Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 149 for “"Pbmcs."”.
-
Differential effects of progestogens on HIV-1 replication and host gene expression in primary PBMCs and cervical tissue explants
… women and peripheral blood mononuclear cells (PBMCs) from women, by real time quantitative PCR, western blotting and Luminex assays, in response to physiologically relevant doses of progestogens. Infection assays were performed in the absence and presence of HIV-1using HIV-1BAL-RENILLA or …
-
Reprogramming of human PBMCs into iPSCs for the In-vitro manufacture of engineered vascular grafts based on biodegradable synthetic polymers
… derived from peripheral blood mononuclear cells (PBMCs) represent a promising source given their ease of harvest combined with their pluripotent nature. Therefore, hiPSCs were generated based on PBMCs and differentiated into smooth muscle cells (SMCs) as well as endothelial cells (ECs). These …
-
Modulation of protein expression in Mycobacterium tuberculosis-stimulated TB- IRIS patient-derived PBMCs by omega n-3 polyunsaturated fatty acids
… secretome of peripheral blood mononuclear cells (PBMCs) re-stimulated ex vivo with Mycobacterium tuberculosis (Mtb) whole cell lysate (WCL), and treated with n-3 PUFA. Optimization experiments were performed on cells obtained from eight healthy donors to assess the secretome changes induced by …
-
The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin
… lines with peripheral blood mononuclear cells (PBMCs) used as controls. After stimulation with endotoxin, secretion of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon gamma (IFN-γ) were determined by ELISA. The immunogenic properties of MSCs were …
-
Effect of omega-3 polyunsaturated fatty acids on inflammatory biomarkers in chronic obstructive pulmonary disease
… release from Peripheral blood mononuclear (PBMCs), and to investigate the effect of omega-3 PUFAs, on inflammatory biomarkers released from PBMCs. Methods: Blood samples were collected from 42 subjects; patients with COPD, 15 healthy smokers (HS), and 12 healthy groups (HNS). Selected …
-
Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro
… ET in equine peripheral blood mononuclear cells (PBMCs) in vitro, and to describe the cytokine profile which is associated with the ET. Blood was collected from 6 healthy horses and PBMCs were isolated. ET was induced by culturing cells with three concentrations of endotoxin given to induce ET, …
-
Endotoxin-induced microRNA expression in equine peripheral blood mononuclear cells
… in equine peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from healthy adult horses and cultured with LPS or medium only for 2, 4 and 8 hours. Concentrations of inflammatory cytokines were measured in supernatants by immunoassay. Illumina Next-Generation Sequencing of the miRNA …
-
Equine Protozoal Myeloencephalitis. Preliminary Investigation of Protozoan-Host interactions in the horse
… peripheral blood mononuclear cells (PBMCs), suggesting a direct influence of the protozoan organism on cells of the immune system, and b) if cerebrospinal fluid (CSF) from horses with EPM has an effect on mitogen-stimulated PBMCs, suggesting that the microenvironment of the site of …
-
A NOVEL PIPELINE FOR DRUG DISCOVERY IN NEUROPSYCHIATRIC DISORDERS USING HIGH-CONTENT SINGLE-CELL SCREENING OF SIGNALLING NETWORK RESPONSES EX VIVO
… define cell signalling network abnormalities in PBMCs from drug-naïve first-onset schizophrenia patients (n=12) relative to healthy controls (n=12) which are subsequently normalized in PBMCs from the same patients (n=10) after a six week course of clinical treatment with the atypical …
-
Distinct Patterns of Ccr5 Versus Alternative Coreceptor Dependence in Non-Natural Host Versus Natural Host Simmian Immunodeficiency Virus Infection
… in primary peripheral blood mononuclear cells (PBMCs), I determined that SIV were highly CCR5-dependent in rhesus macaque PBMCs, whereas SIV were partially CCR5-independent in sooty mangabey PBMCs, implicating alternative coreceptors in infection of the natural host primary cells. A chemokine …
