Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Pdx1"”.
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Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
… overexpression of the transcription factor PDX1 is sufficient to induce β-cell replication, but underlying mechanisms remain to be resolved. Using statistical modeling, we identified the miR-17 family, a member of the miR17~92 miRNA cluster, as a candidate regulator of the PDX1-gene network. …
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Die Regulation der Pankreasentwicklung von Xenopus laevis durch Transkriptionsfaktornetzwerke
… Expression der beiden Transkriptionfaktoren Pdx1/ XlHbox8 and Ptf1a/Xp48 im entodermalen Keimblatt ausreichend sind, um eine Vorlaüferzelle zu einer pankreasspezifischen Zelle zu programmieren. Es bleibt jedoch ungewiss wie frühe RA abhängige Musterbildungsprozesse für die spätere …
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LINEAGE TRACING ANALYSIS OF PANCREATIC ACINAR CELL DE DIFFERENTIATION AND TRANS-DIFFERENTIATION IN VITRO AND IN VIVO IN THE ABSENCE OF MIST 1
… pattern of markers for progenitor cells (PDX1), acinar cells (amylase) and duct cells (CK20) from days 1-11 of culture was examined. Immunofluorescence was performed on cryo- sectioned cultures. Acute pancreatitis was achieved by 7 hourly injections of cerulein (50 pg/kg) and mice were …
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Elucidating the Role of Cellular Architecture in the Developing Pancreas
… in the field, we proceeded to characterize the Pdx1-/- embryo, a mutant in which pancreatic cell fate and architecture had been reported to fail early in its development, to determine whether the developmental failure was related to defects in the epithelial architecture. After elucidating that …
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The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring
… signaling and transcription factors including Pdx1, MafB, IR?, insulin and glucagon. Other pancreata were immunostained followed by image analysis of these factors. Results: In 40F neonates, Ins1, Ins2, glucagon, MafB and IRS2 mRNA expression was reduced. Further, in 30F neonates, Ins1, Pdx1 …
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Struktur- und Funktionsanalysen des Pax4-Promotors
… beeinflussenden Transkrip tionsfaktoren Pdx1, NeuroD und ngn3. Entfernen der einzelnen Motive hat eine Beeinträchtigung der E xpression in der Bauchspeicheldrüse zur Folge.
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FGF signaling in specification of hESC-derived definitive endoderm
… first time to our knowledge that induction of PDX1+ pancreatic progenitors in part relies on 8 FGF2-mediated activation of the MAPK signaling pathway. In the second study, we show that FGF4 alone is not sufficient for induction of foregut endoderm but that in combination with RA it efficiently …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… to direct differentiation of hESCs towards PDX1+ foregut endoderm. The rationale for this was that both RA and FGF signaling exhibit a patterning effect during endoderm patterning and also supports pancreas specification. By optimizing the timing and concentration of RA and FGF4, it was …
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The role of N-cadherin and S1P/S1P1 in pancreas development
… pancreatic bud due to decreased proliferation of Pdx1+ cells. Other epithelial organs revealed growth defects. The lung epithelium had fewer branches and the stomach was smaller. This suggests that endothelial cells are essential for early development of the pancreatic epithelium. Since N-cadherin …
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Investigating the impact of ageing on the tumour microenvironment and its crosstalk with malignant cells in pancreatic cancer
… grafted KPC (KrasLSL-G12D/+; Trp53LSL-R172H/+; Pdx1-Cre) organoid-derived mouse models of PDAC and compared them to young (~3-month-old) mouse models. Single- cell transcriptomics, graph network-based machine learning, flow cytometry and histological analyses showed that aged models have a more …
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Modulation of the tumor microenvironment by the CXCR4 antagonist AMD3100 in pancreatic and colorectal adenocarcinoma
