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Showing 1 to 18 of 18 for “"PMCA"”.
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PMCA as a regulator of calcium/calmodulin-dependent signal transduction pathways
… calcium/calmodulin-dependent calcium ATPases (PMCAs) are high affinity calcium pumps regulating many calcium-dependent processes and advances in its characterisation have discovered that it may play a novel role in signal transduction pathways. It was the aim of this work to further …
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Investigating the expression of plasma membrane calcium ATPase (PMCA) in patient-derived glioblastoma cultures
… the role of Plasma Membrane Calcium ATPases (PMCAs) in the pathology of glioblastoma, with a specific focus on isoforms PMCA1, PMCA2, and PMCA4. Patient-derived glioblastoma cultures and biopsy samples were employed to investigate PMCA expression patterns at both the transcript and protein …
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Molecular and cellular mechanisms implicated in the regulation of cellular PMCA expression during pulmonary arterial hypertension
… regulators. The Plasma Membrane Calcium ATPase (PMCA) proteins are high affinity pumps which extrude calcium ions extracellularly. Four PMCA Genes have been characterized (PMCA 1, 2, 3 and 4). PMCA 1 and 4 are highly expressed in the vasculature particularly, in endothelial and smooth muscle …
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Development of A High-Throughput System For Screening of Anti-Prion Molecules
… Misfolding Cyclic Amplification technology (PMCA, an in vitro test that replicates minimum quantities of infectious prions) has been modified to screen for small molecules inhibiting prion protein misfolding in a strain-specific manner. In order to approach a high-throughput PMCA system, …
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Untersuchung der funktionellen Interaktion zwischen der sarkolemmalen Calcium-ATPase und der neuronalen NO-Synthase
Die Plasmamembrancalcium-ATPase (PMCA) konnte als Interaktionspartner und Inhibitor der neuronalen NO-Synthase (nNOS) in Kardiomyozyten identifiziert werden. PMCA und nNO-Synthase kommen in Caveolae von Kardiomyocyten vor. Humane PMCA4b überexprimierende Rattenherzen zeigen ex vivo eine signifikant …
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Die Plasmamembran-Kalzium-ATPase im Myokard
Die Plasmamembran Kalzium-ATPase (PMCA) ist ein in den meisten eukaryontischen Zellen exprimiertes Enzym. Sie katalysiert den Transport von Kalziumionen aus der Zelle und besitzt gegenüber Kalzium eine hohe Affinität jedoch geringe Transportkapazität. Trotz der guten biochemischen Charakterisierung …
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Use of the Protein Misfolding Cyclic Amplification for food safety and drug discovery
… of the Protein Misfolding Cyclic Amplification (PMCA) technique to address these challenges in two key areas: (1) detecting CWD prions in processed meats subjected to common cooking procedures, and (2) identifying potential anti-prion compounds through a high-throughput PMCA adaptation.</p> …
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Targeting the calcium ATPase to the endoplasmic reticulum
… counterpart; plasma membrane calcium ATPase (PMCA).<br/>Replacing the C-terminus of SERCA with corresponding PMCA sequence results in<br/>mistargeting of the protein to the plasma membrane. The opposite construct (consisting of<br/>PMCA with the C-terminus replaced by that of SERCA) is located …
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Integrating Pro-Environmental Behavior with Transportation Network Modeling: User and System Level Strategies, Implementation, and Evaluation
… we propose personal mobility carbon allowance (PMCA) scheme to reduce emissions from personal travel. PMCA is a market-based scheme that allocates carbon credits to users at no cost based on the emissions reduction goal of the system. Users can spend carbon credits for travel and a market place …
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Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements
… that activates over hundred of targets including PMCA. This dissertation mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions. First we evaluated the extent of interaction of fluorophores with CaM upon conjugation. In this …
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Beteiligung der Plasmamembran Ca2+-ATPase an Wachstumsregulation und Signaltransduktion im Überexpressionsmodell und im Myokard transgener Ratten
Einleitung: Die Plasmamembran Ca2+-ATPase (PMCA) ist ein ubiquitär in eukaryonten Zellen vorkommendes Enzym, das Kalziumionen aus der Zelle transportiert. In Myokardzellen ist ihre Funktion trotz eingehender biochemischer Charakterisierung unklar. Die frühere Hypothese einer Zuständigkeit für die …
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Regulation des Tonus glatter Gefäßmuskelzellen bei die hPMCA4b überexprimierenden Mäusen
… mit der Rolle der sarkolemmalen Kalzium-ATPase PMCA (plasma membrane calmodulin-dependent calcium ATPase), die Kalziumionen aus der Zelle pumpt. Insbesondere ihre physiologische Relevanz neben dem sehr effektiven Na+/Ca2+-Austauscher ist spekulativ. Transgene Mäuse, die die humane Isoform 4b der …
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TRANSCRIPTIONAL CONTROL OF Ca2+ SIGNALING IN T CELLS
… (STIM1) inhibits plasma membrane Ca2+/ATPase 4 (PMCA4) function during T cell activation resulting in sustained elevation of Ca2+ signals(Ritchie, Samakai, & Soboloff, 2012). This interaction requires upregulation of both STIM1 and PMCA4. In this thesis, I hypothesize that changes in Ca2+ signals …
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Prions Propagate as Dynamic Strain Mixtures
… called protein misfolding cyclic amplification (PMCA) allows for detection of minute quantities of PrPSc. Combined with differences in conformational stability between strains which allow for the selective amplification and detection of biochemically distinct substrains. There are important …
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Evaluación de impacto en salud de políticas públicas: el plan de calidad del aire en la zona industrial bahía de Algeciras
… ejecución y puesta en marcha de las medidas del PMCA supondría salvar unas 23 vidas al año (IC 95%: 15; 30), debido a la reducción de los niveles de partículas en el aire ambiente. Si esta reducción fuese mayor y los niveles de partículas alcanzasen los niveles guía de la OMS, se salvarían unas …
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Age-associated changes to calcium handling proteins across the whole heart
… (NCX) and plasma membrane Ca²⁺ ATPase pumps (PMCA) were analysed in the SA node, right atrium, left atrium, left ventricle and right ventricle.The effect of ageing of the SA node was assessed via recordings of intrinsic heart rate in response to isoprenaline, a β-adrenergic stimulator, and …
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Metabolic Profiling of Prions In The Gastro-Intestinal Tract
<p><strong>Metabolic profiling of prions in the gastro-intestinal tract</strong><strong></strong></p> <p><strong>Uffaf Khan, B.S.</strong></p> <p><strong>Thesis Advisor: Claudio Soto, Ph.D.</strong></p> <p><strong>Abstract</strong></p> <p>Prion diseases, also known as transmissible spongiform …
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Role of the Plasma Membrane Calcium ATPase as a Negative Regulator of Angiogenesis
… is the plasma membrane calcium ATPases (PMCAs). These high affinity calcium extrusion pumps have been shown to interact with calcineurin in mammalian cells and cardiomyocytes and down-regulate the calcineurin/NFAT pathway. This is hypothesised to be due to the interaction between the two …