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Showing 1 to 12 of 12 for “"PNPP"”.
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Protein phosphatases and protein kinases in dictyostelium
… phosphatases, 6 paranitrophenyl phosphate (pnpp) phosphatases and 2 protein kinases from <i>Dictyostelium</i> are described. .Two of the protein phosphatases are shown to have subunit structures. All of the protein phosphatase activities described were able to dephosphorylate sites on …
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Community Composting: Public-Nonprofit Partnerships and Equity in New York City Organic Waste Programs
… York City, and the public-nonprofitpartnership (PNPP) efforts that the City’s Department of Sanitation (DSNY) has employed to realize its compost education, outreach, collection, and processing efforts. These PNPPs evolved in the context of an ecosystem of nonprofit and private sector actors, as …
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Cerium oxide Nanoparticles: Their Phosphatase Activity and its Control
… activity include para-nitrophenyl phosphate (pNPP), 4-methylumbelliferyl phosphate (MUP) and adenosine triphosphate (ATP). To highlight the role of adsorbed water, we also performed experiments on pNPP with methanol as a solvent. The activity was measured by absorbance (pNPP and ATP) or …
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Preparation and characterization of cross-linked chitosan beads for phosphate adsorption in aqueous solution
… (HPO42-) species and p-nitrophenolate phosphate (PNPP) at pH 8.5 and 295 K. Several model equations were used to analyze the adsorption data, as follows: Langmuir, Sips, and Dubinin–Radushkevich (D-R) isotherms. The Sips model provided adsorption parameters for the chitosan bead systems: (i) the …
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Biophysical Insights into Lipid Oxidation Effects on Membrane Self-Organization and Enzymatic Catalysis Under Extreme Conditions
… reaction of para-nitrophenyl palmitate (pNPP) and glycerol under extreme temperature conditions. Electrospray Ionization Mass Spectrometry (ESI-MS) and spectrophotometric analyses demonstrated the successful formation of high-value products, namely para-nitrophenol (pNP) and glyceryl …
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Novel approaches to the low-cost, portable and rapid detection of bacterial pathogens in foods and food-processing environments
… (INT) to formazans. Nitrophenyl phosphate (PNPP) is present in dormant bacteria, and cleaves phosphate groups from para-nitrophenyl-phosphate salts, resulting in para-nitrophenol. Combined use of enzymatic substrates, including INT and 5-methylphenazin-5-ium methyl sulfate (PMS) for …
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Identification of a Low Molecular Weight Protein Tyrosine Phosphatase and Its Potential Physiological Substrates in Synechocystis sp. PCC 6803
… Vmax (3.2 mmole/min/mg) values for SynPTP toward pNPP are similar to those of other known bacterial low molecular weight PTPs. The protein phosphatase activity of SynPTP was inhibited by sodium orthovanadate, a known inhibitor for tyrosine phosphatases, but not by okadaic acid, an inhibitor for …
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Assessing the Phytoextraction of Organic and Inorganic Pollutants From Point Pelee National Park, Ontario and Iqaluit, Nunavut
… gerardii (one commercial and one collected from PNPP) were grown in PPNP soil contaminated with DDT concentrations of 1,059 ng/g (low) and 5,071 ng/g (high). Mean levels of DDT in plant shoots were significantly higher (100.64 ± 33.09 ng/g) in the high contaminated soil but did not statistically …
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The Role of 5' Nucleotidase in the Regulation of Morphogenesis in Dictyostelium Discoideum
… an artificial substrate p-nitrophenol phosphate (pNPP). An activity stain on non-denaturing gels with Nitro Blue Tetrazolium (NBT) and 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) as the substrate was also used. A polypeptide of approximately 90 kDa was associated with 5NU enzyme activity after gel …
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Study of porous magnetic nanocomposites for bio-catalysis and drug delivery
… immobilised lipases were used in hydrolysis of pNPP and hydrolysis of cis-3,5-diacetoxy-1-cyclopentene to investigate the catalytic activity of the immobilized enzymes compared to free enzymes. The results indicated that free lipases provided slightly higher conversion than immobilised lipases …
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Development of microelectrode arrays for real-time monitoring of alkaline phosphatase release from cells in clinical analysis
… at 30 minutes incubation time and at 6 mM pNPP concentration. Electrochemical analysis allowed the highest activity after 10 minutes incubation with concentration of pAPP of 9.6 mM. Activity of ALP during the proposed differentiation strategy showed a significant difference in the values of …
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Mechanisms of polyphosphate degradation
The goal of my research has been to investigate the mechanisms by which inorganic polyphosphate (polyP) is degraded in vivo. PolyP is a linear chain of phosphate moieties linked through high-energy phosphoanhydride bonds and can range in size from three-phosphates-long to thousands. Naturally, …