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Showing 1 to 20 of 58 for “"PP2A"”.
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Regulation of DNA replication by PP2A family phosphatases
… actively dephosphorylated in S phase. I identify PP2A-Rts1 and PP2A-Cdc55, alongside PP4, as the phosphatases responsible for Sld3 dephosphorylation and partially of Sld2. To overcome the pleiotropy of these phosphatases hindering S phase analysis, I identify the Sld3-PP2A-RTS1 interaction site …
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PP2A-B56 isoform specificity at the centromere and kinetochore
… major mitotic processes are regulated by the PP2A-B56 phosphatase complex: the Spindle Assembly Checkpoint (SAC), kinetochore-microtubule attachments and sister chromatid cohesion. We show here that these key functions of PP2A-B56, which require its localization to either the kinetochore or …
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Acetaldehyde and Epithelial Junctions: Role of Pp2A and Occludin Phosphorylation
… <p>This project explored the roles of PP2A (a protein phosphatase) in acetaldehyde-mediated tight junction disruption, wound healing, and morphological differentiation. Intestinal epithelial cell culture in monolayers on permeable membranes were used to test the effects of acetaldehyde …
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Exploring the Regulation of Mitotic PP2A-Rts1 Activity in Saccharomyces cerevisiae
… of Cdk1 on a conserved tyrosine residue. PP2ARts1 is a phosphatase that participates in dephosphorylating the conserved tyrosine residue, tyrosine-19 (Y19). PP2ARts1 dephosphorylates phosphorylated serine and threonine residues. However, in vitro experiments suggest that in conjunction …
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PRG4/CD44/PP2A Signaling Axis: A Significant Regulator of Gout Arthritis
… NLRP3 inflammasome. Protein phosphatase-2A (PP2A) may be involved in regulating inflammatory pathways in macrophages. Proteoglycan 4 (PRG4) and its receptor CD44 and its transducer PP2A signaling axis play a biologically significant role in regulating urate crystal inflammation.</p> <p><br …
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The B55α/PP2A Holoenzyme in Cell Cycle Exit, Maturation/Differentiation, and Cancer
… and the serine/threonine protein phosphatase PP2A. Protein Phosphatase 2A (PP2A), is a serine/threonine phosphatase that functions as as a collection of trimeric holoenzymes. The trimeric PP2A holoenzyme is composed of the "A" scaffolding subunit, the "C" catalytic subunit, and a "B" …
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REGULATION OF RHOGAP DLC1 BY FAK, PP2A AND MEK/ERK IN CELL DYNAMICS
… kinase (FAK) and Protein phosphatase-2A (PP2A) to activate DLC1s GAP function. EGF stimulation not only leads to the phosphorylation of DLC1 but also that of FAK to inactivate it, thus allowing PP2A-mediated dephosphorylation at a secondary site on DLC1. This signalling cascade directly …
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A flotillin-1 és vimentin fehérjék kölcsönhatásának vizsgálata a PP2A Bα fehérjével endotél sejtekben
A flotillin, vimentin, és PP2A B fehérjék kölcsönhatásának vizsgálata, endotél sejtek használatával. Ezen fehérjék tulajdonságait ismertettem. A fehérjéket termeltettük, majd pull down és Western blot kísérletekkel megvizsgáltuk. A kísérletek eredményét részletesen taglaltam, képekkel mellékelve.
