Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Pdk1"”.
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Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products
… sequence for the 5.6 kb xylGFJQK region of the pDK1 TOL meta operon was determined. DNA sequence analysis revealed the presence of five open reading frames corresponding to xylG (1458 bp), xylF (846 bp), xylJ (783 bp), xylQ (936 bp) and xylK (1047 bp), encoding predicted protein products of …
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DNA Damage-dependent Regulation and Function of akt-1 in Caenorhabditis elegans
… 3-phosphoinositide-dependent protein kinase 1 (PDK1) homologue PDK-1. This pathway ultimately controls the action of Akt homologues on the forkhead transcription factor DAF-16. The C. elegans Akt orthologue akt-1 also negatively regulates the DNA damage-dependent apoptosis of worm germ cells by …
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Dichloroacetate, a inhibitor of pyruvate dehydrogenase kinase-1, attenuates hypoxia-induced resistance to 5-fluorouracil in gastric cancer cells
… in the AGS and MKN45 cell lines, as well as in PDK1 knockdown cell lines. The expression levels of HIF-1α and PDK-1 were both elevated in the tumor tissues of most patients who showed recurrence after adjuvant 5-FU treatment. Cellular viability tests showed that these cell lines had a lower …
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Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.
… ratio. Further, we discovered that expression of PDK1 is upregulated in INS-1 832/13 cells cultured at high concentrations of glucose, and that knock-down of PDK1 enhances insulin secretion. Both these findings prove that mitochondrial metabolism is important for insulin secretion, and points to …
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Identification of a new tumor suppressor pathway modulating rapamycin sensitivity in colorectal cancer
… cancer, loss of PPP2R2B leads to induction of PDK1-dependent Myc phosphorylation in response to rapamycin. Conversely, re-expression of PPP2R2B blocks the PDK1-Myc signaling, leading to re-sensitization to rapamycin. We also show that genetic ablation or pharmacologic inhibition of PDK1 …
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Different Effect of 5-Fluorouracil on Expression of Genes Related to Aerobic Glycolysis According to Chemoresponsiveness in Gastric Cancer Cells
… glycolysis related genes (GLUT1, PGK1 and PDK1), and the rate of glucose uptake in those cells. HIF 1α was highly expressed in SNU216, and the responsiveness of SNU216 to 5-FU was lower than it of MKN45. The expressions of glucose transporter 1(GLUT1), phospho-glycerate kinase 1(PGK1) …
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Molecular Mechanisms Underlying Innate Immune Kinase TBK1-Driven Oncogenic Transformation
… and hydrophobic motif sites independently of PDK1 and mTORC2. A population of AKT is bound to components of the exocyst complex. Upon mitogen stimulation, triggering of the innate immune response, re-exposure to glucose, or oncogene activation, TBK1 is recruited to the exocyst, where it …
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Epithelial to Mesenchymal Transition As A Predictor of Response to Polo-Like Kinase 1 Inhibition-Induced Apoptosis In Non-Small Cell Lung Carcinoma
… lines and we identified β-Catenin, SMAD4 and PDK1 to be differentially regulated between epithelial and mesenchymal NSCLC cell lines after PLK1 inhibition. We tested the role of β-Catenin, SMAD4 and PDK1 in PLK1 inhibition induced apoptosis in NSCLC.</p> <p>Here, we demonstrate that …
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High-throughput functionalization of the Toxoplasma kinome uncovers a novel regulator of invasion and egress
… Ca2+ stores. Despite homology to mammalian PDK1, SPARK lacks a lipid-binding domain, suggesting a rewiring of the pathway in parasites. HiT screening extends genome-wide approaches into complex cellular phenotypes, providing a scalable and versatile platform to dissect parasite biology.
