Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"PXR"”.
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Regulation of Hepatic Drug Transporters Expression in Mice During Acute Inflammation
… with inflammation. Pregnane X receptor (PXR) and nuclear factor-κB (NF-κB) are regarded as key transcriptional factors that are modulated in response to endotoxin and cytokine signaling. This work assesses the role of NF-κB and PXR in the transcriptional regulation of hepatic transporters …
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Strategies of overexpressing retinoid X receptor and pregnane x receptor for functional studies
… binding heterodimer with pregnane X rseceptor (PXR) in response to foreign xenobiotics. In addition to RXR and PXR there are other proteins involved in the RXR/PXR signaling pathway. Many proteins involved in this pathway are still unknown. This study documents the production of RXR and PXR in a …
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Studying the Specificity of hPXR Antagonists Using a Panel of Cell-based Assays
<p>The pregnane X receptor (PXR or SXR; NR1I2) is a member of the nuclear receptor superfamily. It activates the transcription of a large network of genes including cytochrome P450 (CYP) and Mdr1 which play critical roles in chemicals metabolism and transportation. Induction of CYPs contributes to …
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Regulation of P-glycoprotein at the Blood-Brain Barrier
… transcription factor, pregnane X receptor (PXR), was also demonstrated. PXR was shown for the first time to be expressed in isolated rat brain capillaries by RT-PCR, Western blot analysis and immunostaining. Six-hour exposure of isolated capillaries to the PXR ligands, PCN or dexamethasone, …
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Age-Associated Hepatic Drug Transporter Expression and Its Implications for Pediatric Pharmacotherapyflexibility affect DNA topoisomerase I function
… and MRP2 to include pregnane X receptor (PXR), constitutive androstane receptor (CAR), farnesoid X receptor (FXR), and hepatic nuclear factor 4a (HNF4alpha). Our study revealed lower gene expression of MDR1/P-gp and MRP2 in the early infant period of development. We also identified a lower …
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Vitamin E : elucidation of the mechanism of side chain degradation and gene regulatory functions
… enzymes like CYP3A4, a pregnane X receptor (PXR)-based reporter gene assay was chosen. PXR is a nuclear receptor which regulates the transcription of genes, e.g., CYP3A4, by binding to specific DNA response elements. And indeed, as shown here, vitamin E is able to influence the expression of …
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Vom Antioxidanz zum Genregulator : transkriptionelle Regulation von Phase I- und Phase II-Enzymen durch Vitamin E und antioxidative sekundäre Pflanzeninhaltsstoffe
… zu untersuchen. Dazu wurde die Aktivierung des PXR (pregnane X receptor) und des Nrf2 (NF-E2-related factor-2) als zentrale Transkriptionsfaktoren der Phase I- bzw. Phase II-Enzyme getestet. Der Einfluss von verschiedenen Vitamin E-Formen und antioxidativen Pflanzeninhaltsstoffen in Form von …
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Chemical mixtures and interactions with detoxification mechanisms and biomarker responses in fish
… between the AhR and the pregnane-X-receptor (PXR) was also suggested in livers of the PCB-resistant killifish population from the New Bedford Harbor, Massachusetts, USA. These fish have reduced AhR-CYP1A signaling but respond to exposure to PCBs in the laboratory by increased PXR, CYP3A and …
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Safety Assessment and Chemical Fingerprint Analysis of Medicinal Plants Commonly Consumed As Herbal Teas; Rooibos (<em>Aspalathus Linearis</em>) and Moringa (<em>Moringa Oleifera</em>)
… the phytochemicals through interaction with CYP, PXR, and P gp was conducted. The results indicate that a high intake of nutraceuticals containing rooibos extracts may pose a risk of herb-drug interactions when consumed with clinical drugs that are substrates of CYP enzymes. In chapter three, a …
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Ritonavir - A Novel Multidrug Resistance Modulator in Cancer Chemotherapy and Ocular Neovascular Diseases
… enzyme (CYP3A4) and nuclear hormone receptor (PXR) was induced in response to vinblastine. This overexpression was completely neutralized when cells were cotreated with ritonavir. Uptake of [3H] lopinavir and Vivid™ assay further confirmed the functional activity of transcribed genes upon …
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Effect of obesity on hepatic drug metabolism
… transduction pathways which regulate CAR and PXR transcription factors. These results establish that obesity mediated changes are biochemically dependent and not weight dependent. In Chapter 5, we developed a proof of concept that would help generate biochemically obese hepatocytes. In absence …
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High-throughput approaches to sourcing of human hepatocytes for cell-based therapies
… or absent adult markers such as CYP3A4, CYP2A6, PXR and BSEP, concomitant with downregulation of persistent fetal marker AFP. In the final section of this thesis, we explore the therapeutic potential of identified small molecules in vivo. Using zebrafish models from the Goessling/North lab, we …