Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"PQS"”.
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Structural and functional elucidation of four putative PQS-binding proteins in Pseudomonas aeruginosa
… gene expression: the *las*, *rhl*, and *pqs* systems. Each of the *las*, *rhl*, and *pqs* systems rely on a receptor-autoinducer relationship: these receptor-autoinducer complexes are LasR-OdDHL, RhlR-BHL, and PqsR-PQS, respectively. When the receptors (LasR, RhlR, PqsR) bind with their …
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Synthesis and biological research of the pseudomonas aeruginosa signaling compounds PQS and N-3-oxo-dodecanoyl-L-homoserine lactone
In order to obtain a better understanding of the complex host-pathogen interactions involved in chronic infections, the Pseudomonas aeruginosa signaling molecules 3-oxo-C12-homoserine lactone and Pseudomonas quinolone signal were synthesized so that the mammalian cellular responses to their …
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Unveiling Global Roles of G-Quadruplexes and G4-22 In Human Genetics
… the location of all potential G4 sequences (PQS) in the human genome, filtered them by gene regulatory location and evaluated their expression. We also searched for mutations occurring at PQS regions using well-established mutations databases. Twenty mammalian genomes were screened for PQS …
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Nutritional modeling of bacterial infections : physiology and metabolism of Pseudomonas aeruginosa during growth in cystic fibrosis sputum
… (the Pseudomonas quinolone signal, PQS), a critical regulator of virulence factor production, during growth in CF sputum. Further, I use chemical analyses of CF sputum samples to develop a defined, synthetic medium that can be used to nutritionally model in vivo conditions. Using …
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Rapid molecular signature analysis of pseudomonas aeruginosa by direct nanomolar detection of quorum sensing and biomarker molecules at a boron-doped diamond electrode
… separation of signaling molecules PYO, HHQ, and PQS using bare CE coupled with ultraviolet (UV) detection is an example. An optimized method on a bare CE is further applied to the analysis of P. aeruginosa PAO1 cell-free culture. Another solid-phase extraction (SPE) method for the signaling …
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Investigating the role of Leucine-Responsive Regulatory Protein (Lrp) in the production and regulation of specific virulence factors in Pseudomonas aeruginosa
… Previously, Lrp has been shown to bind to PQS in <em>P. aeruginosa</em>, a key player in quorum sensing, and when <em>lrp</em> is knocked out, there is a reduction in these quorum sensing virulence factors. Leucine-responsive regulatory protein (Lrp) is a known global regulator in <em>E. …
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New methods for the asymmetric synthesis of α-alkylated ketones and 1,3-amino alcohols
… as the role of Pseudomonas quinolone signal (PQS) in quorum sensing. Herein, we report the synthesis of its biological precursor, 2-heptyl-4-hydroxy-quinoline (HHQ) and possible isosteres of PQS; the C-3 Cl, Br and I analogues. N-Methylation of the iodide was also feasible and the usefulness …
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Understanding the molecular mechanism through which aspirated bile triggers chronic Pseudomonas aeruginosa infections in respiratory disease
… Loss of Pseudomonas Quinolone Signal (PQS) production in the pigmented variants underpinned the loss of redox suppression in response to bile, perhaps a consequence of the anti-oxidant/pro-oxidant activities attributed to the PQS signalling molecule. Bile is therefore capable of …
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Influence of Indole and Mixed Culture Growth on Pseudomonas Aeruginosa Biofilm Structure
… of the P. aeruginosa strains. P. aeruginosa pqs mutant strains where also studied to see if indole induced similar effects in a non-AHL mediated QS system. At lower concentrations (0.1mM), indole induced a spike in growth rates and genes geared toward virulence mechanism of P. aeruginosa. In …
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New Sensing Modalities for Bacterial and Environmental Phenomena
… species-specific Pseudomonas Quinolone Signal (PQS), and the stationary phase indicator molecule indole. SERS substrates were formed by galvanic displacement, a substrate fabrication method that can be adapted to many SERS applications. Taken together, these new sensing modalities represent a …
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Novel collective prey evasion response of Pseudomonas aeruginosa PAO1 to soil micropredator Myxococcus xanthus DK1622
… mutants of the quorum sensing operon <em>pqs</em> and gene <em>rsaL</em>, did not possess the fold. Here we describe a novel predation resistance response observed in PAO1 and we aim to frame this predation response in the context of predation resistance responses seen in prey bacteria.</p>
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Evolution and Regulation of Virulence and Antimicrobial Resistance in Pseudomonas aeruginosa
… main interconnected QS systems (las, rhl and pqs), which are in turn modulated by the Stringent Response (SR). This system allows bacteria to sense and respond to nutrient limitations and related stresses by modulating cellular transcription patterns. Notably, it has always been assumed that …
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Statistical Analysis of Biological Interactions from Homologous Proteins
… Data Bank (PDB), Protein Quaternary Server (PQS) and Protein Interfaces, Surfaces and Assemblies (PISA) server, and compared the three biological units side-by-side. The statistical analyses show that the inconsistency within these databases and between them is significant. In order to …
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Investigating the Role of the Antimicrobial Peptide TTO-53 in the Disruption of Pseudomonas aeruginosa Biofilm
… expression in genes within the LasR, PQS, and RhlI/RhlR quorum sensing pathways. To investigate the influence of TTO-53 on the efficacy of various antibiotics, we exposed cultures of PAO1 to varying concentrations of each antibiotic and compared the colony-forming units per milliliter …
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Secondary ion mass spectrometry imaging of surface-bound microbial communities
… molecule known as Pseudomonas quinolone signal (PQS) was found to be more abundant in regions closest to the antibiotic. These results suggest that PQS is regulated independent of the other alkyl quinolones, and acts as a transient, short-range signal. In an effort with relevance to microbial …
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Leadership and Adoption of Instructional Technology in a Military Medical Learning Environment: A Case Study
… a technology personnel qualification standards (PQS) check-in process for new faculty members, (5) provide sufficient infrastructure and technical support to meet the demands of an expanding technology-based curriculum, (6) an establishment of dialogue between organizations responsible for …
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The synthesis and biological evaluation of a library of autoinducer-antibiotic conjugates
Microbial resistance to antibiotics is a serious global health threat, and the discovery of new, safe and effective antibiotics is required urgently. A new class of antibiotics, namely sideophore-antibiotic conjugates, has shown promise in initial studies. Siderophores are used by bacteria for iron …