Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Pab1"”.
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The function of PAB1 in translation and in PUF3 dependent deadenylation
… involvement of the poly(A)-binding protein (PAB1) in deadenylation and translation is well known. How PAB1 inhibits deadenylation and promotes translation is not well understood. I have analyzed PAB1 variants, containing entire domain deletions and substitutions of yeast residues with human …
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Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
… decay rates. My data demonstrates that yeast PAB1 plays both positive and negative roles in controlling deadenylation, and I have identified particular regions of PAB1 involved in controlling different aspects of the mRNA degradative process. I have found that yeast PAB1 does not play a …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… between Upfl, which is required for NMD, and PAB1 occurs via the RRM1 domain of PAB1 in the yeast Saccharomyces cerevisiae. Determining the role of this interaction during NMD was performed with pulse-chase assays using a PGKlpG mRNA. These assays revealed that the interaction between Upfl and …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… by the cytoplasmic poly(A)-binding protein Pab1. These studies elucidate domain requirements and species-specific features of Pab1-dependent regulation of Pan2-Pan3. My experiments further examine how Pan2-Pan3 may be recruited by sequence-specific RNA-binding proteins. Together, these …
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Identifying novel proteins in translation complex by using analytical ultracentrifugation with fluorescent detection system
… a one-step affinity purification with Flag-PAB1 using strains carrying translation factors and specific mRNA tagged with GFP, we identified the 78S translation complex that contains all of the major components expected of the translation initiation complex: mRNA, eIF4E, eIF4G1/eIF4G2, PAB1, …
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Functional analysis of the CAF1 protein
… with certain defects in poly(A) binding protein (PAB1) that display decreased rates of translation and deadenylation. A caf1 deletion also had a more significant effect on translation than did ccr4. These and other genetic experiments suggest a role for CAF1 in translation separate from that of …
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Mechanisms of mRNA substrate-selection by the Ccr4-Not deadenylase complex
… adaptor proteins, and the effect of the protein Pab1, which binds to the poly(A) tail. These have yielded insight into the features of the proteins and RNA sequences that are critical to deadenylation. In addition, a structural study of the Ccr4-Not complex was performed using electron …
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Identification and stoichiometric analysis of the monosomal translational complex
… closed-loop structure, eIF4E, eIF4G1/eIF4G2 and PAB1. The abundance of 77S complexes decreased with translational defects and following the stresses that cause translational stoppage. By quantitating the abundance of the 77S complex in response to different stress conditions, I observed that the …