… (TME) of PDAC. In the KRasG12D; p53R172H; Pdx1-Cre (KPC) genetically engineered mouse model of PDAC, T cells are excluded from tumour nests and this effect is related to the chemokine CXCL12 produced by FAP+ stromal cells. Targeting the CXCL12/CXCR4 pathway with AMD3100, a CXCR4 antagonist, …
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New Roles of the Transcription Factor NKX6.1 in Beta Cell Biology
… although the studies also demonstrate a role for Pdx1 and Mafbeta in mediating this suppression. Second, the silencing of Nkx6.1 expression in pancreatic beta cells results in a severe impairment in GSIS, suggesting that Nkx6.1 target genes are critical for robust GSIS. Third, Nkx6.1 is shown to …
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Chickens from lines artificially selected for juvenile low and high body weight differ in glucose homeostasis and pancreas physiology
… 2, GLUT2; and pancreatic duodenal homeobox 1, Pdx1) in LWS, than HWS chickens. Histological analysis of pancreas revealed that HWS chickens have larger pancreatic islets, less pancreatic islet mass, and more pancreatic inflammation than LWS chickens, all of which presumably contribute to …
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Einfluss von Glucolipotoxizität auf die Funktion der β-Zellen diabetessuszeptibler und –resistenter Mausstämme
… des β-zellspezifischen Transkriptionsfaktors PDX1 gezeigt wurde. Mit Ausnahme des Effekts einer Dephosphorylierung von FoxO1, konnten ob/ob-Mäuse diesen Signalweg aufrechterhalten und dadurch einen Verlust von β-Zellen abwenden. Die glucolipotoxischen Effekte wurden in vitro an isolierten …
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PTEN-LONG, a translational variant of the canonical tumor suppressor PTEN
… is recapitulated in a Kras G12D p53 H172R Pdx1-Cre (KPC) mouse model. We examined the efficacy of PTEN-Long treatment alone or in combination with gemcitabine in the KPC model. To our surprise PTEN-Long preformed equivalently to gemcitabine as a monotherapy, and the animals had a …
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Investigation of molecular and cellular events associated with beta cell function and elucidation of extracellular RNAs as potential biomarker for diabetes
… specimens from Type 2 diabetes and controls. Pdx1, Npy, Egr1, Pld1, Chgb, Ins1, Ins2, and betaactin from MIN6(L), MIN6(H), and MIN6 B1 cells and their CM suggests that beta cells transcribe and release these mRNAs into their culture environment.This study was subsequently translated to …
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Modelling human liver bud development in vitro
… pancreato-biliary progenitors (KRT19+CDX2+PDX1+HNF1B+) and extra-hepatic cholangiocytes (ACE2+MUC13+). By performing extensive snRNAseq analyses across HepBud formation, I was able to validate that hepatic specification in HepBuds follows a natural developmental path forming hepatoblast …
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The Role of Sox17 in Normal and Pathological Beta Cell
… Sox17 two-fold in a MODY4 mouse model (Pdx1-tTA hemizygote mice). Increased expression of Sox17 in the β-cells of MODY4 animals was sufficient to transiently normalize basal blood glucose and insulin levels as well as restore islet cell organization architecture; however, Sox17 …
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Structure-function analysis of the essential islet regulatory factor Nkx2.2
… An Nkx2.2-repressor fusion protein derivative (Pdx1:Nkx2.2-EnR) expressed in the Nkx2.2-/- background can fully rescue the alpha cell population, but can only specify a few immature beta cells, suggesting that Nkx2.2 must contain both repressor and activator functions to properly guide beta cell …
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Role of PARK2Gene in Maintenance of Pancreatic Beta Cell Functions
… insulin secretion. The mRNA levels of the genes Pdx1, Mafa, Mafb, Nkx2-2, Nkx6-1, Pax4, and Irs1 that encode the pancreatic transcription factors were significantly decreased in the Park2-depleted INS-1 β-cells. In addition, the INS-1 β-cells lacking parkin exhibited decreased intracellular ATP …
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