Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Pathway Engineering"”.
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
… were successfully engineered via rational pathway design to synthesize TAL in vivo with a maximal yield of 1.7 g/L. The direct biosynthesis of phloroglucinol from D-glucose was achieved by the discovery of a novel type III polyketide synthase, PhlD, from Pseudomonas fluorescens. Expression …
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Combinatorial biosynthetic pathway engineering for microbial production of biofuels
… comes from the optimization of the biosynthetic pathway. We investigated and developed new combinatorial strategies for the optimization of the cellobiose utilization pathway, a pathway which is important in biofuel production. One strategy focused on optimizing enzyme combinations by creating a …
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Pathway engineering for the discovery and optimized production of phosphonic acids
… feasible manner. In this work we implement pathway engineering strategies to further lower these barriers, with a focus on the class of natural products called phosphonic acids. These compounds have a stable carbon-phosphorus bond, which allow them to mimic phosphate esters and carboxylic …
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Modular pathway engineering of microbial fatty acid metabolism for the synthesis of branched acids, alcohols, and alkanes
… More recently, increasingly complex metabolic pathways have been engineered by using novel hosts, modifying natural pathways, and establishing de novo pathways with enzymes taken from a variety of pathway contexts. Highly reduced and branched alkyl chains are potentially interesting targets for …
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Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii
… organism. This thesis explores three different engineering approaches aimed at improving the quality of both aglycosylated and glycosylated proteins, with a particular focus on monoclonal antibodies, the leading class of protein biopharmaceuticals by both sales and innovation. Firstly, we …
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Inverse design of self-assembling colloids via landscape engineering
… In comparison to other techniques that utilize pathway engineering or heuristics, diffusion mapping of self-assembly systems is shown to systematically infer the low-dimensional manifold on which self-assembly occurs, detailing the structural pathway information and providing a framework for …
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Pathway optimization and engineering for biofuel production
Optimizing metabolic pathways is paramount for effective and economical production of biofuels and specialty chemicals. One such significant pathway is the cellobiose utilization pathway, identified as a promising route for efficient biomass utilization. Here we describe the simultaneous …
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Development of a new DNA-assembly method and its application for the establishment of a red light-sensing regulation system
In der hier vorgelegten Doktorarbeit wurde eine Strategie zur schnellen, einfachen und zuverlässigen Assemblierung von DNS-Fragmenten, genannt AssemblX, entwickelt. Diese kann genutzt werden, um komplexe DNS-Konstrukte, wie beispielsweise komplette Biosynthesewege, aufzubauen. Dies dient der …