Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Papain-like protease"”.
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The roles of papain-like protease-related proteins in viral replication and host immunity
Viral papain-like proteases (PLPs)-related proteins have been shown to be actively involved in host innate immunity manipulation and virus replication. In this dissertation, the research were focused on the elucidation of biological roles of nidoviral PLPs-related proteins in innate immunity …
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Structural and functional studies of the papain-like protease 2 from mouse hepatitis virus
… and deISGylating activities of coronavirus (CoV) papain-like protease domains (PLPs) are involved in virus immune evasion. To this end, we chose PLP2 from mouse hepatitis virus (MHV) as our target of study because MHV has historically served as a model system for the study of CoVs, and it has …
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FUNCTIONAL CHARACTERIZATION OF PAPAIN-LIKE PROTEASE p48 REQUIRED FOR REPLICATION OF HYPOVIRUS CHV-1 RESPONSIBLE FOR VIRULENCE-ATTENTUATION OF THE CHESTNUT BLIGHT FUNGUS
… parasitica</italic>, encodes two leader papain-like proteases, p29 and p48, at the N-terminus of ORF A and ORF B, respectively. Protease p29 has been shown to be dispensable for hypovirus RNA replication, whereas protease p48 is essential for hypovirus RNA replication. The p48 protein can …
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A Study of Human Coronavirus NL63: Characterization of Virus Replication, Targeting of Papain-Like Protease 2 by Antivirals and Probing Virus Mediated Innate Immune Antagonism
… and characterized the activity of the viral papain-like protease 2 (PLP2) domain. Using quantitative real time PCR and imaging techniques I showed that HCoV-NL63 undergoes productive replication in the ciliated bronchial epithelial cells. To identify potential broad spectrum inhibitors of …
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Coronavirus Proteases as Therapeutic Targets: Development of Biosensors to Detect Inhibition of Protease Activity and Separation of the Multiple Functions of Coronavirus Papain-Like Proteases
… I utilized two approaches targeting the proteases encoded within all coronaviruses. The papain-like protease and 3C-like protease of coronaviruses are responsible for cleaving viral polyproteins early during infection, and this step is required for viral replication. To quantitatively …
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Molecular insights into modulation of host innate immune response by viral proteins of RNA viruses SARS-CoV, HCV and Influenza virus
… mechanism. My experiments demonstrate that papain-like protease (PLpro) encoded by SARS-CoV is a potent interferon antagonist that functions independent of its protease activity. PLpro directly interacts with IRF-3 preventing its phosphorylation, dimerization, nuclear translocation and thus …
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Competition-based CRISPR-dCas9 transcriptional control mechanisms and application of dCas9 biosensors for high-throughput, cell-based protease inhibitor screens
… to create an in vivo selection system for protease inhibitors. By leveraging a previously-described dCas9 toolkit, I create a synthetic genetic circuit that responds to SARS-CoV-2 viral protease activity. Using this circuit as an in vivo biosensor, I integrate it with a RiPP-based molecular …
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Regulation of IRF-3-Dependent Innate Immune Signaling Pathway by the PLpro Domain of Non-Structural Protein 3 (NSP3) of Severe Acute Respiratory Syndrome (SARS) Coronavirus
… associated with high mortality rates. SARS-CoV, like all other successful viruses, encode proteins that counteract the innate immune response. A number of reports have indicated the papain-like protease (PLpro) domain of SARS-CoV Non-Structural Protein 3 (NSP3) as a powerful interferon …
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Computational studies of biomolecules to explore potential therapeutics for COVID-19 and SAMD9- associated diseases
… affinities against non-structural protein 2, papain-like protease, non-structural protein 4, proof-reading exoribonuclease, membrane protein, and nucleocapsid protein. These proteins are functionally significant in the virus life cycle, making them prospective molecular targets for …
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Exploring antiviral strategies: From innate immunity modulation to viral proteins targeting
… potent antivirals; among SARS-CoV-2 proteins the Papain Like protease (PL-pro) is an important protein involved in viral life cycle and in the innate immune evasion, hence it will be investigated as potential viral target for the development of selective antiviral drugs. Finally, we will …
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Multifunctional Coronavirus Papain-Like Proteases as Targets for Antiviral Therapeutics and Vaccines
… was to study the multifunctionality of papain-like proteases (PLPs) encoded within coronavirus genomes to facilitate the development of antiviral drugs and vaccines. The viral PLPs are critical for processing the amino-terminal end of the replicase during virus replication and are …
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Coronavirus Replicase Proteins: Multifunctional Mediators of Replication and Innate Immunity Evasion
… as a large polyprotein, which is cleaved by the papain-like (PLP) and picornavirus 3C-like (3CLpro) proteases. The nsps modify host membranes to produce double membrane vesicles (DMVs) upon which the replicase-transcriptase assembles and synthesizes viral RNA. nsp3, nsp4, and nsp6 are integral …