Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Paracellular"”.
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Computational Modeling of Claudin-15 Paracellular Channels
Tight junctions (TJs) regulate paracellular permeability in epithelial tissues through claudin protein assemblies, yet the molecular basis of strand flexibility, pore architecture, and ion selectivity for cation-selective claudin-15 (Cldn-15) remains incompletely defined at atomic resolution. This …
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Towards Identification of Tight Junction Components Involved in the Intestinal Paracellular Absorption of Phosphate
… and molecular identity of the intestinal paracellular Pi absorption, despite this pathway reportedly mediating the majority of Pi transport in those consuming a Western diet. Here, we investigated the individual contribution of tight junction (TJ) proteins claudin-3 and claudin-4, as well …
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Claudins and tight junction-associated MARVEL proteins at the blood-brain barrier – contribution to paracellular barrier formation
… protein at the human BBB, neither tightens the paracellular barrier nor interconnects opposing cells through loop interactions. But a contribution to the proper TJ strand morphology, possibly through interactions with other TJ associated proteins is found. Despite lack of the typical PDZ binding …
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Cellular Calcium Movements and Excitation-Contraction Coupling in Guinea Pig Atria
… 1 minute were introduced and the changes in the paracellular calcium levels were measured with the calcium sensitive micro-electrodes. The control condition produced an average increase during the interval in paracellular calcium concentration of 23.6 + 1.33 uM. Following treatment of the tissue …
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Investigation of the absorption pathways of some poorly absorbed drugs using human intestinal (CAC0-2) cell monolayers
… is via two pathways; the transcellular and paracellular pathway. Caco-2 cells, when cultured on polycarbonate filters, formed a confluent monolayer with many properties of differentiated intestinal epithelial cells, As a model of human gastro-intestinaJ tract epithelia they were used to …
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Direct and indirect interaction mechanisms of immune cells and tight junction proteins
… even transmigrate through the epithelial paracellular space to fight pathogens in the lu-men of e.g. the gut or the lung. When migrating through the paracellular space, immune cells inevitably come into contact with the tight junctions (TJ), a tight meshwork of transmembrane proteins that …
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The development of preclinical strategies for facilitation of lead candidate selection
… The BBB represents a diffusive barrier to both paracellular and transcellular movement of many compounds in and out of the brain. The main rate- limiting barriers of the BBB include exclusive tight junctions that prevent the movement of hydrophilic molecules through intercellular gaps, and …
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Analysis of a model to describe lead transport by the small intestine
… rate of lead cation transport, and indicated a paracellular route for lead transport. Certain metal cations compete with lead for transport via the paracellular route and hence decrease its rate of transit. Other metal cations increase the tissue binding of lead by a process of coprecipitation, …
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A mathematical model of the airway epithelium: Computational studies of ion and water balance
… across all membranes, and nonselective paracellular passive diffusion of sodium, potassium, and chloride. Initial conditions and transport parameters were derived from experimental results.
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The effects of ethanol and aspalathus linearis on immortalized mouse brain endothelial cells (bEnd5)
… both transcellularly and through their paracellular junctions, by structures called tight junctions (TJs) that are composed of proteins. The aim of this study was to investigate the in vitro effects of ethanol (EtOH) and fermented rooibos (Rf) on a monolayer of bEnd5 mouse brain ECs, by …
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Characterization and Molecular Analysis of Fragilysin: The Bacteroides fragilis Toxin
… rapidly increased the permeability of the paracellular barrier of epithelial cells to ions (decrease in electrical resistance across monolayers) and to larger molecules (increase in mannitol flux across monolayers). Furthermore, there is a direct effect on the tight junction proteins. …
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Transport of Antibodies and Nanoparticles across An in vitro Blood-Brain Barrier
… respectively. By applying a previously-developed paracellular transport model for the in vitro BBB, we predicted the permeability to IgG for a range of IgG diffusion coefficient in the fiber-like glycocalyx at the surface of the cell monolayer, Dfiber, from 0.001 to 0.01of its free diffusion …
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Acetaldehyde and Epithelial Junctions: Role of Pp2A and Occludin Phosphorylation
… tight junctions, protein complexes that prevent paracellular passage of molecules, are compromised. The mechanisms of this barrier disruption are the subject of current study.</p> <p>Acetaldehyde, produced as a result of ethanol breakdown by intestinal microflora as well as intestinal epithelia, …
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MOLECULAR PHYSIOLOGY OF BLOOD-BRAIN BARRIER TIGHT JUNCTIONS
… adhesion structures that seal the paracellular space for small solutes. Tight junctions are a common feature in many epithelial and endothelial tissues and a crucial component of the BBB. The BBB tight junctions are shown to be regulate a size and charge selective barrier that …
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Hemodynamic regulation of blood-brain barrier phenotype: A cooperative effort linking adherens and tight junction complexes
… the cell-cell border, in parallel with reduced paracellular solute flux. For these studies, cultured bovine brain microvascular endothelial cells (BBMvEC’s) were exposed to laminar shear stress (0-10 dynes/cm 2, 0-24 h) in conjunction with targeted inhibition strategies to delineate the …
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Computational Algorithms for Predicting Membrane Protein Assembly From Angstrom to Micron Scale
… functions. Of the wide range of barriers, the paracellular protein interfaces of epithelial and endothelial cells called tight junctions with high molecular specificities are vital for homeostasis and to maintain proper health. While the breakdown of these barriers is associated with serious …
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Computational Algorithms For Predicting Membrane Protein Assembly From Angstrom To Micron Scale
… functions. Of the wide range of barriers, the paracellular protein interfaces of epithelial and endothelial cells called tight junctions with high molecular specificities are vital for homeostasis and to maintain proper health. While the breakdown of these barriers is associated with serious …
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Modulation of drug transport by citrus fruit juices
… juice-mediated solute transport data for the paracellular and transcellular diffusive pathways and on P-gp-mediated efflux activity across the Caco-2 cells supported the hypothesis. Bi-directional digoxin transport data across the MDR1-transfected L-MDR1 cells also produced a similar …
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The development of a novel in vitro model suitable for the prediction of bioavailability
… integrity was observed, with selectivity between paracellular and transcellular markers and hydrophilic and lipophilic compounds within an homologous series of beta-blockers.
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Lysine and Glycyl-L-Sarcosine Absorption Across Ovine Forestomach Epithelium In Vitro
… mechanism involves mediated as well as possibly paracellular transport.
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