Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Paxillin"”.
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Paxillin-dependent control of tumor angiogenesis
… lab reported that the focal adhesion protein, paxillin, is required for directional migration of fibroblasts. Endothelial cells also express paxillin and localize it in their focal adhesions. Thus, I set out to analyze whether paxillin influences directional migration of endothelial cells. When …
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Paxillin is a Novel Regulator of Xenopus Oocyte Maturation
… Here we present the scaffolding molecule, Paxillin, as a newly recognized essential regulator of meiosis in Xenopus leavis oocytes. Reduction of Paxillin expression using RNA interference and antisense oligonucleotides completely abrogates steroid-triggered meiotic resumption. Detailed …
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The Structural and Functional Study of GIT1 Paxillin Binding Domain
… by the interaction between its C terminal paxillin-binding domain (PBD) and the LD motifs of paxillin. <br /><br />In this dissertation, we determined the solution structure of rat GIT1 PBD by nuclear magnetic resonance (NMR) spectroscopy. The PBD folds into a four-helix bundle, which is …
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Unraveling the Dynamics of Ser126 Phosphorylation in Paxillin: A Focus on Phosphatase Activity
Paxillin is a focal adhesion adaptor protein essential for cell adhesion and migration, regulated by reversible phosphorylation. In endothelial cells, phosphorylation at serine 126 (Ser126) plays a key role in processes like angiogenesis and vascular permeability. This study aimed to identify the …
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Dynamics of Alpha5 Integrin, Paxillin, and Alpha-Actinin During Formation and Disassembly of Adhesions in Migratory Cells
… characterized the role of the alpha5 integrin, paxillin, and alpha-actinin in the migration process. The alpha5 integrin as well as the actin-binding protein alpha-actinin are membrane-localized in actively protruding regions of the cell. The alpha5 integrin departs from protrusive regions, as …
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Dissecting interactions within focal adhesions: studies on vinculin
Vinculin and paxillin are proteins that localise to focal adhesions. Focal adhesions are<br/>specialised sites of cell attachment between the cytoplasmic side of the cell membrane and<br/>the extracellular matrix. They consist of receptors that link extracellular matrix ligands to<br/>the actin …
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Molecular Mechanisms Of Pressure-Stimulated Cancer Cell Signaling
… studies from our laboratory have shown that paxillin is a necessary mediator in the pathway by which pressure stimulates adhesion; however, it had not been determined whether paxillin acts simply as a scaffold, or participates in diverse signaling events. I therefore hypothesized that …
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Discoveries of Targets and Novel Agents for the Treatment of Ischemic Retinopathy and Neovascular Disease
… proved fatal to mice treated with an integrin-paxillin inhibitor, 6-B345TTQ. In a physiological flow-based assay, we inhibited circulating leukocytes from interacting with an inflamed endothelium, <em>in vitro. </em>These results suggested that the reparative/inflammatory angiogenic response …
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TIS21 shifts p53-induced response of EJ bladder carcinoma cells from senescence to apoptosis
… activity. p53 increased expression of paxillin which regulated senescent morphology localizing at the edge of cell membrane. EJ cells co-expressing p53 plus TIS21 significantly reduced the expression paxillin, which was induced by p53 expression. The effect in cells infected with p53 …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
… protein, the cellular migration protein paxillin, is described. A strategy, which is applicable to any expressible protein target, is detailed for the semisynthesis of a paxillin variant with a caged phosphorylated tyrosine at residue 31 of the 557-residue protein using native chemical …
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Structural-based Investigation of FAK and P130Cas in Focal Adhesion
… in order to address how FAK interacts with paxillin through its FAT domain associating with LD motifs of paxillin. The peptidomimetic inhibitors provide opportunity as potential therapeutic targets to regulate FAK activity in FAs. In the second part I identified the binding partner of …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… between focal adhesion proteins of interest, GFP-Paxillin and FAT-mCherry, using two-photon excitation fluorescence microscopy and the utility of it as a measure of protein conformational change. The hypothesis tested is that force-induced changes in protein conformation alter inter-protein …
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The role of integrin mediated adhesion and signaling during Xenopus retinal development
… proteins, beta1 integrin, talin, vinculin, paxillin and phosphotyrosine in retinal glial cell line XR1 and in the developing Xenopus retina. These proteins are colocalized at focal adhesions located at the termini of F-actin filaments in XR1 cells. These proteins display spatial and temporal …
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Ethanol Impairs Mechanisms of Macrophage Phagocytosis and Cytokine Production
… requires recruitment of the adhesion molecules paxillin and vinculin, forming a structure we term "phagosomal adhesion". Ethanol did not alter paxillin phosphorylation during phagocytosis via the FcgammaR-pathway but suppressed vinculin phosphorylation, suggesting a defect in phagosomal adhesion …
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UNDERSTANDING THE MOLECULAR BASIS OF CELL DEATH MEDIATED BY ONCOSIS-INDUCING CYTOTOXIC ANTIBODIES
… THROUGH DEGRADATION OF ACTIN-ASSOCIATED PAXILLIN, TALIN AND ALPHA-ACTININ. THE RESULTANT MEMBRANE INJURY WAS LIKELY EXACERBATED BY THE LOSS OF MYOSIN II ACTIVITY NECESSARY FOR MEMBRANE REPAIR. CYTOTOXICITY ALSO REQUIRED CROSSLINKING OF SURFACE GLYCOPROTEINS BY THE MABS AS MONOVALENT FAB …
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Dual Assembly Nanoparticles Achieve Sustained Silencing of Znf304: A Novel Transcription Factor For Β1 Integrin
… regulates Src/focal adhesion kinase and paxillin and prevents anoikis. Targeting ZNF304 using small interfering RNA (siRNA) reduces cell survival, anoikis and migration in vitro. A novel dual assembly nanoparticle system (DANP) was designed for in vivo delivery and sustained gene …
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Serine 910 Phosphorylation of Focal Adhesion Kinase Is Critical for Costamere Assembly
… S910A-FAK mutant decreased the interaction of paxillin and vinculin within costameres. This interaction was important in stabilizing α-actinin within the protein complex, as evident by a decrease in <sub>k</sub>FRAP for α-actinin-YFP. The S910A-FAK mutant also blocked ET-1 induced NRVM …
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Computational modeling of protein-biomolecule interactions with application to mechanotransduction and antibody maturation
… protein system - the helical bundle complex of a paxillin fragment bound to the FAT domain of focal adhesion kinase (FAK) revealed several structural mechanisms responsible. Second, we used computational methods to design individual mutations computed to improve binding affinity of an …
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Elucidating how αvβ3-integrin regulates neuropilin-1’s role in tumour angiogenesis in order to improve anti-angiogenic therapy
… of both molecules significantly impairs paxillin activation and focal adhesion turnover in endothelial cells, and thus inhibits endothelial cell migration, and tumour growth and angiogenesis, even in established tumours. These findings therefore provide important pre-clinical evidence …
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Force-induced calcium concentration change and focal adhesion translocation : effects of force amplitude and frequency
… calcium dye and by infecting cells with GFP-paxillin fusion proteins. Monitoring calcium concentration changes proved unreliable for determining mechanotransduction thresholds, while a non-graded, time dependent ([similar to] minutes) steady load threshold for mechanotransduction was …
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