Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"Pancreatic islets"”.
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Cryopreservation of pancreatic islets
Pancreatic islet transplantation is a curative treatment for diabetes but access to this therapy is limited by the availability of islets. Long-term cryopreservation of islets could partially address this limitation but current cryopreservation protocols, which use the toxic cryoprotectant dimethyl …
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3D bioprinting of pancreatic islets
Hintergrund/Ziel: Um Langzeitkomplikationen bei Diabetes mellitus Typ 1 (T1D) zu verhindern, können Spenderinseln transplantiert werden, was allerdings lebenslange rigorose Immunsuppression erfordert. Durch Verkapselung der Inselzellen kann die Immunsuppression umgangen werden, aber Upscaling ist …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… splice variants were compared in human and mouse islets, mouse islet cell lines, and in rat insulinoma (INS1) cells. INS-1 insulinoma cells were treated with thapsigargin to induce ER stress, which can eventually lead to apoptosis. Thapsigarin-treated INS-1 cells exhibited an increase in the ratio …
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Mechanisms underlying lipotoxicity and glucotoxicity in pancreatic islets
… known for many years that long-term exposure of pancreatic islets to a high level of free fatty acids (FFA) or glucose negatively modulates islet hormone release, the identities of signals mediating these effects are not yet fully clarified. The overall objective of this study was to investigate …
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Somatostatin inhibits insulin release via SSTR2 in the hamster pancreatic islets
… inhibition of insulin release. Isolated hamster pancreatic islets were used in the present study to test this hypothesis. Both somatostatin (1-100 nM) and nonpeptide SSTR2 agonist L-779,976 (0.1-100 nM) inhibited insulin release in a dose-dependent manner. Nonpeptide agonists for SSTR1, 3, 4, and …
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Cell coupling and exocytosis measured in intact mouse pancreatic islets Control of {delta}-cell secretion
… applied to α-, β- and δ-cells in intact mouse pancreatic islets. The maximum rate of β-cell exocytosis during a depolarization to 0 mV was 14 granules/s, <5% of that observed in isolated β-cells. β-cell exocytosis exhibited bell-shaped voltage dependence and peaked at +20 mV. At physiological …
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Therapeutic Gene Delivery to Human Pancreatic Islets for Treatment of Diabetes and the Effect of TFO on Liver Fibrosis Induced by Bile Duct Ligation
… transduction of Adv‑hVEGF‑hIL‑1Ra into human islets. The mRNA expression of hVEGF and hIL‑1Ra was more than 100 times higher than that of the non‑transduced and bicistronic plasmid transfected control islets. Transduced islets were viable as evidenced by insulin release upon glucose challenge. …
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Genetic and epigenetic influence on oxidative phosphorylation, islet function and type 2 diabetes in humans
… characterised by impaired insulin secretion from pancreatic β-cells and insulin resistance in peripheral tissues. Mitochondrial ATP production by oxidative phosphorylation (OXPHOS) is known to play a critical regulatory role in glucose stimulated insulin secretion (GSIS). The overall aim of this …
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NOVEL MECHANISMS FOR PROTEIN SYNTHESIS REGULATION DURING STRESS
… regulation of protein synthesis in the isolated pancreatic islets and ¿¿-cells. To measure protein synthesis in the pancreatic islets, we have developed a novel experimental technique by combining the islet isolation technique and deuterium water labeling method. The Akita mouse, which bears a …
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The Human Pancreatic Islet Methylome and Its Role in Type 2 Diabetes
… to determine whether epigenetic dysregulation of pancreatic islets contributes to islet dysfunction in subjects with T2D. We also assessed the relationship between genetic variation and DNA methylation. We further examined the potential use of DNA methylation in blood DNA to predict future T2D. At …
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Advancing Dynamic Imaging for Microfluidic Stimulated Tissues: Biology, Techniques, and Applications
… we show that dynamic imaging of intact ex vivo pancreatic islets and three-dimensional in vitro tumor spheroids on a custom microfluidic platform provides real-time physiological and biological information with subcellular resolution. Using this microfluidic stimulated tissue platform, we …
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Energy Homeostasis During Fasting and Fed States: Foxa2 Regulates Hepatic Amino Acid Uptake
… entirely Cl-dependent. We then examined Tcf1 -/- pancreatic islets, which are defective for arginine-induced insulin secretion. Using gene expression array analysis and semi-quantitative RT-PCR analysis on pancreatic islets from Tcf1 +/+ and Tcf1 -/- mice, we found eight arginine transporters …
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NEW-ONSET HYPERGLYCEMIA AFTER SARS-COV-2 INFECTION
… and indirect effect of SARS-CoV-2 on human pancreatic islets could possibly occur. The serum of these patients showed toxicity on human pancreatic islets despite the lack of peripheral anti-islet autoimmunity, which could be reversed by the administration of anti-interleukin-1 beta (anti …
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Mitochondrial Involvement in Pancreatic Beta Cell Glucolipotoxicity
… free fatty acid (NEFA) levels can cause pancreatic β-cell failure. The molecular mechanisms of this β-cell glucolipotoxicity are yet to be established conclusively. In this thesis by exploring mitochondrial energy metabolism in INS-1E insulinoma cells and isolated pancreatic islets, a …
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Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
<p>Insulin secreting β-cells within the pancreatic islets of Langerhans are vital to maintaining glycemic control. β-cell functional mass is lost during the progression to both Type 1 and Type 2 diabetes mellitus, resulting in hyperglycemia. Therefore, a major goal of diabetes research is to …
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NESFATIN-1 AND NESFATIN-1-LIKE PEPTIDE: NUCLEOBINDIN-1/2-ENCODED INSULINOTROPIC AND ENTEROTROPIC PEPTIDES
… acid sequence found a 77 amino acid NLP. Mouse pancreatic islets and MIN6 cells express NUCB1 mRNA and protein. NUCB1 was co-localized with insulin in mouse pancreatic islets. While treatment of cells with synthetic NLP increased preproinsulin mRNA expression and secretion, a scrambled peptide …
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Mechanisms of defective insulin secretion in type 2 diabetes
Defective insulin secretion from the pancreatic B-cells is a central feature in type 2 diabetes (T2D). There is a strong hereditary component in type T2D, but the underlying pathophysiology remains largely unknown. This thesis uses a combination of gene network analysis and cell-physiological …
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