsprozess. Wie bei allen Proteinen dieser Enzymfamilie ist auch …
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Die parallele beta-Helix der Pektat-Lyase aus Bacillus subtilis : Stabilität, Faltungsmechanismus und Faltungsmutanten
Die Pektat-Lyasen gehören zu einer Proteinfamilie, die meistens von pflanzenpathogenen Mikroorganismen sekretiert werden. Die Enzyme katalysieren den Abbau von Polygalakturonsäure, einem Hauptbestandteil in pflanzlichen Mittellamellen und Primärzellwänden. Der Abbau der alpha-1,4-verbrückten …