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Showing 1 to 5 of 5 for “"Paralog Pairs"”.

  1. The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications

    … of synthetic lethal interactions, such as paralog pairs. In recent years, a couple of studies have conducted dual-gene knockout experiments on paralog pairs using different approaches to identify synthetic lethal interactions. In this study, we conducted a meta-analysis of CRISPR genetic …

    uthsc Repository record for The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications (opens in a new tab)

  2. Determining therapeutically actionable genetic interactions in human cancer at scale using multiplexed CRISPR screening

    … and digenic perturbation of 931 prioritised gene pairs. These pairs were identified through two large-scale bioinformatic analyses. The first leveraged mutual exclusivity and co-occurrence analysis of genetic alterations across thousands of sequenced cancer genomes (Chapter 2), while the second …

    cambridge Repository record for Determining therapeutically actionable genetic interactions in human cancer at scale using multiplexed CRISPR screening (opens in a new tab)

  3. Expanding cancer therapy options through genome-scale identification of synthetic lethal paralogs

    … identify synthetic lethal human gene pairs in lung cells. Previous studies showed that less than 3% of unrelated human gene pairs are synthetic lethal, while duplicated yeast genes showed a 25% synthetic lethal hit rate. We thus chose to identify new synthetic lethal lung cancer drug …

    washington Repository record for Expanding cancer therapy options through genome-scale identification of synthetic lethal paralogs (opens in a new tab)

  4. Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens

    … these never-essentials are highly enriched for paralogs, suggesting that functional redundancy masks the detection of a substantial number of genes. Finally, I will describe our efforts to investigate functional buffering among approximately 400 candidate paralog pairs using CRISPR/enCas12a …

    uthsc Repository record for Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens (opens in a new tab)

  5. Phenotypic and genetic variation in an Apios americana breeding collection; and characterization of the HD-Zip gene family, involved in abiotic stress responses in Glycine max

    … four previously described subfamilies. Syntenic paralog pairs were retained following polyploidy in Glycine ~13 Mya. RNA-Seq analysis identified 20 differentially expressed HD-Zip genes in the roots of soybean cv. ‘Williams 82’, at least at one of the three time points (1, 6, or 12 hr) under …

    iastate Repository record for Phenotypic and genetic variation in an Apios americana breeding collection; and characterization of the HD-Zip gene family, involved in abiotic stress responses in Glycine max (opens in a new tab)