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Showing 1 to 2 of 2 for “"PURIFICATION OF L-ASPARAGINASE"”.
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Process integration of cell disruption and fluidised bed adsorption of microbial enzymes: application to the retro-design of the purification of L-asparaginase
… practical feasibility and generic applicability of the direct integration in the same time frame of cell disruption by bead milling with the capture of intracellular products by fluidised bed adsorption has been demonstrated. Pilot-scale purification of the enzyme L-asparaginase from unclarified …
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ΜΕΛΕΤΗ ΤΗΣ L-ΑΣΠΑΡΑΓΙΝΑΣΗΣ ΣΤΟ ΠΡΩΤΟΖΩΟ T. PYRIFORMIS
L-ASPARAGINASE OF TETRAHYMENA PYRIFORMIS IS A MEMBRANE BOUND ENZYME. MOST OF ITS ACTIVITY ( 80%) IS ASSOCIATED WITH MICROSOMES AND THE REMAINING WITH THE PELLICLES. INTACT PROTOZOA CAN HYDROLYZE L- ASPARAGINE AT A RATIO OF 60 NMOLE/10 6 CELLS/HR WHILE THE INFLUX OF THIS AMINOACID IS ONLY 8 NMOLE/10 …