Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 193 for “"Patch clamp"”.
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Development of planar patch clamp with potentiometric calcium ion-selective electrode
… gating the flow of ions in and out of the cell. Patch clamp technology has been the proven standard for fundamental studies of ion channel activities. However, the technique has some basic limitations: low throughput, time consuming nature of its process, need of highly skilled personnel and …
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Silicon nanopore patch clamp device for measuring single ion channel activity
… the Nobel Prize for their invention of the patch clamp, which is based on the formation of a high resistance seal between a glass micropipette and a cell membrane. This transformative method allows low noise recording of current arising from single ion channels or from an entire cell and has …
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Die Kinetik des heteromeren 5-HT3-Rezeptors: Untersuchungen mit Patch-Clamp-Technik und Computersimulationen
Der 5-HT3-Rezeptor gewinnt zunehmend pharmakologische Bedeutung, z.B. hinsichtlich der Wirkmechanismen von Antidepressiva. Diese wirken u.a. als 5-HT3-Rezeptor-Antagonisten oder hemmen die präsynaptische Serotonin-Wiederaufnahme. Darüber hinaus werden 5-HT3-Antagonisten in der Therapie des …
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Elf magnetic field influence on ION Channels studied by Patch Clamp Technique: exposure set up and "Whole Cell" measurements on Potassium currents
… ( Neuroblastoma SK-N-BE ), using the whole-cell Patch Clamp technique. Such technique is a sophisticated tool capable to investigate the electrophysiological activity at a single cell, and even at single channel level. The total potassium ion currents through the cell membrane was measured while …
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Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study
… basolateral membrane of human tubules, using the patch clamp technique. The rabbit tubule segments comprised proximal straight tubules (PST), thick ascending limbs (TAL), distal convoluted tubules (OCT), and cortical collecting ducts (CCD). The human tubule segments could not be identified, but …
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Automated cell-targeted electrophysiology in vivo and non-invasive gamma frequency entrainment
Targeted patch clamp recording is a powerful method for characterizing visually identified cells in intact neural circuits, but it requires skill to perform. We found that a closed-loop real-time imaging strategy, which continuously compensates for cell movement while approaching the cell with a …
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Functional and ultrastructural investigation of mouse and human dendritic spines
… we combine super-resolution protein imaging and patch-clamp electrophysiology to investigate the formation of nascent connections in the adult mouse cortex, and we developed a new method to apply these techniques to human neurons. In the first project presented in Chapter 2, we used …
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Acclimatization to High-Altitude, Long-Term Hypoxia Alters BK Channel Structure and Function
… molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.</p>
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Assessment of ion-selective optical nanosensors for drug screening applications
… high-content system improves on the existing patch clamp and fluorescent dye technologies that presently dominate the ion-channel screening market. This paper introduces the nanosensor technology, reviews the current market for ion-channel assays, assesses the costs associated with the …
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Cell coupling and exocytosis measured in intact mouse pancreatic islets Control of {delta}-cell secretion
Patch-clamp and capacitance measurements were applied to α-, β- and δ-cells in intact mouse pancreatic islets. The maximum rate of β-cell exocytosis during a depolarization to 0 mV was 14 granules/s, <5% of that observed in isolated β-cells. β-cell exocytosis exhibited bell-shaped voltage …
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Investigating mechanisms of biophysical diversity between phasic and tonic motor neurons
… this question, we combined in vivo whole-cell patch-clamp physiology with biophysical modeling. First, we characterized biophysical diversity between 1s and 1b MNs. To explore molecular mechanisms underlying such diversity, single-neuron PatchSeq RNA profiling experiments were carried out to …
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Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels
… manner. By applying luciferase-based assays, patch clamp electrophysiology, and intrinsic fluorescence studies, we have identified the β9 loop at the interface of FGF14 as critical for binding to Nav1.6 channels. Based on this information, we have identified a short sequence on FGF14 and …
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Charakterisierung von KCNQ-Kaliumkanälen und ihren Beta-Untereinheiten
… verursachte. Der dritte Teil bestand aus Patch clamp Messungen an Mausmodellen für die vererbbare progrediente Ertaubung DFNA2, die durch Mutationen im Gen für KCNQ4 verursacht wird. Es wurden die basalen Kaliumströme in inneren und äußeren Haarzellen von KCNQ4-Knockout-Mäusen bzw. …
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Impact of Noise and Spike Initiation Properties on the Encoding and Transmission of Neural Information
… to transmit spike-based representations. Using patch clamp electrophysiology combined with optogenetics, I showed that the axon of CA1 pyramidal neurons spikes transiently in response to sustained depolarization, in contrast to the soma and axon initial segment, which spike repetitively. These …
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Investigating Mechanisms In Nociceptors Driving Ongoing Activity and Ongoing Pain
… pain.</p> <p>A combination of whole-cell patch clamp electrophysiology, pharmacology, and high content microscopy was used to define the effects of low concentrations of the injury-related proinflammatory mediator serotonin and the major pathway by which it exerts its effects. This study …
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Investigating cestode modulation of host neuronal excitability and cell-type-specific gene expression in an in vitro mouse model of neurocysticercosis
… used singlenucleus RNA sequencing and whole-cell patch-clamp electrophysiology to investigate potential links between inflammation and network excitability at a transcriptomic and electrophysiological level. In the first part of the thesis, I found that while LPS significantly affected cell-type …
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