Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"POLY{A}-POLYMERASE"”.
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Poly(A) Polymerase 1 (PAPS1) influences organ size and pathogen response in Arabidopsis thaliana
Polyadenylation of pre-mRNAs is critical for efficient nuclear export, stability, and translation of the mature mRNAs, and thus for gene expression. The bulk of pre-mRNAs are processed by canonical nuclear poly(A) polymerase (PAPS). Both vertebrate and higher-plant genomes encode more than one …
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Identifying Subunit Organization and Function of The Nuclear Rna Exosome Machinery
… complex is comprised of an RNA helicase and a poly(A) polymerase, as well as an RNA-binding subunit. However, it is currently unclear how the TRAMP complex is specifically assembled and moreover, if it is essential for life. The poly(A) polymerase subunit consists of a catalytic domain, as well …
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The Role of the Human Nuclear Poly(A) Binding Protein in RNA Decay
… Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human cells, a function mediated by the ubiquitous nuclear poly(A) binding protein PABPN1. We show that PABPN1 is required for the degradation of a viral nuclear …
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Architecture of eukaryotic mRNA 3' end processing machinery and insights into the mechanism of polyadenylation
… all eukaryotic messenger RNAs (mRNAs) have a polyadenosine (polyA) tail at their 3′ end that is added by a multi-protein complex known as cleavage and polyadenylation factor (CPF). CPF, along with accessory cleavage factors (CF) IA and IB, cleaves the pre-mRNA within the 3' untranslated region …
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General Transcription Factors Play Dual Roles In Initiation And Termination
… we show that TFIIB associates with poly(A) polymerase and the CF1 complex in yeast cells to form a holo-TFIIB complex. This complex was resistant to MNase digestion and brief exposure to high salt. The sedimentation coefficient of the holo-TFIIB complex was intermediate between that …
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Functional specialization of Arabidopsis poly(A) polymerases in relation to flowering time and stress
Polyadenylation is a decisive 3’ end processing step during the maturation of pre-mRNAs. The length of the poly(A) tail has an impact on mRNA stability, localization and translatability. Accordingly, many eukaryotic organisms encode several copies of canonical poly(A) polymerases (cPAPs). The …
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Cross-regulation and interaction between eukaryotic gene regulatory processes
… Another pre-mRNA processing step is cleavage and polyadenylation, which determines the 30 end of the mature mRNA. We found that 3P-Seq was able to quantify the levels of 30 end isoforms, in addition to the method's previous use for annotating mRNA 30 ends. Using 3P-Seq and a transcriptional …
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Regulation of glucosamine-6-phosphate synthase synthesis by a hierarchical acting cascade composed of two small regulatory RNAs in <i>Escherichia coli</i>.
… der bakteriellen Zellwand und der Lipopolysaccharide. Das Enzym Glukosamin-6-Phosphat Synthase katalysiert die Bildung von Glukosamin-6-Phosphat (GlcN6P) aus Fruktose-6-Phosphat und Glutamin. Dies ist der erste Schritt der Aminozuckerbiosynthese. In Escherichia coli wird GlmS zusammen …
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ΜΕΛΕΤΗ ΕΝΖΥΜΩΝ ΜΕΤΑΒΟΛΙΣΜΟΥ ΤΟΥ ΠΟΛΥ(Α) ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΔΙΕΓΕΡΜΕΝΑ ΑΠΟ ΦΥΤΟΑΙΜΟΣΥΓΚΟΛΛΗΤΙΝΗ
ΜΕΛΕΤΗΘΗΚΑΝ ΤΑ ΕΠΙΠΕΔΑ ΕΝΕΡΓΟΤΗΤΑΣ ΤΩΝ ΕΝΖΥΜΩΝ ΜΕΤΑΒΟΛΙΣΜΟΥ ΤΟΥ ΠΟΛΥ(Α) (ΠΟΛΥ(Α)-ΠΟΛΥΜΕΡΑΣΗΣ, ΠΟΛΥ(Α)-ΕΝΔΟΝΟΥΚΛΕΑΣΗΣ ΚΑΙ ΠΟΛΥ(Α)- ΕΞΩΝΟΥΚΛΕΑΣΗΣ) ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΑΝΘΡΩΠΙΝΩΝ ΠΕΡΙΦΕΡΙΚΩΝ ΛΕΜΦΟΚΥΤΤΑΡΩΝ ΦΥΣΙΟΛΟΓΙΚΩΝ ΚΑΙ ΔΙΕΓΕΡΜΕΝΩΝ ΜΕ ΤΟ ΜΙΤΟΓΟΝΟ ΦΥΤΟΑΙΜΟΣΥΓΚΟΛΛΗΤΙΝΗ (ΡΗΑ). Η ΜΕΛΕΤΗ ΕΝΤΟΠΙΣΤΗΚΕ …