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Showing 1 to 4 of 4 for “"PSPG Box"”.
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Cloning and Sequencing of Glucosyltransferase (GT) Genes from Grapefruit (<em>Citrus paradisi</em>) Seedlings, Searching for 7-O-GT.
… the plant secondary product glucosyltransferase (PSPG) box and used to amplify 5’GT clones. Clone-specific primers were designed from 5’clones to amplify 3’ GT clones. We obtained 5 5’ clones and 1 3’ clone as candidate GTs. We used 3 of these clones to predict 2 compiled GT sequences. Structural …
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cDNA Cloning, Expression and Characterization of a Putative Glucosyltransferase (GT) from Grapefruit (<em>Citrus paradisi</em>) Leaves.
… gene specific primers (from conserved PSPG box) and clone specific primers (from partial 5' clones) were designed, and a compiled sequence attained using SMART RACE RT-PCR. A full-length clone was obtained using primers designed from the extreme ends of the compiled sequence. The …
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Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)
<p>Flavonoids are plant secondary metabolites that play diverse roles in plants and human health. These compounds in most part exist in the glucosylated form. Grapefruit accumulates high levels of glucosylated flavonoids. Plant secondary product glucosyltransferases (GTs) catalyze the glucosylation …
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Expression and Characterization of Putative Plant Secondary Product Glucosyltransferase Clone 10 from <em>Citrus paradisi</em> and Resolution of Multiple Bands in PGT5/6.
<p>Flavonoids are a class of plant secondary metabolites that fulfill many functions <em>in planta</em> and also benefit human health. Glucosylation of flavonoids, catalyzed by glucosyltransferases (GTs), help flavonoids perform their important roles. <em>Citrus paradisi</em> (grapefruit) is known …