Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"PRC1"”.
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TOWARD STRUCTURAL INSIGHTS INTO THE RECOGNITION OF H3K27 TRIMETHYLATION BY CANONICAL PRC1
… in human tumors. Polycomb Repressive Complex 1 (PRC1) catalyzes the monoubiquitination of histone H2A at lysine 119 (H2AK119ub1) and are classified as canonical (PRC1.2 and PRC1.4) and variant (PRC1.1, PRC1.3 and PRC1.6), which differ in subunit composition, chromatin recruitment and catalytic …
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CROSSTALK BETWEEN PRC1 AND PRC2 IN INTESTINAL LINEAGE COMMITMENT AND CELL IDENTITY
Polycomb Repressive Complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes, from the early stages of development to adulthood. How they functionally preserve transcriptional silencing has been deeply …
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On the Interactions of Augmin with Microtubules and the Mechanics of the Cross-Linker PRC1
… with microtubules; second, the cross-linker PRC1's frictional response resisting motion between two microtubule filaments was analyzed to determine the dependence of the frictional force on the binding conditions of the cross-linker. For the augmin complexes studied sub-second interaction …
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
… 27 (H3K27); and Polycomb Repressive Complex 1 (PRC1) with the catalytic subunits RING1A/B, which catalyze the monoubiquitylation of histone H2A at lysine 119 (H2AK119) (1,2). In PRC1, the RING1A/B subunits form a core heterodimer with one of the 6 mutually exclusive PCGF (1-6) proteins, thus …
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Discovery and Characterization of the Polycomb Repressive Complex 1 of C. Elegans
… gene expression, and this mark is recognized by PRC1, which ubiquitylates histone H2A. In Caenorhabditis elegans, a complex of the MES-2, MES-3, and MES-6 proteins is functionally analogous to the PRC2 complex, but the functional analog of PRC1, and indeed whether C. elegans has such a complex, …
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Role of Polycomb Group Protein Mel18 in Hematopoietic Stem Cell Maintenance
… 2 (PRC2) and Polycomb repressive complex 1 (PRC1). PRC2 complex deposits the mono-, di-, and tri- methylation on histone 3 lysine 27, whereas PRC1 introduces mono-ubiquitination on histone 2A lysine 119 (H2AK119ub1). PRC1 can also regulate 3D chromatin structure. Bmi1 (PCGF4) and Mel18 …
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MiR-128 controls the activity of Polycomb Repressor Complexes 1 and 2 in Neural Stem Cells: Implications of its loss in gliomagenesis.
… action is mediated by two protein complexes, PRC1 and PRC2. PRC activity normally plays a key role in stem cell maintenance, but it is oncogenic in glioblastoma where it is involved in cancer stem cell maintenance and radio-resistance. Here we show that miR-128 directly targets the mRNA of …
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Examining Intracellular Phosphorylation Gradients During Cell Division
… of a microtubule crosslinking protein PRC1 to microtubule organization during cytokinesis. I find that PRC1 depletion leads to abnormal elongation of anaphase spindle, which depends on mic rotubules. I also find that the dynamics of growing microtubule plus-ends imaged by EBl-GFP is not …
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Locus-specific proteomics identifies novel repressors of Epstein-Barr virus reactivation
… two new EBV repressors: the polycomb complex PRC1 and the nucleosome remodeler CHD4. These proteins were found to independently bind the latent EBV genome and CRISPR-Cas9-mediated depletion of either of these components resulted in spontaneous lytic reactivation, confirming their role as …
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Examining Mechanisms Regulating Microtubule Assembly and Function
… MAP, Protein Regulator of Cytokinesis 1 (PRC1), forming bundles. Current models suggest that the spindle midzone can function to facilitate or restrict chromosome movement, however it is unclear how the accumulation of PRC1 on midzone microtubule bundles impact these activities. Using …
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E2F6 in axial skeletal development and gliosis
… components of Polycomb Repressive Complex 1 (PRC1) and participates in PcG-mediated repression of Hox genes. Hox genes are required for correct patterning of the mammalian skeleton. Loss of E2f6 results in posterior axial skeletal transformations. Mice deficient for both E2f6 and Bmil, a …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
… highlighting the Polycomb Repressive Complex 1 (PRC1) subtype PRC1.3. In this project, I sought to understand the role of PRC1.3 in human pluripotency and reprogramming, and determine the PRC1.3-dependent mechanisms that modulate the acquisition of naïve pluripotency. I used CRISPR-Cas9 to delete …
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Significance of Protein Interactions in Mediating AF9 Function
… multimer containing members of Polycomb group PRC1 complex, CBX8, RING1B, and BMI1. The interaction of AF9 with other PRC1 proteins is directly mediated by CBX8 and precludes AF9-DOT1L binding. Knockdown of CBX8 with shRNA leads to decreased ENaCα mRNA levels. In contrast, CBX8 overexpression …
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Ubiquitin Conjugase UBE2K Is Essential for Normal Rat Development and Spermatogenesis
… I hypothesized that UBE2K is a component of the PRC1 complex that ubiquitinates H2A to prevent transcription as a mechanism necessary for germ cells to undergo meiosis. I showed using immunostaining, that there is an inverse correlation between H2A ubiquitin staining and UBE2K expression that is …
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DEFINING THE ROLE OF THE PCGF3-CONTAINING POLYCOMB REPRESSIVE COMPLEX 1 IN SYNOVIAL SARCOMA
… deposited by Polycomb Repressive Complex 1 (PRC1). However, the precise molecular mechanisms underlying SS18-SSX transformation remain unclear. Given SS's disruption of epigenetic regulators SWI/SNF and Polycomb complexes, my study aims to uncover genetic vulnerabilities within these networks …
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H2A.Z : a molecular rheostat for gene regulation in embryonic stem cells
… the promoter region of active genes. Conversely, PRC1 -dependent H2A.Z ubiquitination prevents Brd2 occupancy at poised, bivalent genes. Loss of H2A.Z ubiquitination of by mutation of Cterminal lysines results in a Brd2 recruitment and de-repression of bivalent genes. Moreover, inhibition of Brd2 …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
… found that Nup93 interacts with members of both PRC1 and PRC2 complexes and is enriched for the most tightly regulated Polycomb binding domains. Importantly, we found that Nup93’s association with Polycomb domains is functional as Nup93 plays a role in both the spatial folding of Polycomb domains …
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The Role of Ring1 and Rnf2 in Skeletal Muscle Regeneration
… and the distinct contributions of the Prc1 complexes subunits to its regulatory functions.
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… regulators and regulatory pathways, including PRC1, accessory PcG subunits, and DNA methylation, among others; however, we know little about how they work together to coordinate gene expression programs during lineage commitment. Thus, dissecting the function of PRC2 is critical to improve our …
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CHROMATIN DISRUPTION IN HUNTINGTON'S DISEASE ALTERS DEVELOPMENT AND PRECEDES NEUROPATHOLOGY
… (PcG), concentrandoci sui complessi PRC2 e PRC1, sia in modelli in vitro sia in vivo. Le nostre analisi hanno evidenziato alterazioni nel numero e nell’area dei domini PcG, suggerendo che nella MH l’organizzazione della cromatina sia compromessa già durante le prime fasi dello sviluppo. …
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