Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Patch-Clamp Techniques"”.
-
Hv1 novel peptide inhibitor extracted from tarantula venom
… pH regulation and cellular signaling. Utilizing patch clamp techniques on genetically modified Chinese Hamster Ovary cells expressing human Hv1, the study revealed that GsAF-I significantly stabilizes the closed state of these channels, thereby delaying their opening and hastening their closure, …
-
CellMincer: Self-Supervised Denoising of Functional Imaging
… beyond what can feasibly be achieved with patch clamp techniques. However, imaging platforms like Optopatch suffer from excessive detection noise, photobleaching, and an inability to organically segment and isolate neurons of interest. These drawbacks preclude its use as a true substitute …
-
Mechanistic investigation of coordinated conformational changes in multisubunit ion channels and enzymes
… ATP-binding cassette (ABC) superfamily. Using patch clamp techniques, KATP channel activity was observed directly with single-channel resolution. The results indicate that noise from stochastic channel gating is significantly reduced compared to what would be observed for identical and …
-
FEAR EXTINCTION AND INFRALIMBIC CORTEX IMPAIRMENT AFTER MILD TRAUMATIC BRAIN INJURY
… local field potential recordings, and whole-cell patch-clamp techniques to investigate how mild TBI affects IL network activity and excitability in a mouse model of TBI. Our results demonstrate that mild lateral fluid percussion injury (LFPI) significantly impairs fear extinction memory, as …
-
Human Nav1.5 F1486 deletion associated with long-QT syndrome leads to deficiency in inactivation and reduces lidocaine sensitivity
… was functionally characterized by whole-cell patch clamp techniques. Studies revealed that the deletion of F1486 causes a combination of changes including a loss-of-function alteration reflected as a substantial reduction of peak current density and a number of gain-of-function alterations …
-
Modulation of calcium channel expression in cardiac myocytes of rats during growth hormone-induced growth or normal postnatal development
… currents of cardiac myocytes were studied using patch-clamp techniques. T-type Ca$\sp{2+}$ current density of atrial myocytes was found to be positively correlated with the plasma growth hormone levels and the growth rate of atrial myocytes in normally developing Sprague-Dawley rats, adult …
-
Action of halothane on large-conductance, calcium-activated potassium channels in rat cerebrovascular smooth muscle cells
… The present experiments utilized extra cellular patch clamp techniques to investigate whether clinically relevant concentrations of halothane directly alter the biophysical properties of these channels. Cerebrovascular smooth muscle cells (CVSMCs) were dispersed from the basilar, middle, …
-
Vascular Kv7 (KCNQ) Potassium Channels As Therapeutic Targets in Cerebral Vasospasm
… Kv currents recorded using the whole-</p><p>cell patch-clamp techniques were attributed to Kv7 channel activity based on their voltage dependence of activation, lack of inactivation, enhancement by flupirtine, retigabine (selective Kv7 channel activators), and inhibition by XE991 (a selective Kv7 …
-
Role of Transient Receptor Potential (TRP) Channels in Nociception
… fluorescence imaging and whole-cell patch-clamp experiments showed that both capsaicin-induced TRPV1 response and 4alpha-phorbol 12, 13-didecanoate (4alpha-PDD)-induced TRPV4 response were observed in a proportion of the same DRG neurons, suggesting their co-expression. Incubation of …
-
Functional properties and Ca2+-dependent feedback modulation of voltage-gated Ca2+ channels in glutamatergic nerve terminals of the mammalian auditory brainstem
… (VGCCs) untersucht. Zu diesem Zweck wurden patch-clamp-Messungen an zwei Typen von großen glutamatergen Nervendigungen im auditorischen Stammhirn der Maus den Heldschen Endbulbs und den Heldschen Calyces durchgeführt. Endbulb-Terminalien zeichnen sich durch einen hohen Eingangswiderstand und …