Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 60 for “"Papain"”.
-
Papain-papaya protein interactions : resistance of papaya protein to proteolytic digestion by papain
<p>The proteolytic actjvity of papain was investigated with respect to its action on both natural substrates (native papaya protein and bovine serum albumin) and artificial synthetic substrate (benzoyl~l-arginine ethyl ester). Following removal of 30 percent and 40 percent of the amino acid …
-
Papain: a Novel Urine Adulterant.
… based on creatinine, specific gravity and pH. Papain is a cysteine protease with intrinsic ester hydrolysis capability. The primary metabolite of the psychoactive chemical in marijuana, 11-norcarboxy-delta-9-tetrahydrocannibinol (THC-COOH), was assayed by FPIA in concentrations ranging from 25 …
-
Immunochemistry of Rabbit Gamma-Globulin and Its Papain-Produced Fragments
Made available in DSpace on 2014-12-05T19:38:18Z (GMT). No. of bitstreams: 1 6305148.pdf: 6449381 bytes, checksum: 926066f501a8d4ca85654e65c806b019 (MD5) Previous issue date: 1963
-
Investigating the Structure of the Papain-Inhibitor Complex using SPR and NMR
… that play a significant role in binding CPs. Papain, a CP from Carica papaya, complexes with ICP from Leishmania mexicana. Although the individual 3-D structures of ICP and papain have been determined, as of this work, the structure of the papain-ICP complex has only been predicted, not …
-
Multifunctional Coronavirus Papain-Like Proteases as Targets for Antiviral Therapeutics and Vaccines
… was to study the multifunctionality of papain-like proteases (PLPs) encoded within coronavirus genomes to facilitate the development of antiviral drugs and vaccines. The viral PLPs are critical for processing the amino-terminal end of the replicase during virus replication and are …
-
Long-arm thiolated polyacrylamide beads and their application in coupling experiments with papain.
Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1983 .K747. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1983.
-
Chemical modification of catalytically essential functional groups in the active site of papain
… ability to inactivate the sulfhydryl proteinase papain (EC 3.4.4.10). No inactivation of the enzyme was observed with three of the reagents, diazoacetamide, triethyloxonium tetrafluoroborate, and N-ethyl-5-phenylisoxazolium-3'-sulfonate. The remaining two reagents, …
-
The roles of papain-like protease-related proteins in viral replication and host immunity
Viral papain-like proteases (PLPs)-related proteins have been shown to be actively involved in host innate immunity manipulation and virus replication. In this dissertation, the research were focused on the elucidation of biological roles of nidoviral PLPs-related proteins in innate immunity …
-
Structural and functional studies of the papain-like protease 2 from mouse hepatitis virus
… and deISGylating activities of coronavirus (CoV) papain-like protease domains (PLPs) are involved in virus immune evasion. To this end, we chose PLP2 from mouse hepatitis virus (MHV) as our target of study because MHV has historically served as a model system for the study of CoVs, and it has …
-
Effect of phospholipase C papain on feathering defect in 11% homogenized UHT processed cream
… buffer solutions of different pHs revealed that papain treatment of cream (0.075 EU/ml) induced feathering defect near pH of 5.60 while phospholipase C treatment of cream (0.75 EU/ml) did not cause coagulation of protein and fat globules at pH 5.09. Papain-treated cream flocculated in coffee with …
-
A Multifrequency Phase and Modulation Lifetime Analysis of Solvent Relaxation and Protein Dynamics (Papain, Bovine, Serum, Albumin)
Steady-state fluorescence spectroscopy has been used to analyze solvent-fluorophor interactions. Two different studies have been carried out, one with conjugates of the polar, biological label, dansylaziridine; and the other with two non-polar molecules, triphenylene and anthracene.
-
Tissue and Cell-Type Localization and Partial Characterization of a Xylem Papain-Type Cysteine Protease From Arabidopsis
Cysteine proteases are associated with xylem tracheary element differentiation. XCP1 was recently identified as a xylem-specific cysteine protease in Arabidopsis (Zhao, et al., 2000). For this study a recombinant polyhistidine-tagged XCP1 (XCP1H6) was expressed and purified from an E. coli …
-
Inhibition of Cysteine Protease by Platinum (II) Diamine Complexes
… of platinum complexes affect cysteine protease, papain enzyme are studied. This could give new ways to optimize anticancer activity. The activity of papain enzyme was measured on UV-Visible spectroscopy. The inhibition profile of papain with different platinum (II) complexes, and with different …
-
FUNCTIONAL CHARACTERIZATION OF PAPAIN-LIKE PROTEASE p48 REQUIRED FOR REPLICATION OF HYPOVIRUS CHV-1 RESPONSIBLE FOR VIRULENCE-ATTENTUATION OF THE CHESTNUT BLIGHT FUNGUS
… parasitica</italic>, encodes two leader papain-like proteases, p29 and p48, at the N-terminus of ORF A and ORF B, respectively. Protease p29 has been shown to be dispensable for hypovirus RNA replication, whereas protease p48 is essential for hypovirus RNA replication. The p48 protein can …
-
Effect of proteolytic enzyme and fiber of papaya fruit on human digestive health
… the impact of papaya fruit on human digestion. Papain is a proteolytic enzyme generally extracted from the latex of unripe papaya. Previous research has focused on evaluating papain activity from the latex of different parts of the plant; however there are no reports about papain activity in …
-
Investigation of the Efficacy of Various Proteinase-Treated "Serpula (Treponema) Hyodysenteriae" Vaccines
… proteinase type X-15, proteinase type 2 fungal, papain proteinase, and pepsin proteinase. Sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE) was performed on all bulk samples as well as control samples. The papain, pepsin, and proteinase K bulk samples were prepared into …
-
Coronavirus Proteases as Therapeutic Targets: Development of Biosensors to Detect Inhibition of Protease Activity and Separation of the Multiple Functions of Coronavirus Papain-Like Proteases
… proteases encoded within all coronaviruses. The papain-like protease and 3C-like protease of coronaviruses are responsible for cleaving viral polyproteins early during infection, and this step is required for viral replication. To quantitatively assess the inhibition by small-molecule compounds …
-
A Study of Human Coronavirus NL63: Characterization of Virus Replication, Targeting of Papain-Like Protease 2 by Antivirals and Probing Virus Mediated Innate Immune Antagonism
… and characterized the activity of the viral papain-like protease 2 (PLP2) domain. Using quantitative real time PCR and imaging techniques I showed that HCoV-NL63 undergoes productive replication in the ciliated bronchial epithelial cells. To identify potential broad spectrum inhibitors of …
-
The Immunomodulatory Functions Of Diacylglycerol Kinase Zeta On Type 2 Immune Responses
… ablation of DGKζ was sufficient to protect from papain-induced airway inflammation. Further analysis revealed that protection from papain in the absence of DGKζ might be potentially due to an impairment in IL-33 production/release in response to papain. Collectively, this thesis highlights that …
Page 1 of 3