Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"PTEN loss"”.
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PTEN loss induces epithelial-mesenchymal transition in human colon cancer cells
… lines HCT116 and SW480 were transfected with PTEN siRNA or non-targeting control. Invasiveness was measured using a modified Boyden chamber assay and migration was assessed using a scratch assay. Results. PTEN knockdown increases the invasion and migration of CRC cells and the addition of …
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Microenvironment-Induced Pten Loss By Exosomal Microrna Primes Brain Metastasis Outgrowth
… primary tumor cells with normal expression of PTEN, an important tumor suppressor, lose PTEN expression after dissemination to the brain, but not to other organs. PTEN level in PTEN-loss brain metastatic tumor cells is restored after leaving brain microenvironment. This brain …
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The Effects of PTEN Deletion on Cell Size and Plasticity in the Hippocampus
… tumor suppressor phosphatase and tensin homolog (PTEN) is the central negative regulator of the phosphatidylinositol 3-kinase (PI3K) -signaling pathway, which mediates diverse processes in various tissues. In the nervous system, the PI3K pathway modulates proliferation, migration, cellular size …
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Diagnostic and Prognostic Biomarkers for Early Prostate Cancer
… this issue by focusing on DNA methylation, and PTEN and ERG protein markers in a large biomarker study. During this study we overcame several technical challenges associated with a typical biomarker discovery/validation approach such as cohort development and sample collection, and recovery of …
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Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems
… (e.g. EGFR amplification/activation, PTEN loss, p53 loss, Ink4a/Arf loss). How these genetic changes may modulate responses to radiation and chemotherapy is not well understood. To elucidate this relationship, genetically defined mouse models have been used for both in vitro and in …
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Targeting the DNA damage response in hormone-driven cancers
… in BC and PCa. Furthermore, in Chapter 4, PTEN loss was assessed in BC and PCa as a sensitiser to DNA damage and DDR inhibitors. Nuclear PTEN has been implicated in maintenance of genome stability, and this was tested in PTEN knock-out cell line models. The results show that PTEN loss …
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Development of inducible transgenic mouse models for melanoma
… of melanoma aetiology mediated by N-Ras and PTEN genes. To achieve this, an inducible gene switch approach was employed exploiting the Cre/loxP recombinase system. This approach has the advantage of avoiding embryonic lethality and helps to minimise disease(s) in other tissue(s) that may …
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Targeting PI3K Isoforms to Improve Effectiveness of T Cells Mediated Immunotherapy
… synergy between a PI3KB inhibitor with ICB in PTEN loss melanoma, but the patient population is limited. To expand the therapeutic window in PTEN-present tumors, additional strategies are needed. Literature research identified a knowledge gap regarding the roles of PI3K isoforms in tumor cells …
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Modulation of Pi3K-Akt Pathway In Colorectal Cancer
… in 20-40% colorectal cancers (CRC) through PTEN loss (PTEN<sup>loss</sup>) and <em>PIK3CA</em> mutations (<em>PIK3CA</em><sup>mut</sup>). Allosteric AKT inhibition with MK2206 monotherapy has been ineffective in CRC patients. We investigated the impact of MK2206 on pharmacodynamic (PD) …
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The PI3-Kinase/TSC Pathway: A Role in Neural and Renal Development and Pathology
PTEN is a tumor suppressor gene and its protein product negatively regulates the PI3K/AKT pathway through counteracting the kinase function of PI3-Kinase. Loss of function of PTEN results in overactivation of AKT and in turn activates multiple AKT downstream pathways. One AKT substrate is the …
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Genetic dissection of EGFRvIII brain and spinal mouse gliomas through whole-exome sequencing and in vivo piggyBac mutagenesis forward genetic screening
… known glioma drivers (including Cdkn2a, Pten and Nf1) and novel putative partners, including genes that regulate neuronal differentiation such as Sox6 and Tcf12, and a novel regulator of the Ras pathway Spred1. RNA-sequencing confirmed the presence of fusion transcripts (transposon …
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The role of phosphoglycerate dehydrogenase in cell proliferation and tumor progression
… in a model of melanoma driven by mutant Braf and Pten loss, tumor growth is accelerated. Mice with mutant Braf normally develop growth arrested melanocytic nevi, and when PHGDH is overexpressed in this context an increased in nevi is observed and malignant melanoma arises with incomplete …
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Small Molecule Regulator of ENTPD5, and ER Enzyme in the PTEN/AKT Pathway
… proliferation and survival. Tumor supressor gene PTEN encodes a lipid phosphatase that antagonizes PI3K function and consequently inhibits downstream signaling through Akt. Dysregulation of this pathway has been found in a variety of human cancer, mainly by loss of function of PTEN, or …
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1á,25(OH)2-VITAMIN D3 IN PROSTATE: INTERSECTION WITH AKT/PTEN AXIS AND ROLE IN SENESCENCE
… in prostate cancer usually occurs due to the loss of tumor suppressor PTEN, we evaluated the effect of Pten loss on 1á,25(OH)<sub>2</sub>D<sub>3</sub>)-mediated antiproliferative effects utilizing mouse prostatic epithelial cells (MPEC) with acute in vitro Pten knockdown or knockout. We found …
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PTEN regulates the BMI1-mediated self-renewal of long-term hematopoietic stem cells in developmental and stress conditions
… observed in our laboratory that the deletion of Pten in the LT-HSCs of VE-cadherin-cre+;Ptenfl/fl mice does not lead to the loss of HSC self-renewal, raising the possibility that PTEN-mediated self-renewal regulation may be more complicated than previously reported. To understand the molecular …
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Deciphering the Functions of Ets2, Pten and p53 in Stromal Fibroblasts in Multiple Breast Cancer Models
… Ets2 as well as the tumor suppressor genes Pten and p53 in tumorigenesis using conditional knockout mouse models. Importantly, the functions of these genes are assessed in multiple breast cancer models to account for the heterogeneity observed in breast cancer. Additionally, gene expression …
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Investigating the function of PINK1 in cancer development and pathogenesis
PTEN‐induced kinase 1 (PINK1) was identified initially in cancer cells as a gene up‐regulated by overexpression of the central tumour suppressor, PTEN. Loss‐of‐function mutations in PINK1 were discovered subsequently to cause autosomal recessive Parkinsonʹs disease (ARPD). Despite much research …
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Nuclear PTEN Regulates Thymidylate Biosynthesis and Cellular Sensitivity to Antifolate Treatment
… significant promise for cancer therapy. The PTEN tumor suppressor governs a variety of biological processes, including metabolism, by acting on distinct molecular targets in different subcellular compartments. In the cytoplasm, PTEN regulates a plethora of metabolic processes through …