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Showing 1 to 20 of 42 for “"PP1"”.
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Towards understanding substrate recruitment by PP1 holophosphatases
… within the human cell is protein phosphatase 1 (PP1). This enzyme functions as a holoenzyme, with a core catalytic subunit (PP1c) bound to one or two non-catalytic subunits. These non-catalytic subunits have various proposed functions including inhibiting PP1c, aiding PP1 biogenesis, providing …
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Protein-protein interaction between multi-functional protein CAD and protein phosphastase 1 (PP1)
… Interestingly, a consensus sequence for PP1 targeting proteins is located immediately adjacent to Thr-456. Peptides were synthesized corresponding to residues 444-460 of CAD that encompasses both the PP1 consensus sequence and Thr-456 as well as two mutant peptides in which Thr-456 was …
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Regulatory Factors of Cellular Senescence in Human Diploid Fibroblast : SA-p-Erk1/2, PP1/2A, PKCα
… in vitro by protein phosphatases 1 and 2A (PP1/2A). Specific activity of PP1/2A activity significantly decreased during cellular senescence, whereas their protein expression levels did not. SA-p-Erk1/2 was most likely due to the oxidation of PP1/2A, which resulted from continuous exposure of …
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Purification and characterization of a protein phosphatase (PP1-Arch) from the archaebacterium Sulfolobus solfataricus, isolation and expression of its gene
PP1-Arch was verified as a protein phosphatase by both acid molybdate extraction and thin layer electrophoresis. Soluble fraction was prepared from <i>Sulfolobus solfataricus</i>, from which PP1-Arch was purified over 1OOO-fold by DE-52 ion-exchange, hydroxyapatite, gel filtration (G- 100), and …
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The PNUTS-PP1 Complex Acts as an Intrinsic Barrier to Kaposi's Sarcoma Associated Herpesvirus Gene Expression and Replication
… of gene expression in mammalian cells. The PNUTS-PP1 complex controls elongation rates, slowing pol II after polyadenylation sites to promote termination. The Kaposi's sarcoma-associated herpesvirus (KSHV) co-opts pol II to express its genes, but little is known about its regulation of pol II …
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Protein Phosphatase 1 at the Kinetochore Regulates Chromosome Segregation
… including Aurora B. Protein Phosphatase 1 (PP1) plays a counteracting role to Aurora B to stabilize kinetochore-microtubule attachments and silence the SAC. The regulation of PP1 to modulate these functions, however, remains enigmatic. Using the biochemical tools available in the Xenopus egg …
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Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II
… Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\sp\prime$ and 3$\sp\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and …
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Role of phosphatases in the end-on conversion process
… - BubR1- recruited PP2A-B56 and KNL1-recruited PP1 - in counteracting Aurora B kinase during the end-on conversion process. I found that BubR1-recruited PP2A-B56 plays an essential role in the process, but KNL1-recruited PP1 does not. I also investigated whether the HEC1/Ndc80 N-tail is a …
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Analysis of the functions of phosphoprotein phosphatase 1 catalytic subunits during cell division
… cell is the phosphoprotein phosphatase 1 (PP1) family of phosphatases. These phosphatases are holoenzymes composed of one of three catalytic subunits -α, β, and γ - in complex with one or two regulatory subunits. There is some evidence indicating that the three PP1 catalytic subunits might …
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Purification of protein phosphatases and 14-3-3-binding proteins and their regulation by the cAMP–PKA pathway
… activated and study the isoform specificity of PP1. Most protein phosphatases of the PPP family do not exist as free catalytic subunits: instead, they interact with regulatory subunits, which specify, target and/or inhibit their activities. Protein phosphatases of the PPP family such as PP1 and …
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Mechanisms and consequences of regulating neurabin and spinophilin's interaction with the tumor suppressor protein p140CAP
… are not well understood. Spn and Nrb are PP1 targeting proteins that target PP1 to other substrates, resulting in dephosphorylation and alteration of function. We found that PP1 increases Spn association with SRCIN1, but decreases Nrb association with SRCIN1, indicating that the two …
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Regulation of the unfolded protein response by GADD34 and CReP
… of GADD34 and GADD34 bind protein phosphatase 1 (PP1) to dephosphorylate eIF2α and brings the cell back into homeostasis. CReP, similar to GADD34, binds to PP1, to dephosphorylate eIF2α. The RVxF motif, RARA sequence, and amino acids throughout the GADD34 sequence play a role in PP1 binding and …
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Spinophilin Signaling: Impacts on Body Weight, Obesity, and Beta-Cell Function
… protein scaffolding, and protein phosphatase 1 (PP1)-targeting protein that can regulate protein. Spinophilin has multiple actions. Spinophilin can bundle filamentous actin to modulate the cellular cytoskeleton. Spinophilin also mediates substrate phosphorylation by targeting and modulating PP1 …
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Structural and functional characterisation of pUL21, an α-herpesvirus phosphatase adaptor protein
… partners of pUL21: protein phosphatase 1 (PP1) and ceramide transfer protein (CERT). This thesis presents biophysical characterization of the direct interactions between pUL21 and these partners and describes the role of pUL21 as a novel viral phosphatase adaptor that recruits PP1 to …
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Analysis of cell cycle surveillance mechanisms in meiosis
… the delay is modulated by protein phosphatase 1 (PP1), whose activity allows cells to overcome the checkpoint dependent delay in a process called adaptation. In this work, experiments in the budding yeast Saccharomyces cerevisiae are described that show that premature adaptation is prevented by …
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The eIF2 phosphatase: characterization and modulation
… preferentially expressed during the ISR is PPP1R15A, a regulatory subunit of Protein Phosphatase 1 (PP1). The PP1/PPP1R15A holophosphatase dephosphorylates eIF2 and terminates the ISR once the stresses are resolved. Hence, eIF2 kinases and phosphatases work together to control levels of …
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Understanding Host-Pathogen Interactions of Rift Valley Fever Virus That Contribute to Viral Replication
… and not entry or egress. Conversely, CK1α and PP1α were identified as binding partners of the L protein using similar methods. CK1α, a kinase, and PP1α, a phosphatase, were chosen for further verification due to data demonstrating the L protein is phosphorylated on at least one serine residue, …
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INVESTIGATING THE BASES OF THE SPECIFICITY OF THE RESTRICTOR COMPLEX FOR THE TERMINATION OF NONCODING TRANSCRIPTION
… loci where PNUTS’ phosphatase binding partner PP1 is known to act. We show that among the PNUTS-bound RNAs are Restrictor-sensitive ncRNAs, thereby providing a basis for the Restrictor-PNUTS synergy recently reported. Finally, I show evidence of sequence specificity of PNUTS’ RNA binding, …
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Charakterisierung von ausgewählten Protein-Phosphatasen und MATE-Proteinen aus Arabidopsis thaliana
… die Genexpression der Protein Phosphatase-gene TOPP1, TOPP2, TOPP5, STH1 und STH2 analysiert. Alle fünf ausgewählten Gene kodieren für PP des PP1/PP2A-Typs. Es wurde untersucht, ob homologen PP-Isoformen individuelle Expressionsmuster zugewiesen werden konnten. Besonderes Augenmerk richtete sich …
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Identification of the RNA Cis-Elements that Interact with SRp30a to Regulate the Alternative Splicing of Caspase 9 Pre-mRNA
… via the action of protein phosphatase-1 (PP1). Two RNA splice variants are derived from the caspase 9 gene, pro-apoptotic caspase 9a and anti-apoptotic caspase 9b, via alternative splicing by either the inclusion or exclusion of an exon 3, 4, 5, and 6 cassette. In this study, the link …
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