Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Patch-clamp technique"”.
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Elf magnetic field influence on ION Channels studied by Patch Clamp Technique: exposure set up and "Whole Cell" measurements on Potassium currents
… ( Neuroblastoma SK-N-BE ), using the whole-cell Patch Clamp technique. Such technique is a sophisticated tool capable to investigate the electrophysiological activity at a single cell, and even at single channel level. The total potassium ion currents through the cell membrane was measured while …
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Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study
… basolateral membrane of human tubules, using the patch clamp technique. The rabbit tubule segments comprised proximal straight tubules (PST), thick ascending limbs (TAL), distal convoluted tubules (OCT), and cortical collecting ducts (CCD). The human tubule segments could not be identified, but …
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New gating states of Cystic Fibrosis transmembrane conductance regulator discovered via studying pathogenic mutations, pharmacological reagents and ATP analogs.
… for drug designs. In my PhD study, I applied patch-clamp technique to explore the gating function of CFTR. Specifically, in my thesis, we approached a more complete CFTR gating scheme through three different ways. First, we proposed two new states need to be added to the original gating scheme …
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Electrophysiological characterization of a mouse deficient for oligophrenin1: a mouse model of X-linked mental retardation
… properties of CA3 pyramidal neurons using the patch-clamp technique has been undertaken. I have shown reduced inputs of excitatory and inhibitory neurotransmission to CA3 pyramidal neurons accompanied with reduced frequency dependent facilitation of the inhibitory neurotransmission at 33Hz. …
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Physiology and pharmacology of flatworm muscle
… fibers, using whole cell configuration of the patch-clamp technique in voltage-clamp mode. Inward voltage-activated current was recorded, under conditions that suppressed outward K+ conductance, by the replacement of internal K+ with Cs+ and enhanced with 15.0 mM Ba 2+ as a charge carrier in …
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Engineering Hv1 Channel Mutations and Targeted Drug for Treatment of Hv1 Mutation Associated with Cancer
… targeting issues. The second aim employs the patch-clamp technique to investigate the effects of mutations on Hv1 channel gating and its implications for disease progression. Finally, in the third aim, we focus on the development of targeted therapeutics, utilizing 2GBI, a known Hv1 proton …
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Calcium dynamics in dendrites and spines of spiny neurons in the somatosensory ‘barrel’ cortex of the rat
… fluorescence microscopy was combined with the patch-clamp technique to study the Ca2+ dynamics in dendrites and spines of spiny neurons of layer 4 of the somatosensory cortex in acute thalamocortical brain slices of young (P13-P15) rats. Back-propagating action potentials (bAPs) resulted in a …
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Modelling neuroimmune interactions using organotypic slice cultures
… function during BCG infection, whole-cell patch clamp technique was applied to the hippocampal slice cultures. The neuronal intrinsic properties were not significantly different between infected and non-infected slices. However, tuberculin PPD (M. tuberculosis extract) moderately and …
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Chloride Channels in Normal Human Blood Monocytes and a Macrophage-like Cell Line
… macrophage-like cell line) using the whole-cell patch clamp technique. The effects of chloride channel blockers were then examined on chloride currents and on the migration and adhesion of human blood monocytes to vascular endothelial cells.|Whole-cell Cl' currents in human blood monocytes were …
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Mechanisms of ion channel activation in primary cultured and clonal cell lines
… osteoblast-like cells was investigated using the patch-clamp technique. Hypotonic shock produced cell swelling that was accompanied by large amplitude, outwardly rectifying, currents that were active across the entire physiological range of membrane potentials (-80 to +100 mV). At strong …
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Unravelling Spiral Ganglion Neuron Electrophysiology: Heterogeneity, Gene Therapy, and In-Vitro Testing Models
… of SGNs at a single-cell level using a patch-clamp technique. Three distinct SGN classes based on their spike adaptation profiles have been identified and the interspike interval of action potentials in each class has been studied. Additionally, the changes in SGN response time or …
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An electrophysiological study of the effects of resveratrol and catechin at GABAa receptors
… in HEK-293 cells using electrophysiological techniques.<.p><p>HEK-293 cells were cultured and transfected using eDNA encoding human GABA<sub>A</sub> receptors. GABA-evoked currents were recorded from HEK cells 24-48 hours following transfection. Cells were voltage clamped in the whole cell …
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Short- and long-term neuronal plasticity in hippocampal CA1 region of rat
… identified CA1 pyramidal cells using whole-cell patch clamp technique. Extracellular recordings were obtained under low magnification optical resolution by assessing field potentials evoked in the synaptic layer. AMPA-R and NMDA-R mediated responses were assessed in parallel via early and late …
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The electrophysiological effects of Endothelin-1 in human atrial myocytes
… surgery. The nystatin-perforated whole cell patch clamp technique was used at 37ºC to record ICaL and action potentials in voltage-clamp and current-clamp mode respectively. Results: The current-voltage relationship of ICaL was bell-shaped, peaking at +10 mV with a current density of -4.8±0.4 …
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Neurosteroids: endogenous analgesics?
… diabetic neuropathy.<br/>The whole-cell patch-clamp technique was used on slices of neural tissue. Electrophysiological recordings were obtained from wild type mice between the ages of 6 and 80 days in lamina II of the spinal cord, the nucleus reticularis (nRT) of the thalamus and the …
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Der spannungsgesteuerte Chloridkanal CIC-2 und seine Bedeutung in der Pathogenese von idiopathischer generalisierter Epilepsie
… electrophysiologically using the whole-cell patch-clamp technique. hClC-2 and rClC-2 hardly differ in their activation properties. Both channels are virtually closed at positive potentials and activate slowly upon hyperpolarization. However, the human ClC-2 displays slower activation and …
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Modulation of synaptic transmission by TRP channels
… at glutamatergic terminals. I have used slice patch-clamp technique to record miniature, spontaneous and evoked currents in lamina II neurons of spinal cord dorsal horn, CSTN and hippocampus. In lamina II neurons of SDH and CSTN, capsaicin, a TRPV1 agonist, robustly increased the frequency of …
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Interactions of insecticidal spider peptide neurotoxins with insect voltage- and neurotransmitter-gated ion channels
… of these insecticidal toxins using voltage-clamp and current-clamp analysis of cockroach dorsal unpaired neurons utilising the whole-cell patch-clamp technique. The ability of these toxins to modulate the gating and kinetics of both voltage- and neurotransmitter-gated ion channels were …
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Potassium channels for high-frequency action potential generation in GABAergic interneurons of Rat Hippocampus
Using the nucleated patch configuration that allowed me to investigate somatic ion channels under ideal voltage-clamp conditions, I started to examine the functional properties of Kv channels expressed in orien-alveus (OA) interneurons in the hippocampal CA1 region, a major class of dendritic …
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The CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Channel: Anion Permeation, and Regulation by Adenylyl Cyclase and ATP Hydrolysis
… either the whole-cell or excised inside-out patch configurations of the patch clamp technique. A typical myocyte cell membrane contained a total of ~1400 CFTR channels; channel density was ~0.11 μm<sup>-2</sup>. Using whole-cell CFTR Cl<sup>-</sup> conductance as an on-line assay of cAMP …
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