Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"PRC2"”.
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CROSSTALK BETWEEN PRC1 AND PRC2 IN INTESTINAL LINEAGE COMMITMENT AND CELL IDENTITY
Polycomb Repressive Complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes, from the early stages of development to adulthood. How they functionally preserve transcriptional silencing has been deeply …
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PRC2 GATEKEEPS THE BALANCE BETWEEN DIRECT AND INDIRECT NEUROGENESIS AND CONTROLS NEURONAL MIGRATION DURING HUMAN CORTICOGENESIS
… mutations in Polycomb repressive complex 2 (PRC2) components. This complex catalyzes the trimethylation of Lysine 27 on histone 3 (H3K27me3), thereby repressing transcription during embryonic development. However, our understanding of how PRC2 dysfunction affects cortical development, and how …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… development. Notably, faulty regulation of PRC2 has been associated with a broad range of cancers, suggesting that it has a critical role in maintaining cell identity. Polycomb Repressive Complex 2 (PRC2) catalyzes the posttranslational histone modification H3K27me3, a histone modification …
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The Role of Polycomb Repressive Complex 2 in Epidermal Homeostasis and Hair Growth
Polycomb repressive complex 2 (PRC2) catalyses the methylation of ‘Lys-27’ of histone H3, leading to transcriptional repression of target genes through its catalytic subunit Enhancer of zeste homolog 1/2 (EZH1/2). PRC2 functions as a critical regulator of stem cells in mouse embryonic and adult …
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CRAMP1 drives linker histone expression to enable Polycomb repression
… the function of Polycomb Repressive complex 2 (PRC2) in human cancer cell lines. Through a CRISPR/Cas9 genetic screen in a fluorescent reporter cell line responsive to PRC2 perturbation, I identified an essential requirement for the poorly characterised gene CRAMP1 in PRC2-mediated repression. …
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Multiomic Analyses Reveal Epigenomic Alterations and New Targets of Polycomb Repressive Complex 2 in Schwann Lineage Cells and Malignant Peripheral Nerve Sheath Tumors
… improvement. Commonly, MPNSTs harbor defects in PRC2 components EED or SUZ12, leading to loss of trimethylation of histone H3 on lysine 27 (H3K27me3), a repressive epigenetic mark. Consequently, PRC2 dysfunction may induce significant epigenomic and gene expression alterations. Our research aimed …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… factor, Polycomb repressive complex 2 (PRC2) engages with chromatin (Chapter 3). PRC2 is a major epigenetic machinery that maintains transcriptionally silent heterochromatin in the nucleus and plays critical roles in embryonic development and oncogenesis. It is generally thought that …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… indicated that polycomb repressive complex 2 (PRC2)-targeted genes are aberrantly activated upon replication stress in mESCs. Genome-wide chromatin immunoprecipitation of suppressive and active histone marks revealed a global loss of H3K27me3 and the maintenance of H3K4me3 at gene promoters …
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MiR-128 controls the activity of Polycomb Repressor Complexes 1 and 2 in Neural Stem Cells: Implications of its loss in gliomagenesis.
… is mediated by two protein complexes, PRC1 and PRC2. PRC activity normally plays a key role in stem cell maintenance, but it is oncogenic in glioblastoma where it is involved in cancer stem cell maintenance and radio-resistance. Here we show that miR-128 directly targets the mRNA of BMI1 and …
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Fasting alters histone methylation in paraventricular nucleus of chick through regulating of polycomb repressive complex 2
… zeste 2) in the polycomb repressive complex 2 (PRC2) is the only methyltransferase that has been linked to catalyze this methylation reaction. In addition, SUZ (suppressor of zeste) and EED (embryonic ectoderm development) are two other key proteins in PRC2 function core that help EZH2. As …
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Epigenetic Regulation Controls Intrinsic and Extrinsic Drivers of Gastrointestinal Development
… function through Polycomb Repressive Complex 2 (PRC2)-mediated chromatin bivalency. While key lineage-determining genes possess tissue-specific patterns of chromatin accessibility, PRC2 controls WNT ligand expression in mesenchymal cells without altering accessibility. Reduction of mesenchymal …
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Functional analysis of candidate flowering time genes from the model legume Medicago truncatula
… putative Medicago Polycomb Repressive Complex 2 (PRC2) subunit VERNALISATION2 (MtVRN2) was characterised previously in the Putterill laboratory, whose mutation led to early flowering. This indicated that MtVRN2 represses the transition to flowering in Medicago, opposite to VRN2 function in …
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Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… to transcription, Polycomb Repressive Complex 2 (PRC2). I also built upon previous studies of direct enzymatic crosstalk between histone ubiquitylation and H3 lysine 79 methyltransferase, Dot1L, by investigating the plasticity of ubiquitin position in stimulation of Dot1L methyltransferase …
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CHROMATIN DISRUPTION IN HUNTINGTON'S DISEASE ALTERS DEVELOPMENT AND PRECEDES NEUROPATHOLOGY
… Polycomb (PcG), concentrandoci sui complessi PRC2 e PRC1, sia in modelli in vitro sia in vivo. Le nostre analisi hanno evidenziato alterazioni nel numero e nell’area dei domini PcG, suggerendo che nella MH l’organizzazione della cromatina sia compromessa già durante le prime fasi dello …
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TOWARD STRUCTURAL INSIGHTS INTO THE RECOGNITION OF H3K27 TRIMETHYLATION BY CANONICAL PRC1
… Canonical PRC1 complexes are recruited to PRC2 target sites through the recognition of H3K27me3 by the CBX chromodomain, while variant PRC1 complexes are defined for the presence of RYBP instead of CBX and their chromatin recruitment is independent of PRC2 localization and activity. …
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Genetic and Epigenetic Characterisation of Breast Tumours
… expression of the Polycomb repressive complex 2 (PRC2)-member EZH2 and low methylation frequency in basal-like tumours, indicating alternative epigenetic silencing mechanism in these tumours. EZH2 is the key member of PRC2 that catalyses the histone modification trimethylation of lysine 27 on …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
… I identified the Polycomb repressive complex 2 (PRC2) as a Y RNA binding complex that possessed DNA replication activity (in the human cell-free system). I showed that PRC2 plays a stimulatory role in DNA replication initiation. In summary, I have established a novel NGS method for origin …
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CHD Chromatin Remodelers Promote Epigenetic Control of Development in Arabidopsis thaliana
… repression of tissue-specific genes by the PRC2. Thus, PKL plays an important role in promoting tissue identity by facilitating deposition and/or maintenance of H3K27me3.
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Discovery and Characterization of the Polycomb Repressive Complex 1 of C. Elegans
… as bait. The Polycomb repression complex 2 (PRC2) methylates histone H3 lysine 27 at target genes to modify gene expression, and this mark is recognized by PRC1, which ubiquitylates histone H2A. In Caenorhabditis elegans, a complex of the MES-2, MES-3, and MES-6 proteins is functionally …
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Role of Polycomb Group Protein Mel18 in Hematopoietic Stem Cell Maintenance
… complexes, Polycomb repressive complex 2 (PRC2) and Polycomb repressive complex 1 (PRC1). PRC2 complex deposits the mono-, di-, and tri- methylation on histone 3 lysine 27, whereas PRC1 introduces mono-ubiquitination on histone 2A lysine 119 (H2AK119ub1). PRC1 can also regulate 3D chromatin …
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