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Showing 1 to 6 of 6 for “"PcrA"”.
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Single-molecule study of the DNA helicase regulating genomic stability
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination. Using single molecule FRET technique and …
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Modeling and Simulating Biomolecular Machineries at Atomic Scale and Beyond
… and ubiquitous, namely, (1) a DNA helicase PcrA, a prototype molecular motor traveling along DNA, for which we have provided evidence for an alternating domain mobilities mechanism that controls the directional translocation; (2) a membrane channel protein Aquaporin-1 (AQP1), conducting …
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IDENTIFICATION AND ANALYSIS OF PROTEINS AND GENES RESPONSIBLE FOR MICROBIAL ANAEROBIC NITRATE-DEPENDENT IRON OXIDATION AND OVEREXPRESSION IN E. coli OF PERCHLORATE REDUCTASE
… is encoded by an operon containing four genes, pcrABCD. Enzymatically active perchlorate reductase purified by traditional methods is composed of two structural subunits, PcrA and PcrB, encoded by the pcrA and pcrB genes, respectively. The lengthy traditional protein purification process and the …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
… known. The same modification on the related PcrA helicase produced the same activity increase characteristics strengthening the possibility of widespread application of this conformational control technique. Thus our results directly answer the question of the role of different conformations …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
In the last 15 years, single molecule techniques have allowed us to observe the transient, heterogeneous, and multi‐step behavior of biomolecules often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
In the last 15 years, single molecule techniques have allowed us to observe the transient, heterogeneous, and multi‐step behavior of biomolecules often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism …