Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Particle analysis"”.
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Návrh projektorové soustavy transmisního elektronového mikroskopu pro metodu Single Particle Analysis
… elektronového mikroskopu (TEM) pro metodu single particle analysis (SPA). Návrh projektorové soustavy byl vytvořen v programu Electron Optical Design (EOD) verze 4.020. Série buzení jednotlivých čoček pro zvětšení projektorové soustavy v rozsahu od 50 do 10000 byla vypočítaná pomocí přístupu …
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Helical Reconstruction in RELION
… OptimisatioN (RELION), an open-source single-particle analysis package, reduces the need for user expertise by the formulation of an empirical Bayesian framework, and has yielded some of the highest resolution density maps in recent years. Prior information about the helical assemblies can be …
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Cryo-electron microscopy studies of pluripotency factor Nanog
… cryo-electron microscopy and single particle analysis were utilised to determine a 3D structure of Nanog. By performing size exclusion chromatography and analytical ultracentrifugation, it was discovered that Nanog forms large oligomers of 860 kDa which corresponds to a complex of 24 …
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Strong Optical Field Ionization of Solids
… therewith the dynamic Stark shift. Our single-particle analysis identifies this as a potential reason for the different ionization behaviour. The dynamic Stark shift increases the bandgap with increasing laser intensities thus suppressing ionization to an extent where virtual population …
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Extracellular Vesicles to Evaluate the Effects of Mesenchymal Stromal Cells in the Premature Lung
… to identify cellular origin and single particle analysis to quantify EVs. TA-EVs primarily originated from lung epithelium and immune cells. After MSC administration, total TA-EVs increased threefold within 3–4 days, though EVs from specific cell types showed no significant change. This …
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Analytical methods to investigate occupational exposure to toxic elements and their species
… novel speciation methodologies, including single particle analysis. It furthers biological monitoring research of elements that are of a carcinogenic occupational concern, i.e. inorganic arsenic, hexavalent chromium and respirable crystalline silica (RCS). Current biological monitoring methods are …
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Quantitative analysis and modeling of microembolic phenomena
… Bulk and individual properties of microembolic particles were characterized using light microscopy, SEM and optically-based particle analysis. Particulate probability distributions were created from imaging data using Matlab. Size distribution, volume, morphology and chemical properties were …
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Understanding the chemistry of atmospheric particles using single particle mass spectrometry
This thesis explores ways in which single particle mass spectrometry can be extended, whether through hardware improvements, or through the use of advanced data processing techniques to provide new kinds of aerosol chemistry measurements. Most of this work has been carried out using the Particle …
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Pyrolysis oil combustion characteristics and exhaust emissions in a swirl-stabilized flame
… by employing Mie scattering and phase Doppler particle analysis(PDPA). Particulate emissions in pyrolysis oil have been found to consist predominately of unburned fuel residues (cenospheres), rather than soot, with higher emissions than LFO. Cenosphere emissions have been found to be greater …
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Molecular mechanisms of pioneer factor ELF2 interactions with the nucleosome
… between ELF2 and a nucleosome by Cryo-EM single particle analysis. The ELF2-nucleosome structure shows that two ELF2 DBDs bind onto the nucleosome cooperatively at superhelical location (SHL) +4. The alpha-4 helix of ELF2 DNA binding domain docks into major groove DNA at preferred binding motif …
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Chemistry of carbonaceous aerosols : studies of atmospheric processing and OH-initiated oxidation
… Area (MCMA) for both bulk compositional analysis and single particle characterization. Approximately 50% of the non-volatile component of PM2.5 was found to consist of carbonaceous material, including both soot and organics. Other major components included sulfates, soil/dust and heavy …
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The effects of fuel volatility and operating conditions on sprays from pressure-swirl fuel injectors
… its radial and axial penetration. Phase-Doppler particle analysis experiments were included to track the droplet diameter and velocity at various points throughout the spray. A computational fluid dynamics model was also used to study the physics leading to the observed changes. The results show …
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Firearms discharge residues
… in Chapter 4. Chapter 5 deals primarily with the Particle Analysis method for the detection and identification of firearms discharge residue, particular attention being directed towards the particle classification system and discharge residue from mercury fulminate and Sintox primed ammunition. …
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A numerical study of laminar to turbulent evolution and free-surface interaction of a vortex ring
… in a series of hairpin vortices. A Lagrangian particle analysis was performed to determine the ring entrainment and detrainment properties and to investigate the possibility of an axial flow being generated around the circumference of the core region prior to the onset of …
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Mechanisms of mRNA substrate-selection by the Ccr4-Not deadenylase complex
… purity for rigorous biochemical and structural analysis. Here I present the purification of recombinant Ccr4-Not. An experimental system was devised to quantify the rate and pattern of the deadenylation reaction that it catalyses in vitro. Two models of Ccr4-Not regulation were characterised in …
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Characterization, Speciation, and Source Apportionment of Particles inside and from the Exhaust of Public Transit Buses Fueled With Alternative Fuels
… (SEM/EDX) instrumentation compatibility for particle analysis is addressed. The physical and chemical characterization of fine particles is carried out inside a public transit bus fueled with biodiesel (BD) and ultra low sulfur diesel (ULSD).</p><p>The three sorts of square surface patterns …
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Structure and regulatory mechanism of human uncoupling protein 1
… electron microscopy (cryo-EM) and single particle analysis were used to determine the structure. Human UCP1 was purified from yeast mitochondria and modified with nanobody fiducials to develop a sample suitable for cryo-EM analysis. The resolved structure revealed that UCP1 has a similar …
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Intestinal Handling of Food Grade Titanium Dioxide
… (fgTiO2) is a biologically persistent microparticle with an average diameter of 130 nm. It is an additive in the human diet and an excipient in some capsules, tablets, and toothpastes with a median intake of ~2.5 mg per person per day in the UK. Its impact, if any, is not understood. In …
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Structural studies of SARS-CoV-2 spike protein and vesicular coats
… I used cryo-electron microscopy and single particle analysis to characterise a series of antiviral peptides and their interaction with the S protein in solution as well as on intact virions. In the second part, I discuss my work on vesicular coat proteins. Protein coated vesicles facilitate …
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Encoded hydrogel microparticles for high-throughput molecular diagnostics and personalized medicine
… as in clinical settings. Advancements in genetic analysis, molecular diagnostics, and patient-tailored medicine require robust detection technologies that can obtain high-density information from a range of physiological samples in a rapid and cost-effective manner. Compared to conventional …
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