Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Patch-clamp electrophysiology"”.
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Functional and ultrastructural investigation of mouse and human dendritic spines
… we combine super-resolution protein imaging and patch-clamp electrophysiology to investigate the formation of nascent connections in the adult mouse cortex, and we developed a new method to apply these techniques to human neurons. In the first project presented in Chapter 2, we used …
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Acclimatization to High-Altitude, Long-Term Hypoxia Alters BK Channel Structure and Function
… molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.</p>
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Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels
… manner. By applying luciferase-based assays, patch clamp electrophysiology, and intrinsic fluorescence studies, we have identified the β9 loop at the interface of FGF14 as critical for binding to Nav1.6 channels. Based on this information, we have identified a short sequence on FGF14 and …
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Impact of Noise and Spike Initiation Properties on the Encoding and Transmission of Neural Information
… to transmit spike-based representations. Using patch clamp electrophysiology combined with optogenetics, I showed that the axon of CA1 pyramidal neurons spikes transiently in response to sustained depolarization, in contrast to the soma and axon initial segment, which spike repetitively. These …
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Connectivity of olfactory bulb dopaminergic interneurons
… To investigate this functionally, I employed patch-clamp electrophysiology, which also has the benefit of facilitating clear classification of the two subtypes—axon-bearing and anaxonic—as they have distinct electrophysiological signatures. The two subtypes showed differences in the inputs …
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Synthesis and Evaluation of the Aristotelia Alkaloids at the α3β4 Nicotinic Acetylcholine Receptor
… a calcium-based fluorescence assay and automated patch-clamp electrophysiology. Extensive subsequent analogue synthesis and assessment has identified compounds with enhanced potency and selectivity at the α3β4 nAChR relative to ARQ. Ongoing studies aim to define ARQ’s binding site and explore …
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Descending Control of Limb Movements in Drosophila melanogaster
… intact. Lastly we use in-vivo whole cell patch clamp electrophysiology to characterize the role of individual DNs in response to specific sensory stimuli and in relationship to movement. We find that there are approximately 1100 DNs in Drosophila that are distributed across six clusters. …
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Activation of Ca<sup>2+</sup>-activated K<sup>+</sup> Channels and Cell Migration by Hepatocyte Growth Factor/Scatter Factor in Madin-Darby Canine Kidney Cells.
… volume-activated membrane K<sup>+</sup> current. Patch-clamp electrophysiology and transwell migration assays were used to study the effects of HGF/SF on membrane K<sup>+</sup> currents and cell migration in MDCK II cells. HGF/SF activated membrane K<sup>+</sup> currents that increased over 24 hr, …
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CaV3.2 Channels and BKCa-Mediated Feedback in Vascular Smooth Muscle
… to whole arteries, and which entail the use of patch clamp electrophysiology, Ca2+ imaging, pressure myography, immunohistochemistry, quantitative polymerase chain reaction (qPCR) and computational modeling. In initial work, perforated patch electrophysiology was used in concert with Ca2+ …
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Hormone Regulation of NPY Neuron Activity in the Arcuate Nucleus of the Hypothalamus
… receptors present on NPY neurons, whole-cell patch clamp electrophysiology was performed on identified NPY neurons throughout the rostral-caudal extent of the arcuate nucleus of the hypothalamus. Leptin and insulin acutely hyperpolarized NPY neurons, and these effects were dependent upon …
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Alzheimer's Disease and Amyloid Beta: Effects of amyloid beta on hippocampal and dorsal root ganglion neurons' electrophyiology and morphology
… to investigate the effect of Aβ on the electrophysiology and morphology of isolated hippocampal and dorsal root ganglion (DRG) neurons in order to test whether the effects of Aβ exposure and clearance are exclusive to central nervous system neurons. Isolation and culture of hippocampal …
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T-type Calcium Channels in Arterial Smooth Muscle: Activity, Regulation and Function
… an integrative range of techniques such as electrophysiology, pressurized myography, intravascular catheterization, polymerase chain reaction, western blotting and computational modeling. Using patch clamp electrophysiology and defined pharmacology, T-type currents were successfully …
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Energetics of substrate unfolding by ClpA
… under conditions which support single-molecule patch-clamp electrophysiology. These observations made it possible to carry out pilot studies for a novel electrophysiological unfoldase assay. ClpA may be able to actively extract protein substrates from the lipid bilayer. We are currently testing …
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Investigation of Accessory Olfactory Interneuron Physiology and Function After Experience-Dependent Plasticity
… resident-intruder interactions. Using whole-cell patch clamp electrophysiology, I found that Arc-expressing IGCs display increased intrinsic excitability for several days after a single resident-intruder interaction. Further, I found that Arc-expressing IGCs maintain this increased excitability …
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Exploring the effects of classical immune activation on circuit excitability and cell viability in the mouse brain
… TNFα and IL-6 from the brain slices. I used patch-clamp electrophysiology to assess both the intrinsic electrical characteristics as well as the synaptic strength between pyramidal neurons after immune activation. I found no changes in the basic membrane properties of pyramidal neurons after …
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Plasticity of Adrenal Chromaffin Cell Function During Inflammation and Exposure to Microbe-Associated Molecular Patterns
… inflammation using Ca2+ imaging and perforated patch clamp electrophysiology. Acute and chronic GI inflammation consistently reduced high-K+-stimulated Ca2+ transients in ACCs through an inhibition of voltage-gated Ca2+ current. In contrast, systemic inflammation significantly enhanced …
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Regulation of the FGF14:Nav Interactome by a GSK-3 Centered Pathway
… confocal imaging, mass spectrometry, and patch-clamp electrophysiology, we show that alteration of this focused GSK-3 interactome, comprised of Akt, Wee1, and CK2, disrupts Nav complex assembly, changes the phosphorylation status of the FGF14 N-terminus, alters the sub-cellular …
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Investigating Mechanisms In Nociceptors Driving Ongoing Activity and Ongoing Pain
… pain.</p> <p>A combination of whole-cell patch clamp electrophysiology, pharmacology, and high content microscopy was used to define the effects of low concentrations of the injury-related proinflammatory mediator serotonin and the major pathway by which it exerts its effects. This study …
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mRNA EXPRESSION ANALYSIS OF MIDBRAIN DOPAMINE NEURONS IN RESPONSE TO NICOTINE INTAKE DURING MATURATION
… gestational nicotine in newborn rats (PN 7-14). Patch-clamp electrophysiology was used to select and collect DA and non-DA neuron in newborn rats during the first week of maturation. Then, we use microarray technique to analyze the gene expression of these neurons in response to gestational …
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