-
Polyunsaturated fatty acids and immune cell functions
… compared with non-supplemented PHA-stimulated PBMCs. In contrast, TGF-ß1 levels from PHA-stimulated PBMCs supplemented with a-linolenic (ALA), eicosapentaenoic (EPA) or docosahexaenoic acid (DHA) were significantly decreased suggesting that n-6 fatty acids (LA, DGLA and AA) increase in vitro …
-
Biomarcadores sanguíneos predictivos de respuesta al tratamiento anti-VEGF en degeneración macular asociada a la edad
… las células mononucleares de sangre periférica (PBMCs) de pacientes con DMAE neovascular, antes del tratamiento con ranibizumab, para identificar biomarcadores de respuesta a este fármaco. Un modelo de clasificación compuesto por 4 ácidos ribonucleicos (RNA) y 1 micro RNA (miRNA) aislado de PBMCs …
-
Zinco, invecchiamento e sistema immunitario: effetti sull'apoptosi e sulla proliferazione dei linfociti
… in vitro on peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects of different age: a group of young subjects, a group of old subjects and a group of nonagenarians. In addition, cell cycle phases were analyzed by flow cytometry in PBMCs, obtained from the subjects of the same …
-
Antiretroviral drugs differentially modulate glucocorticoid activity via the glucocorticoid receptor in vitro
… viability in peripheral blood mononuclear cells (PBMCs). In PBMCs, (1 µM) TDF, MVC and DPV alone altered basal GILZ mRNA expression and had variable, donor-specific effects on interleukin (IL)-6, IL-8, and interferon (IFN)-γ gene expression. In PBMCs from some of the nine donors tested, these ARVs …
-
Analysis of the Immunomodulatory Properties of Mycobacterium Cell Wall Fraction on the Cytokine Production of Peripheral Blood Mononuclear Cells of Healthy Dogs
… in canine peripheral blood mononuclear cells (PBMCs), using enzyme-linked immunosorbent assays (ELISA) and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for quantifying cytokine protein and mRNA transcripts, respectively. PBMCs were isolated from eight healthy dogs and …
-
THE ROLE OF GENETIC VARIANTS OF NON-STRUCTURAL PROTEIN 1 IN DENGUE PATHOGENESIS
… sNS1 production in various cell lines and human PBMCs without affecting viral-RNA replication. Infection of AG129 mice displayed severe symptoms coupled with enhanced complement activation, tissue inflammation and higher lethality. Lipidomics profiling of the purified sNS1 from wild-type and …
-
EXPLORING MITOCHONDRIAL AND REDOX VULNERABILITY IN FRIEDREICH'S ATAXIA USING GOLD NANOCLUSTERS IN HUMAN CELLULAR MODELS
… peripheral blood mononuclear cells (PBMCs) were used as an accessible and clinically relevant system to assess nanoparticle association, viability, mitochondrial membrane potential, and stress responses under basal and experimentally induced mitochondrial stress conditions. While …
-
Expression of Th17 and related cytokines according to clinical activity of Behcet's disease
… by peripheral blood mononuclear cells (PBMCs) besides increased frequencies of IL-17 and IFN-γ producing T cells in BD patients with active uveitis. It was also reported that increased expression of IL-23 p19 mRNA in erythema nodosum-like lesions in patients with active BD. Purpose: In …
-
Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection
… thereof, peripheral blood mononuclear cells (PBMCs) and TZM-bl cervical cells were used as model systems for HIV-1 infection. These cells were stimulated with P4 and E2 at concentrations mimicking the menstrual cycle phases or with levels of MPA at the upper range of peak serum levels detected …
-
CHARACTERISATION OF A NOVEL SMALL MOLECULE INHIBITOR FOR IMMUNE ACTIVATION IN TRIPLE NEGATIVE BREAST CANCER
… treatment increased peritumour accumulation of PBMCs compared to control treatment. Furthermore, combination treatment with anti-PD-L1 inhibitor Atezolizumab increased IFN-γ production by PBMCs. Interestingly, the immunomodulatory effects of CHL-4 are not mediated by Lyn kinase, but by another …
Page 1 of 8