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Eukaryotic chromosome segregation: New aspects of separase regulation by securin, Cdk1, PP2A and auto-cleavage
… that it interacts with protein phosphatase 2A (PP2A). However, the functions of separase's auto-cleavage and PP2A-interaction remain enigmatic. Moreover, securin was reported to also interact with PP2A but, strangely, with a different isoform of the phosphatase. Thus, the question needs …
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Role of Protein Phosphatase-2A in Regulating Monocyte Activation by Soluble and Crystalline Uric Acid in Gout
… to active IL-1β. Protein-phosphatase-2A (PP2A) is a serine/threonine phosphatase that plays an important role in cell growth and inflammation. The prodrug Fingolimod (FTY720) and its phosphorylated active metabolite (p-FTY720) activate intracellular PP2A. We hypothesized that monocyte …
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Characterization of the Protein Phosphatase 2A Regulatory Subunit PR70
Protein phosphatase 2A (PP2A) is a phosphoserine/threonine phosphatase that controls the phosphorylation of numerous proteins in eukaryotic cells. PP2A consists of a core dimer composed of a scaffolding subunit (A-subunit) and a catalytic subunit (C-subunit) that interacts with a variety of …
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The Role of Protein Phosphatases in Regulation of Drosophila S6 Kinase by Nutrient Signaling Pathways
… to genesis of disease. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase implicated in the regulation of numerous processes, including cellular growth. Elucidating the role of PP2A in nutrient signaling pathways will aid in the understanding of the biochemical processes …
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The functional and molecular characterisation of the novel DNA damage repair protein Bod1L
… a central role in DDR by controlling MDC1 and PP2A-B56 localisation to damaged DNA. Bod1L depletion leads to micronuclei formation in undamaged cells and hypersensitivity to genotoxic stress. Bod1L binds to chromatin and is phosphorylated by ATM at sites of DNA damage. In Bod1L-depleted cells, …
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Association of Protein Phosphatase 2A with S6 Kinase Is Regulated In an MTOR-Dependent Manner
… the catalytic subunit of protein phosphatase 2A (PP2A) with S6K1. PP2A is a ubiquitously expressed phosphatase that has been implicated in many intracellular signaling pathways. In this dissertation, an association between S6K1 and PP2A was investigated to elucidate the role of this phosphatase in …
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Identification and characterization of protein phosphatase 2A mutants in Arabidopsis thaliana
… My research focuses on protein phosphatase 2A (PP2A) C subunits. PP2A is one of the most abundant types of serine/threonine phosphatases in all eukaryotic cells. Compared with animals, PP2A function is not well known in plants. Early studies using protein phosphatase inhibitors are not useful to …
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Regulation of Mitotic Spindle Assembly in Caenorhabditis elegans Embryos
… assembly 1), a putative Protein Phosphatase 2A (PP2A) regulatory subunit whose silencing causes defects in spindle formation. Upon rsa-1(RNAi), spindle poles collapse onto each other and microtubule amounts are strongly reduced. My thesis work demonstrates that RSA-1 indeed functions as a PP2A …
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Protein Phosphatase 2A Function in Response to Salt Stress in Arabidopsis thaliana: Defining Pathways and Genetic Interaction Networks
<p>Protein phosphatase 2A (PP2A) is a key regulator of many biological pathways. The PP2A heterotrimer is composed of a scaffolding/regulatory A subunit, regulatory B subunit, and catalytic C subunit. In Arabidopsis thaliana, the A, B and C subunits are encoded by 3, 17, and 5 genes, respectively. …
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Role of Extracellular Signal-Regulated Kinase (ERK) in Regulation of Intestinal Epithelial Tight Junctions
… phosphorylation and translocation of PKCζ and PP2A, b) the association of PKCζ and PP2A in occludin immunocomplex in under-differentiated and differentiated Caco-2 cell monolayers, and c) by <em>in vitro</em> incubation of recombinant PKCζ and PP2A with activated ERK. Additionally, the effect …
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Identification of a new tumor suppressor pathway modulating rapamycin sensitivity in colorectal cancer
… regulatory B subunits of protein phosphatase 2A (PP2A), was selected for further functional study. Tumor suppressor PP2A complex is a major serine/threonine phosphatase that serves as a critical cellular regulator of cell growth, proliferation, and survival. However, how its change in human cancer …
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Identification and characterization of Sgo2 interactions - Insights into dynamic chromosomal localization, mechanism of cohesin protection and putative checkpoint function
… a biochemical approach, protein phosphatase 2A (PP2A) was identified as a prominent interactor of shugoshins in this thesis. Shortly thereafter, it was reported that shugoshins protect cohesin by mediating PP2A-dependent dephosphorylation. Nevertheless, it is shown here for the first time that …
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