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Spatial Dynamics and the Mechanoresponse in CD4+ T Cell Activation
… with TCR and CD28. Downstream of PI3 kinase, PDK1 is identified as being essential in both TCR and CD28 costimulatory force generation; inhibition of PDK1 fully abrogates cellular forces. Lastly, we qualitatively characterize T cell activation on micropillar arrays, where their complex …
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Transcriptional Regulation of human Plasminogen Activator Inhibitor-1 Gene Expression by Insulin-like Growth Factor-1, Insulin and Upstream Stimulatory Factor-2
… in HepG2-Zellen mit dominant-negative PDK1 oder TRB3, dass Akt/PKB hemmt, hatte keinen Einfluss, während dominant-negatives Raf-1 den IGF-1 Effekt auf HIF-1 alpha wiederum vollständig aufhob. Somit aktiviert IGF-1 die humane PAI-1- Genexpression durch die Aktivierung der PI(3)K und …
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The Effects of PTEN Deletion on Cell Size and Plasticity in the Hippocampus
… and long-term depression. Deletion of Pdk1, the positive regulator of the PI3K pathway, rescued Pten-mediated deficits in synaptic plasticity. These results suggest that Pten independently modulates functional and structural properties of hippocampal neurons and is directly involved in …
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Hormone-induced expression of the epithelial sodium channel in human airway cells
… must then be phosphorylated via TORC2 and PDK1, protein kinases activated via the P13-kinase pathway. Once activated, SGK1 appears to exert control over the surface abundance of ENaC subunits by phosphorylation, and thus inactivating, a ubiquitin ligase (Nedd4-2), that normally mediate the …
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Novel Functions of Cetuximab In Egfr-Targeted Therapy: Radiosensitization, Glycolysis Inhibition, and Cellular Redox Status Regulation
… targeting pyruvate dehydrogenase kinase 1 (PDK1), in combination with cetuximab, leads to a synergetic and synthetic lethality in cancer cells, which bypasses most currently known cetuximab resistant mechanisms. In summary, our findings provide novel mechanistic insights into …
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The role of Serum- and Glucocorticoid kinase in the hormonal control of sodium transport in pulmonary epithelia
… (TORC2) and phosphoinositidedependent Kinase 1 (PDK1), both of which are dependent upon Phosphoinositide-3-Kinase (PI3K). However, SGK1 knockout mice do not display any pulmonary abnormalities. Thus the role that SGK1 plays is not fully understood. In this thesis I have explored the role of this …
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Identification of a ciliary defect associated with pulmonary nontuberculous mycobacterial disease
… of the PI-3K pathway (PI-3K , Akt1, and PDK1) closely mimicked the ex vivo phenotype seen in PNTM patient respiratory epithelia. These data identify a novel aspect of PNTM disease with in vivo and ex vivo correlates that suggest that PNTM infection is associated with abnormal function at …
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Anti-cancer effects of aqueous extracts of Dodonaea Viscosa on Burkitt lymphoma
… leading to upregulation of its target p-PDK1. Additionally, the effector molecule p-AKT (ser 473) and p-GSK3 were altered. Lastly, using a BL xenograft mouse model, DVE was found to be significantly less toxic than the approved drug Doxorubicin, and to slow down tumour growth over time. …
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The regulation and function of Forkhead box O transcription factors FOXO1 and FOXO3 in T and iNKT lymphocytes
… but repressed by the P1(3,4,5)P<sub>3</sub>/PDK1/AKT signalling pathway. Interestingly FOXO1 is not regulated by glucose or amino acid availability in CTL, which are essential regulators of CTL growth, proliferation and effector function. <br/><br/>Using FOXO1<sup>GFPflx/GFPflx</sup> Granzyme …
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PHYTOCHEMICAL AND SYNTHETIC INVESTIGATIONAL COMPOUNDS AS MODULATORS OF CYTOMETABOLIC AND CYTOSKELETAL DYNAMICS FOR PRE-CLINICAL TARGETING OF PROSTATE CANCER
… INSIG1, FASN, PKM2, PPARα/γ, mTOR, MAF, PDK1, LDLR, and SRB1), induced ER stress by elevating key targets in ER stress signaling (CHOP, ATF4, GRP94, GRP58, GADD 34, and PGC1α), increased glucose uptake and glycolysis due to severe nutrient and energy reduction in cells, and interfered …
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